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Human TGF-beta 1 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
31.2 - 2,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and Recombinant TGF-ß1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 1 (5 plates): (Catalog # DY007) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 1.

The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4


Reagent Diluent: (Catalog # DY997), or 1.4% delipidized bovine serum, 0.05% Tween 20 in PBS, pH 7.2-7.4, 0.2 μm filtered.

Blocking Buffer: (Catalog # DY004), or 5% Tween 20 in PBS, pH 7.2-7.4, 0.2 μm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Activation Reagents Required:

Sample Activation Kit 1: 3 vials (10 mL/vial) of 1N HCL and 3 vials (10 mL/vial) of 1.2 N NaOH/1M HEPES (R&D Systems, Catalog # DY010).

Scientific Data

Human TGF-beta 1 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TGF-beta 1

Transforming Growth Factor Beta 1, 2, and 3 (TGF-beta 1, TGF-beta 2, and TGF-beta 3) are highly pleiotropic cytokines that virtually all cell types secrete. TGF-beta molecules are proposed to act as cellular switches that regulate processes such as immune function, proliferation, and epithelial-mesenchymal transition. Targeted deletions of these genes in mice show that each TGF-beta isoform has some non-redundant functions: TGF-beta 1 is involved in hematopoiesis and endothelial differentiation; TGF-beta 2 affects development of cardiac, lung, craniofacial, limb, eye, ear, and urogenital systems; and TGF-beta 3 influences palatogenesis and pulmonary development. The full range of in vitro biological activities of TGF-beta 5 has not yet been explored. However, TGF-beta 1, TGF-beta 2, TGF-beta 3, and TGF-beta 5 have been found to be largely interchangeable in an inhibitory bioassay, and it is anticipated that TGF-beta 5 will show a spectrum of activities similar to the other TGF-beta family members. To date, the production of TGF-beta 5 has only been demonstrated in Xenopus.

TGF-beta ligands are initially synthesized as precursor proteins that undergo proteolytic cleavage. The mature segments form active ligand dimers via a disulfide-rich core consisting of the characteristic 'cysteine knot'. TGF-beta signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta RIII) and a type II serine/threonine kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a type I serine/threonine kinase receptor, either ALK-1 or TGF-beta RI (also called ALK-5). The activated type I receptor phosphorylates and activates Smad proteins that regulate transcription. Use of other signaling pathways that are Smad-independent allows for distinct actions observed in response to TGF-beta in different contexts.

Long Name:
Transforming Growth Factor beta 1
Entrez Gene IDs:
7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine); 100033900 (Equine)
Alternate Names:
CEDLAP; DPD1; latency-associated peptide; TGF beta; TGF beta1; TGFB; TGFB1; TGF-beta 1 protein; TGFbeta 1; TGF-beta 1; TGFbeta; TGF-beta-1; transforming growth factor beta-1; transforming growth factor, beta 1

Assay Procedure

ACTIVATION REAGENT PREPARATION

To activate latent TGF-ß1 to the immunoreactive form, prepare the following solutions for acid activation and neutralization. The solutions may be stored in polypropylene bottles at room temperature for up to one month.

Caution: Wear protective clothing and safety glasses during preparation or use of these reagents. Refer to the appropriate MSDS before use.

1 N HCl (100 mL) - To 91.67 mL of deionized water, slowly add 8.33 mL of 12 N HCl. Mix well.

1.2 N NaOH/0.5 M HEPES (100 mL) - To 75 mL of deionized water, slowly add 12 mL of 10 N NaOH. Mix well. Add 11.9 g of HEPES. Mix well. Bring final volume to 100 mL with deionized water.

TGF-β1 SAMPLE ACTIVATION

To activate latent TGF-β1 to immunoreactive TGF-β1, follow the activation procedure outlined below. Assay samples after neutralization (pH 7.2-7.6). Use polypropylene test tubes.

Note: Do not activate the kit standards. The kit standards contain active recombinant TGF-β1.

Cell Culture Supernates Serum/Plasma
To 100 μL of cell culture supernate, add 20 μL of 1 N HCI. To 40 μL of serum/plasma, add 20 μL of 1 N HCI.
Mix well. Mix well.
Incubate 10 minutes at room temperature. Incubate 10 minutes at room temperature.
Neutralize the acidified sample by adding 20 μL of 1.2 N NaOH/0.5 M HEPES. Neutralize the acidified sample by adding 20 μL of 1.2 N NaOH/0.5 M HEPES.
Mix well. Mix well.
Assay immediately. Prior to the assay, dilute the activated sample 20-fold with Reagent Diluent.*
The concentration read off the standard curve must be multiplied by the dilution factor, 1.4. The concentration read off the standard curve must be multiplied by the appropriate dilution factor, 90.

 

*A suggested 20-fold dilution is 10 μL of activated sample + 190 μL of Reagent Diluent.

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody (to the working concentration stated in the product datasheet ) in PBS without carrier protein. Immediately coat a 96-well microplate with 100 µL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 µL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block each well of the microplate as recommended in the product datasheet. Incubate at room temperature for a minimum of 1 hour.

    Note: The recommended Reagent Diluent typically contains 1% BSA. Some DuoSet Development Kits require alternative blocking agents, or for plates to be blocked overnight with a higher percentage of BSA, please see the product datasheet for details.
     
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

 

PRECAUTION
The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.

Assay Procedure

  1. Add 100 µL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 µL of the Detection Antibody, diluted in Reagent Diluent (as recommended in the product datasheet), to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 µL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 µL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 µL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Human TGF-beta 1 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

192 Citations: Showing 1 - 10
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  1. Dynamic changes in immune cells in humanized liver metastasis and subcutaneous xenograft mouse models
    Authors: Bang, HJ;Lee, KH;Park, MS;Sun, EG;Cho, SH;Chung, IJ;Shim, HJ;Bae, WK;
    Scientific reports
    Species: Xenograft
    Sample Types: Serum
  2. The TGF? type I receptor kinase inhibitor vactosertib in combination with pomalidomide in relapsed/refractory multiple myeloma: a phase 1b trial
    Authors: Malek, E;Rana, PS;Swamydas, M;Daunov, M;Miyagi, M;Murphy, E;Ignatz-Hoover, JJ;Metheny, L;Kim, SJ;Driscoll, JJ;
    Nature communications
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Toxicity and efficacy of type I interferons on the ocular surface: in vitro, animal, and clinical studies
    Authors: Yun, YI;Ko, JH;Ryu, JS;Kim, S;Jeon, HS;Kim, N;Kim, MK;Oh, JY;
    The ocular surface
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Identification and characterization of plasma proteins associated with intra-amniotic inflammation and/or infection in women with preterm labor.
    Authors: Cho, HY;Lee, JE;Park, KH;Choi, BY;Lee, MJ;Jeong, DE;Shin, S;
    Scientific reports
    Species: Human
    Sample Types: Plasma
  5. Repeat controlled human Plasmodium falciparum infections delay bloodstream patency and reduce symptoms
    Authors: Ferrer, P;Berry, AA;Bucsan, AN;Prajapati, SK;Krishnan, K;Barbeau, MC;Rickert, DM;Guerrero, SM;Usui, M;Abebe, Y;Patil, A;Chakravarty, S;Billingsley, PF;Pa'ahana-Brown, F;Strauss, K;Shrestha, B;Nomicos, E;Deye, GA;Sim, BKL;Hoffman, SL;Williamson, KC;Lyke, KE;
    Nature communications
    Species: Human
    Sample Types: Plasma
  6. Novel paired CD13-negative (MT-50.1) and CD13-positive (MT-50.4) HTLV-1-infected T-cell lines with differential regulatory T cell-like activity
    Authors: Egawa, Y;Higuchi, T;Hashida, Y;Ueno, K;Kojima, K;Daibata, M;
    Scientific reports
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Macrophage MCT4 inhibition activates reparative genes and protects from atherosclerosis by histone H3 lysine 18 lactylation
    Authors: Zhang, Y;Jiang, H;Dong, M;Min, J;He, X;Tan, Y;Liu, F;Chen, M;Chen, X;Yin, Q;Zheng, L;Shao, Y;Li, X;Chen, H;
    Cell reports
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Nuclear factor-?B activation by transforming growth factor-?1 drives tumour microenvironment-mediated drug resistance in neuroblastoma
    Authors: Louault, K;Blavier, L;Lee, MH;Kennedy, RJ;Fernandez, GE;Pawel, BR;Asgharzadeh, S;DeClerck, YA;
    British journal of cancer
    Species: Human
    Sample Types: Cell Culture Supernates
  9. ADAMTSL2 mutations determine the phenotypic severity in Geleophysic Dysplasia
    Authors: Camarena, V;Williams, MM;Morales, AA;Zafeer, MF;Kilic, OV;Kamiar, A;Abad, C;Rasmussen, MA;Briski, LM;Peart, L;Bademci, G;Barbouth, DS;Smithson, S;Wang, G;Shehadeh, LA;Walz, K;Tekin, M;
    JCI insight
    Species: Mouse
    Sample Types: Cell Culture Supernates, Cell Lysates
  10. Characterization of platelet rich plasma in feline immunodeficiency virus-infected cats: Cell, and PDGF-BB and TGF-beta 1 growth factor analysis
    Authors: Miguel-Pastor, L;Satué, K;Chicharro, D;Damiá, E;Cuervo, B;Torres-Torrillas, M;Martins, E;Velasco-Martínez, MG;Carrillo, JM;Sopena, JJ;Cerón, JJ;Rubio, M;
    Research in veterinary science
    Species: Feline
    Sample Types: Platelet-Poor Plasma, Platelet-Rich Plasma
  11. Effects of calcitriol upon TGF-?s and their receptors in trophoblast cells
    Authors: Noyola-Martínez, N;Chirinos, M;Ramírez-Camacho, I;Escamilla-Bucio, JE;García-Olivares, M;Aragón-Hernández, JP;Segovia-Mendoza, M;Halhali, A;Barrera, D;
    Journal of reproductive immunology
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Neutralizing tumor-related inflammation and reprogramming of cancer-associated fibroblasts by Curcumin in breast cancer therapy
    Authors: Jalilian, E;Abolhasani-Zadeh, F;Afgar, A;Samoudi, A;Zeinalynezhad, H;Langroudi, L;
    Scientific reports
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Enhanced Intervertebral Disc Repair via Genetically Engineered Mesenchymal Stem Cells with Tetracycline Regulatory System
    Authors: Kim, Y;An, SB;Lee, SH;Lee, JJ;Kim, SB;Ahn, JC;Hwang, DY;Han, I;
    International journal of molecular sciences
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Unrevealed roles of extracellular enolase?1 (ENO1) in promoting glycolysis and pro?cancer activities in multiple myeloma via hypoxia?inducible factor 1?
    Authors: Chung, IC;Huang, WC;Huang, YT;Chen, ML;Tsai, AW;Wu, PY;Yuan, TT;
    Oncology reports
    Species: Human, Mouse
    Sample Types: Cell Culture Supernates
  15. Serum interleukin-6, procalcitonin, and C-reactive protein at hospital admission can identify patients at low risk for severe COVID-19 progression
    Authors: Zobel, CM;Wenzel, W;Krüger, JP;Baumgarten, U;Wagelöhner, T;Neumann, N;Foroutan, B;Müller, R;Müller, A;Rauschning, D;Schü beta ler, M;Scheit, L;Weinreich, F;Oltmanns, K;Keidel, F;Koch, M;Spethmann, S;Schreiner, M;
    Frontiers in microbiology
    Species: Human
    Sample Types: Serum
  16. Cullin 4B Ubiquitin Ligase Is Important for Cell Survival and Regulates TGF-?1 Expression in Pleural Mesothelioma
    Authors: Kreienbühl, J;Changkhong, S;Orlowski, V;Kirschner, MB;Opitz, I;Meerang, M;
    International journal of molecular sciences
    Species: Human
    Sample Types: Cell Culture Supernates
  17. The influence of calcitriol and methylprednisolone on podocytes function in minimal change disease in vitro model
    Authors: Grubczak, K;Starosz, A;Makowska, B;Parfienowicz, Z;Kr?towska, M;Naumnik, B;Moniuszko, M;
    Scientific reports
    Species: Human
    Sample Types: Cell Culture Supernatants
  18. Hypoxia Preconditioned Serum (HPS) Promotes Proliferation and Chondrogenic Phenotype of Chondrocytes In Vitro
    Authors: Jiang, J;Altammar, J;Cong, X;Ramsauer, L;Steinbacher, V;Dornseifer, U;Schilling, AF;Machens, HG;Moog, P;
    International journal of molecular sciences
    Species: Human
    Sample Types: Serum
  19. Serum TGF-?1 and CD14 Predicts Response to Anti-TNF-? Therapy in IBD
    Authors: Coufal, S;Kverka, M;Kreisinger, J;Thon, T;Rob, F;Kolar, M;Reiss, Z;Schierova, D;Kostovcikova, K;Roubalova, R;Bajer, L;Jackova, Z;Mihula, M;Drastich, P;Tresnak Hercogova, J;Novakova, M;Vasatko, M;Lukas, M;Tlaskalova-Hogenova, H;Jiraskova Zakostelska, Z;
    Journal of immunology research
    Species: Human
    Sample Types: Serum
  20. Mild Cognitive Impairment Is Associated with Enhanced Activation of Th17 Lymphocytes in Non-Alcoholic Fatty Liver Disease
    Authors: Fiorillo, A;Gallego, JJ;Casanova-Ferrer, F;Giménez-Garzó, C;Urios, A;Ballester, MP;Durbán, L;Rios, MP;Megías, J;San Miguel, T;Kosenko, E;Escudero-García, D;Benlloch, S;Felipo, V;Montoliu, C;
    International journal of molecular sciences
    Species: Human
    Sample Types: Plasma
  21. A Reconstructed Human Melanoma-in-Skin Model to Study Immune Modulatory and Angiogenic Mechanisms Facilitating Initial Melanoma Growth and Invasion
    Authors: Michielon, E;López González, M;Stolk, DA;Stolwijk, JGC;Roffel, S;Waaijman, T;Lougheed, SM;de Gruijl, TD;Gibbs, S;
    Cancers
    Species: Human
    Sample Types: Cell Culture Supernates
  22. Targeting the PAI-1 Mechanism with a Small Peptide Increases the Efficacy of Alteplase in a Rabbit Model of Chronic Empyema
    Authors: Florova, G;De Vera, CJ;Emerine, RL;Girard, RA;Azghani, AO;Sarva, K;Jacob, J;Morris, DE;Chamiso, M;Idell, S;Komissarov, AA;
    Pharmaceutics
    Species: Rabbit
    Sample Types: Pleural Fluid
  23. Periodontal Disease in Young Adults as a Risk Factor for Subclinical Atherosclerosis: A Clinical, Biochemical and Immunological Study
    Authors: S Cicmil, A Cicmil, V Pavlic, J Kruni?, D Sladoje Pu, D Bokonji?, M ?oli?
    Journal of Clinical Medicine, 2023-03-12;12(6):.
    Species: Human
    Sample Types: Serum
  24. TGF-beta is elevated in hyperuricemic individuals and mediates urate-induced hyperinflammatory phenotype in human mononuclear cells
    Authors: V Klück, G Cab?u, L Mies, F Bukkems, L van Emst, R Bakker, A van Caam, HINT conso, TO Cri?an, LAB Joosten
    Arthritis Research & Therapy, 2023-02-27;25(1):30.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. In vitro and in vivo applications of a universal and synthetic thermo-responsive drug delivery hydrogel platform
    Authors: H Gholizadeh, E Landh, DM Silva, A Granata, D Traini, P Young, A Fathi, S Maleknia, T Abrams, F Dehghani, H Xin Ong
    International journal of pharmaceutics, 2023-02-25;635(0):122777.
    Species: N/A
    Sample Types: Hydrogel Supernates
  26. In vitro and in vivo applications of a universal and synthetic thermo-responsive drug delivery hydrogel platform
    Authors: H Gholizadeh, E Landh, DM Silva, A Granata, D Traini, P Young, A Fathi, S Maleknia, T Abrams, F Dehghani, H Xin Ong
    International journal of pharmaceutics, 2023;635(0):122777.
    Species: N/A
    Sample Types: Hydrogel Supernates
  27. Rhinovirus Suppresses TGF-beta-GARP Presentation by Peripheral NK Cells
    Authors: S Krammer, Z Yang, H Mitländer, JC Grund, S Trump, S Mittler, S Zirlik, S Finotto
    Cells, 2022-12-28;12(1):.
    Species: Human
    Sample Types: Cell Culture Supernates
  28. Identification of Potential microRNA Panels for Male Non-Small Cell Lung Cancer Identification Using Microarray Datasets and Bioinformatics Methods
    Authors: A Harangu?, R Lajos, L Budisan, O Zanoaga, C Ciocan, C Bica, R Pirlog, I Simon, M Simon, C Braicu, I Berindan-N
    Journal of personalized medicine, 2022-12-13;12(12):.
    Species: Human
    Sample Types: Serum
  29. SARS-CoV-2 Spike triggers barrier dysfunction and vascular leak via integrins and TGF-beta signaling
    Authors: SB Biering, FT Gomes de S, LV Tjang, F Pahmeier, C Zhu, R Ruan, SF Blanc, TS Patel, CM Worthingto, DR Glasner, B Castillo-R, V Servellita, NTN Lo, MP Wong, CM Warnes, DR Sandoval, TM Clausen, YA Santos, DM Fox, V Ortega, AM Näär, RS Baric, SA Stanley, HC Aguilar, JD Esko, CY Chiu, JE Pak, PR Beatty, E Harris
    Nature Communications, 2022-12-09;13(1):7630.
    Species: Human
    Sample Types: Cell Culture Supernates
  30. Eribulin normalizes pancreatic cancer-associated fibroblasts by simulating selected features of TGFbeta inhibition
    Authors: T Luong, E Cukierman
    BMC Cancer, 2022-12-02;22(1):1255.
    Species: Human
    Sample Types: Cell Culture Supernates
  31. Substrate stiffness engineered to replicate disease conditions influence senescence and fibrotic responses in primary lung fibroblasts
    Authors: KEC Blokland, M Nizamoglu, H Habibie, T Borghuis, M Schuliga, BN Melgert, DA Knight, CA Brandsma, SD Pouwels, JK Burgess
    Frontiers in Pharmacology, 2022-11-03;13(0):989169.
    Species: Human
    Sample Types: Cell Culture Supernates
  32. Utilization of ex vivo tissue model to study skin regeneration following microneedle stimuli
    Authors: X Liu, R Barresi, M Kaminer, K Qian, F Thillou, M Bataillon, IC Liao, Q Zheng, C Bouez
    Scientific Reports, 2022-10-27;12(1):18115.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. Hepatocyte phosphatase DUSP22 mitigates NASH-HCC progression by targeting FAK
    Authors: C Ge, J Tan, X Dai, Q Kuang, S Zhong, L Lai, C Yi, Y Sun, J Luo, C Zhang, L Zhu, B Wang, M Xu
    Oncogene, 2022-10-08;13(1):5945.
    Species: Human
    Sample Types: Serum
  34. SerpinB3 drives cancer stem cell survival in glioblastoma
    Authors: A Lauko, J Volovetz, SM Turaga, D Bayik, DJ Silver, K Mitchell, EE Mulkearns-, DC Watson, K Desai, M Midha, J Hao, K McCortney, A Steffens, U Naik, MS Ahluwalia, S Bao, C Horbinski, JS Yu, JD Lathia
    Cell Reports, 2022-09-13;40(11):111348.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. Immortalised canine buccal epithelial cells' CXCL8 secretion is affected by allergen extracts, Toll-like receptor ligands, IL-17A and calcitriol
    Authors: M Pelst, C Höbart, H de Rooster, B Devriendt, E Cox
    Veterinary research, 2022-09-13;53(1):72.
    Species: Canine
    Sample Types: Cell Culture Supernates
  36. Minimal Development of Liver Fibrosis in Adult Tolerant Liver Transplant Recipients Late After Immunosuppressive Drug Weaning and Transplantation
    Authors: AA Duizendstr, RJ De Knegt, NMA Nagtzaam, MGH Betjes, WA Dik, NHR Litjens, J Kwekkeboom
    Transplantation Proceedings, 2022-09-11;0(0):.
    Species: Human
    Sample Types: Serum
  37. A TGFbetaR inhibitor represses keratin-7 expression in 3D cultures of human salivary gland progenitor cells
    Authors: EW Fowler, EJ van Venroo, RL Witt, X Jia
    Scientific Reports, 2022-09-02;12(1):15008.
    Species: Human
    Sample Types: Cell Culture Supernates
  38. Growth Factor Release within Liquid and Solid PRF
    Authors: K Zwittnig, B Kirnbauer, N Jakse, P Schlenke, I Mischak, S Ghanaati, S Al-Maawi, D Végh, M Payer, TA Zrnc
    Journal of Clinical Medicine, 2022-08-29;11(17):.
    Species: Human
    Sample Types: Serum
  39. IL-17A in Human Liver: Significant Source of Inflammation and Trigger of Liver Fibrosis Initiation.
    Authors: Kartasheva-Ebertz D, Gaston J, Lair-Mehiri L, Mottez E, Buivan T, Massault P, Scatton O, Gaujoux S, Vaillant J, Pol S, Lagaye S
    Int J Mol Sci, 2022-08-29;23(17):.
    Species: Human
    Sample Types: Tissue Culture Supernates
  40. Extracellular matrix of early pulmonary fibrosis modifies the polarization of alveolar macrophage
    Authors: Y Zhang, L Zhu, J Hong, C Chen
    International immunopharmacology, 2022-08-24;111(0):109179.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  41. Effects of estradiol on the virulence traits of Porphyromonas gingivalis
    Authors: KJ Demirel, AN Guimaraes, I Demirel
    Scientific Reports, 2022-08-16;12(1):13881.
    Species: Human
    Sample Types: Cell Culture Supernates
  42. Development of an Electrochemical CCL5 Chemokine Immunoplatform for Rapid Diagnosis of Multiple Sclerosis
    Authors: S Guerrero, E Sánchez-Ti, L Agüí, A González-C, P Yáñez-Sede, JM Pingarrón
    Biosensors, 2022-08-07;12(8):.
    Species: Human
    Sample Types: Serum
  43. Pro-cancerogenic effects of spontaneous and drug-induced senescence of ovarian cancer cells in vitro and in vivo: a comparative analysis
    Authors: S Rutecki, P Szulc, M Paku?a, P Uruski, A Radziemski, E Naumowicz, R Moszy?ski, A Tykarski, J Miku?a-Pie, K Ksi??ek
    Journal of ovarian research, 2022-07-26;15(1):87.
    Species: Human
    Sample Types: Cell Culture Supernates
  44. Melanoma Stem Cells Educate Neutrophils to Support Cancer Progression
    Authors: M Anselmi, F Fontana, M Marzagalli, N Gagliano, M Sommariva, P Limonta
    Cancers, 2022-07-13;14(14):.
    Species: Human
    Sample Types: Cell Culture Supernates
  45. Plasma proteomic analysis to identify potential biomarkers of histologic chorioamnionitis in women with preterm premature rupture of membranes
    Authors: JE Lee, K Dan, HJ Kim, YM Kim, KH Park
    PLoS ONE, 2022-07-07;17(7):e0270884.
    Species: Human
    Sample Types: Plasma
  46. Prediction of emergency cerclage outcomes in women with cervical insufficiency: The role of inflammatory, angiogenic, and extracellular matrix-related proteins in amniotic fluid
    Authors: KN Lee, KH Park, YM Kim, I Cho, TE Kim
    PLoS ONE, 2022-05-10;17(5):e0268291.
    Species: Human
    Sample Types: Amniotic Fluid
  47. Cytokine Profile and Anti-Inflammatory Activity of a Standardized Conditioned Medium Obtained by Coculture of Monocytes and Mesenchymal Stromal Cells (PRS CK STORM)
    Authors: JP Lapuente, A Blázquez-M, J Marco-Brua, G Gómez, P Desportes, J Sanz, P Fernández, M García-Gil, F Bermejo, JV San Martín, A Algaba, JC De Gregori, D Lapuente, A De Gregori, B Lapuente, MV Andrés, A Anel
    Biomolecules, 2022-03-31;12(4):.
    Species: Human
    Sample Types: Cell Culture Supernates
  48. Parathyroid Hormone Induces Human Valvular Endothelial Cells Dysfunction That Impacts the Osteogenic Phenotype of Valvular Interstitial Cells
    Authors: M Vadana, S Cecoltan, L Ciortan, RD Macarie, AC Mihaila, MM Tucureanu, AM Gan, M Simionescu, I Manduteanu, I Droc, E Butoi
    International Journal of Molecular Sciences, 2022-03-29;23(7):.
    Species: Human
    Sample Types: Cell Culture Supernates
  49. Role of Betaglycan in TGF-beta Signaling and Wound Healing in Human Endometriotic Epithelial Cells and in Endometriosis
    Authors: AN Mwaura, MA Riaz, JB Maoga, E Mecha, COA Omwandho, G Scheiner-B, I Meinhold-H, L Konrad
    Biology, 2022-03-26;11(4):.
    Species: Human
    Sample Types: Cell Culture Supernates
  50. Optimization of loading protocols for tissue engineering experiments
    Authors: YD Ladner, AR Armiento, EJ Kubosch, JG Snedeker, MJ Stoddart
    Scientific Reports, 2022-03-24;12(1):5094.
    Species: Human
    Sample Types: Cell Culture Supernates
  51. Therapeutic Effects of Platelet-Derived Extracellular Vesicles in a Bioengineered Tendon Disease Model
    Authors: AL Graça, RMA Domingues, I Calejo, M Gómez-Flor, ME Gomes
    International Journal of Molecular Sciences, 2022-03-09;23(6):.
    Species: Human
    Sample Types: Extracellular Vesicles
  52. Increased serum LOXL2 concentration in pelvic inflammatory disease with pelvic adhesion
    Authors: C Xie, B Tang, K Wu, Q Meng, F Wang
    Oncogene, 2022-03-04;22(1):59.
    Species: Human
    Sample Types: Serum
  53. Anti-inflammatory effects of an autologous gold-based serum therapy in osteoarthritis patients
    Authors: J Feldt, AJ Donaubauer, J Welss, U Schneider, US Gaipl, F Paulsen
    Scientific Reports, 2022-03-03;12(1):3560.
    Species: Human
    Sample Types: Serum
  54. Nucleoporin-93 reveals a common feature of aggressive breast cancers: robust nucleocytoplasmic transport of transcription factors
    Authors: NB Nataraj, A Noronha, JS Lee, S Ghosh, HR Mohan Raju, A Sekar, B Zuckerman, M Lindzen, E Tarcitano, S Srivastava, M Selitrenni, I Livneh, D Drago-Garc, O Rueda, C Caldas, S Lev, T Geiger, A Ciechanove, I Ulitsky, R Seger, E Ruppin, Y Yarden
    Cell Reports, 2022-02-22;38(8):110418.
    Species: Human
    Sample Types: Cell Culture Supernates
  55. Tethered TGF-beta1 in a Hyaluronic Acid-Based Bioink for Bioprinting Cartilaginous Tissues
    Authors: J Hauptstein, L Forster, A Nadernezha, J Groll, J Te beta mar, T Blunk
    International Journal of Molecular Sciences, 2022-01-15;23(2):.
    Species: Human
    Sample Types: Recombinant Protein
  56. Impact and Possible Mechanism(s) of Adipose Tissue-Derived Mesenchymal Stem Cells on T-Cell Proliferation in Patients With Rheumatic Disease
    Authors: E Kuca-Warna, M Olesi?ska, P Szcz?sny, E Kontny
    Frontiers in Physiology, 2022-01-13;12(0):749481.
    Species: Human
    Sample Types: Cell Culture Supernates
  57. Skin Substitute Preparation Method Induces Immunomodulatory Changes in Co-Incubated Cells through Collagen Modification
    Authors: J Holl, C Pawlukiani, J Corton Rui, D Groth, K Grubczak, HR Hady, J Dadan, J Reszec, S Czaban, C Kowalewski, M Moniuszko, A Eljaszewic
    Pharmaceutics, 2021-12-15;13(12):.
    Species: Human
    Sample Types: Cell Culture Supernates
  58. SARS-CoV-2 Spike triggers barrier dysfunction and vascular leak via integrins and TGF-beta signaling
    Authors: SB Biering, FTG de Sousa, LV Tjang, F Pahmeier, R Ruan, SF Blanc, TS Patel, CM Worthingto, DR Glasner, B Castillo-R, V Servellita, NTN Lo, MP Wong, CM Warnes, DR Sandoval, TM Clausen, YA Santos, V Ortega, HC Aguilar, JD Esko, CY Chui, JE Pak, PR Beatty, E Harris
    bioRxiv : the preprint server for biology, 2021-12-13;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  59. Intramammary treatment using allogeneic pure platelet-rich plasma in cows with subclinical mastitis caused by Gram-positive bacteria
    Authors: PC Duque-Madr, J Velasco-Bo, A Ceballos-M, C López, JU Carmona
    Scientific Reports, 2021-12-09;11(1):23737.
    Species: Bovine
    Sample Types: Plasma
  60. Longitudinal relationships between polycyclic aromatic hydrocarbons exposure and heart rate variability: Exploring the role of transforming�growth factor-beta in a general Chinese population
