Human TfR2 Antibody

Catalog #: AF3120 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3120-100
AF3120-SP

Detection of Human TfR2 by Western Blot.

2 Images

All TfR2 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TfR2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TfR2 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TfR2
Arg105-Phe801
Accession # Q9UP52

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Simple Western

50 µg/mL

Human liver tissue and K562 human chronic myelogenous leukemia cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human TfR2 by Western Blot. Western blot shows lysates of human liver tissue and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human TfR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3120) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for TfR2 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

View Larger

Detection of Human TfR2 by Simple Western^TM. Simple Western lane view shows lysates of human liver tissue and K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for TfR2 at approximately 50 kDa (as indicated) using 50 µg/mL of Goat Anti-Human TfR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3120) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TfR2

Transferrin Receptor 2 (TfR2) is an iron-binding protein that is homologous to the transferrin receptor. Lack of active TfR2 has been associated with a rare form of hemochromatosis. The TfR2 alpha isoform is a type 2 transmembrane protein that is expressed in the liver and modulates hepcidin production in response to iron. It forms a 215 kDa disulfide-linked homodimer that is presumably glycated. The TfR2 beta isoform lacks the intracellular, transmembrane and part of the extracellular regions and is expressed ubiquitously. The extracellular portion of human TfR2 shares 86% aa identity with mouse.

Long Name

Transferrin Receptor 2

Entrez Gene IDs

7036 (Human); 50765 (Mouse)

Alternate Names

HFE3; HFE3transferrin receptor protein 2; MGC126368; TfR2; TFRC2; transferrin receptor 2