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Human TFF3 Antibody

Catalog #: MAB4408 4 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4408
MAB4408-SP
Product Details
FAQs
Supplemental Products
Reviews (4)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TFF3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TFF3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human TFF2.
Source
Monoclonal Mouse IgG2B Clone # 415921
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TFF3
Ala21-Phe80
Accession # Q07654
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human TFF3

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TFF3

Trefoil Factor 3 (TFF3) is one of three trefoil peptides secreted by epithelial cells that line mucus membranes. Trefoils contribute to protection and repair of the gastrointestinal tract. TFF3 is secreted by mucus-producing goblet cells in the large and small intestines and lower respiratory tract. It is mainly active as a disulfide-linked homodimer. The mature 59 amino acid (aa), 6.5 kDa human TFF3 contains one trefoil structure formed by intramolecular disulfides and shows 76% aa identity with mouse TFF3.

Long Name
Trefoil Factor 3
Entrez Gene IDs
7033 (Human); 21786 (Mouse); 25563 (Rat)
Alternate Names
HITF, human intestinal trefoil factor10Intestinal trefoil factor; ITF; TFF3; TFI; trefoil factor 3 (intestinal)

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Reviews for Human TFF3 Antibody

Average Rating: 3.8 (Based on 4 Reviews)

5 Star
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4 Star
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3 Star
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Human TFF3 Antibody
By Anonymous on 01/14/2021
Application: Microarrays Sample Tested: EDTA Plasma Species: Human
Other Human TFF3 Antibody MAB4408
Human TFF3 Antibody
By Anonymous on 02/07/2020
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma

Microarray Human TFF3 Antibody MAB4408
Human TFF3 Antibody
By Anonymous on 11/11/2018
Application: ELISA Sample Tested: Serum and Plasma Species: Human
Human TFF3 Antibody
By Anonymous on 11/07/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human