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Human TFPI Antibody

Catalog #: MAB29741 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB29741
MAB29741-SP
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TFPI Antibody Summary

Species Reactivity
Human
Specificity
Detects human TFPI in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) Coagulation factor II, VII, XI, XIV, rhTFPI-2, recombinant mouse (rm) TFPI, rmTFPI-2, or rhVitronectin is observed.
Source
Monoclonal Mouse IgG2B Clone # 374718
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TFPI
Asp29-Lys282
Accession # P10646
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample

Human TFPI Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human TFPI Biotinylated Antibody (Catalog # BAF2974)

Standard: Recombinant Human TFPI Protein, CF (Catalog # 2974-PI)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TFPI

Human TFPI, also known as lipoprotein-associated coagulation inhibitor (LACI) and extrinsic pathway inhibitor (EPI), is a physiological inhibitor of extrinsic pathway of coagulation and has biological functions of anticoagulation and anti-inflammation (1). It is a secreted protein with a N-terminal acidic region, three Kunitz (K) domains separated with by two linker regions, and a C-terminal basic region (2). The first K domain (residues 54 to 104) inhibits coagulation factor VIIa complexed to tissue factor (TF). The second K domain (residues 125 to 175) inhibits factor Xa. The third K domain (residues 217 to 267) binds to heparin (3). The C-terminal basic region may have several functions. For example, it plays an important role in binding of TFPI to cell surfaces (2). The purified rhTFPI ends at residue 282 and does not contain the last 20 residues (residues 283 to 302) in the C-terminal region. It inhibits the activity of rhCoagulation Factor VII (R&D Systems, Catalog # 2338-SE) in the presence of rhTissue Factor (R&D Systems, Catalog # 2339-PA). Human TFPI shares 60%, 59% and 58% amino acid sequence identity with rat, mouse and canine TFPI, respectively.

References
  1. Bai, H. et al. (2005) Thromb Haemost. 93:1055.
  2. Bajaj, M.S. et al. (2001) Thromb Haemost. 86:959.
  3. Mine, S. et al. (2002) Biochemistry 41:78.
Long Name
Tissue Factor Pathway Inhibitor
Entrez Gene IDs
7035 (Human); 21788 (Mouse)
Alternate Names
convertin; EPI; EPITFPI1LACITFI; Extrinsic pathway inhibitor; LACI; Lipoprotein-associated coagulation inhibitor; TFPI; tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor); tissue factor pathway inhibitor

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FAQs

  1. Do the TFPI antibodies (Catalog #s MAB2974, MAB29741, and BAF2974) detect TFPI-alpha or TFPI-beta?

    • These antibodies were raised against Asp29-Lys282 of isoform 1 of Accession # P10646. Isoform 1 is also known as TFPI-alpha. We do not epitope map our antibodies, so we do not know where within Asp29-Lys282 these antibodies bind. Since TFPI-alpha and TFPI-beta share 100% homology from aa1-209, it is possible that our antibodies recognize both the alpha and beta forms of TFPI.

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