    Authors: J Ma, Q Tan, X Nie, M Zhou, B Wang, X Wang, M Cheng, Z Ye, Y Xie, D Wang, W Chen
    Journal of Hazardous Materials, 2021-11-15;0(0):127770.
    Species: Human
    Sample Types: Plasma
  61. Inhibition of miR-155 Promotes TGF-beta Mediated Suppression of HIV Release in the Cervical Epithelial Cells
    Authors: J Gokavi, S Sadawarte, A Shelke, U Kulkarni-K, M Thakar, V Saxena
    Viruses, 2021-11-12;13(11):.
    Species: Human
    Sample Types: Cell Culture Supernates
  62. Preparation and growth factor characterization of cord blood-derived plasma, serum, growth factor-rich plasma and induced serum
    Authors: ME Rhéaume, J Perreault, D Fournier, P Trépanier
    Cytokine, 2021-11-08;149(0):155756.
    Species: Human
    Sample Types: Serum
  63. Proteomic identification of novel plasma biomarkers associated with spontaneous preterm birth in women with preterm labor without infection/inflammation
    Authors: JE Lee, KH Park, HJ Kim, YM Kim, JW Choi, S Shin, KN Lee
    PLoS ONE, 2021-10-28;16(10):e0259265.
    Species: Human
    Sample Types: Plasma
  64. Latent TGFbeta-binding proteins regulate UCP1 expression and function via TGFbeta2
    Authors: D Halbgebaue, J Roos, JB Funcke, H Neubauer, BS Hamilton, E Simon, EZ Amri, KM Debatin, M Wabitsch, P Fischer-Po, D Tews
    Molecular Metabolism, 2021-09-01;53(0):101336.
    Species: Human
    Sample Types: Cell Culture Supernates
  65. Neutrophils promote T-cell activation through the regulated release of CD44-bound Galectin-9 from the cell surface during HIV infection
    Authors: G Dunsmore, EP Rosero, S Shahbaz, DM Santer, J Jovel, P Lacy, S Houston, S Elahi
    PloS Biology, 2021-08-19;19(8):e3001387.
    Species: Human
    Sample Types: Plasma
  66. Severe COVID-19 Patients Show an Increase in Soluble TNFR1 and ADAM17, with a Relationship to Mortality
    Authors: Y Palacios, A Ruiz, LA Ramón-Luin, R Ocaña-Guzm, O Barreto-Ro, A Sánchez-Mo, B Tecuatzi-C, AG Regalado-G, RD Pineda-Gud, A García-Mar, F Juárez-Her, JP Farias-Con, I Fricke-Gal, G Pérez-Rubi, R Falfán-Val, I Buendia-Ro, K Medina-Que, L Chavez-Gal
    International Journal of Molecular Sciences, 2021-08-05;22(16):.
    Species: Human
    Sample Types: Serum
  67. An In Vitro System to Study the Effect of Subchondral Bone Health on Articular Cartilage Repair in Humans
    Authors: T Hopkins, KT Wright, NJ Kuiper, S Roberts, P Jermin, P Gallacher, JH Kuiper
    Cells, 2021-07-27;10(8):.
    Species: Human
    Sample Types: Cell Culture Supernates
  68. Regulation of Cellular Senescence Is Independent from Profibrotic Fibroblast-Deposited ECM
    Authors: KEC Blokland, H Habibie, T Borghuis, GJ Teitsma, M Schuliga, BN Melgert, DA Knight, CA Brandsma, SD Pouwels, JK Burgess
    Cells, 2021-06-29;10(7):.
    Species: Human
    Sample Types: Cell Culture Supernates
  69. Serum protein triplet TGF-&beta1, TIMP-1, and YKL-40 serve as diagnostic and prognostic profile for astrocytoma
    Authors: R Urbanavi?i, R Zabitait?, A Kriš?iukai, VP Deltuva, D Skiriut?
    Scientific Reports, 2021-06-23;11(1):13100.
    Species: Human
    Sample Types: Serum
  70. Th17/Treg-Related Intracellular Signaling in Patients with Chronic Obstructive Pulmonary Disease: Comparison between Local and Systemic Responses
    Authors: JD Lourenço, WR Teodoro, DF Barbeiro, APP Velosa, LEF Silva, JB Kohler, AR Moreira, MV Aun, IC da Silva, FLA Fernandes, EM Negri, JL Gross, IFLC Tibério, JT Ito, FDTQS Lopes
    Cells, 2021-06-22;10(7):.
    Species: Human
    Sample Types: Tissue Homogenates
  71. