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Human TFPI-2 Antibody

Catalog #: MAB25451
1 Citation Datasheet / COA / SDS
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MAB25451
MAB25451-SP
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TFPI-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TFPI‑2 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 243227
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TFPI‑2
Asp23-Phe235
Accession # P48307
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human TFPI‑2 (Catalog # 2545‑PI), see our available Western blot detection antibodies
Neutralization
Measured by its ability to neutralize Recombinant Human TFPI-2 (0.017 µg/mL, Catalog # 2545-PI) inhibition of Trypsin (0.025 µg/mL) cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (10 µM, Catalog # ES002). The Neutralization Dose (ND50) is typically 0.45 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TFPI-2

Human Tissue Factor Pathway Inhibitor 2 (TFPI-2), also known as placental protein 5 (PP5) and retinal pigment epithelial cell factor 1 (REF-1), is a secreted protein with a N-terminal acidic region, three Kunitz (K) domains (residues 36 to 86, 96 to 149 and 158 to 208) separated with by two linker regions, and a C-terminal basic region (1‑3). Expression of TFPI-2 is down-regulated in several cancers, which may contribute to tumor progression in these cancers (4). The purified Recombinant Human TFPI-2 ends at residue 213 and does not contain the last 22 residues (residues 214 to 235) in the C-terminal region. It inhibits the activity of Recombinant Human Coagulation Factor VII (Catalog # 2338-SE) in the presence of Recombinant Human Tissue Factor (Catalog # 2339-PA).

References
  1. Miyagi, Y. et al. (1994) J. Biochem. 116:939.
  2. Sprecher, C.A. et al. (1994) Proc. Natl. Acad. Sci. USA 91:3353.
  3. Tanaka, Y. et al. (2004) Invest. Ophthalmol. Vis. Sci. 45:245.
  4. Rollin, J. et al. (2005) Br, J. Cancer 92:775.
Long Name
Tissue Factor Pathway Inhibitor 2
Entrez Gene IDs
7980 (Human)
Alternate Names
Placental protein 5; PP5; PP5FLJ21164; REF-1; TFPI2; TFPI-2; TFPI-2REF1; tissue factor pathway inhibitor 2

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Citation for Human TFPI-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Syncytiotrophoblast microvesicles released from pre-eclampsia placentae exhibit increased tissue factor activity.
    Authors: Gardiner C, Tannetta DS, Simms CA, Harrison P, Redman CW, Sargent IL
    PLoS ONE, 2011-10-14;6(10):e26313.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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