Human TFPI Biotinylated Antibody Summary
Asp29-Lys282
Accession # P10646
Applications
Human TFPI Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TFPI
Human TFPI, also known as lipoprotein-associated coagulation inhibitor (LACI) and extrinsic pathway inhibitor (EPI), is a physiological inhibitor of extrinsic pathway of coagulation and has biological functions of anticoagulation and anti-inflammation (1). It is a secreted protein with a N-terminal acidic region, three Kunitz (K) domains separated with by two linker regions, and a C-terminal basic region (2). The first K domain (residues 54 to 104) inhibits coagulation factor VIIa complexed to tissue factor (TF). The second K domain (residues 125 to 175) inhibits factor Xa. The third K domain (residues 217 to 267) binds to heparin (3). The C-terminal basic region may have several functions. For example, it plays an important role in binding of TFPI to cell surfaces (2). The purified rhTFPI ends at residue 282 and does not contain the last 20 residues (residues 283 to 302) in the C-terminal region. It inhibits the activity of Recombinant Human Coagulation Factor VII (Catalog # 2338-SE) in the presence of Recombinant Human Coagulation Factor III/Tissue Factor (Catalog # 2339-PA).
- Bai, H. et al. (2005) Thromb Haemost. 93:1055.
- Bajaj, M.S. et al. (2001) Thromb Haemost. 86:959.
- Mine, S. et al. (2002) Biochemistry 41:78.
Product Datasheets
Citation for Human TFPI Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Mechanistic Modeling of the Pharmacodynamic and Pharmacokinetic Relationship of Tissue Factor Pathway Inhibitor-Neutralizing Antibody (BAY 1093884) in Cynomolgus Monkeys
Authors: JM Gu, XY Zhao, T Schwarz, J Schuhmache, A Baumann, E Ho, B Subramanya, K Tran, T Myles, C Patel, M Koellnberg
AAPS J, 2017-05-17;0(0):.
FAQs
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Do the TFPI antibodies (Catalog #s MAB2974, MAB29741, and BAF2974) detect TFPI-alpha or TFPI-beta?
These antibodies were raised against Asp29-Lys282 of isoform 1 of Accession # P10646. Isoform 1 is also known as TFPI-alpha. We do not epitope map our antibodies, so we do not know where within Asp29-Lys282 these antibodies bind. Since TFPI-alpha and TFPI-beta share 100% homology from aa1-209, it is possible that our antibodies recognize both the alpha and beta forms of TFPI.
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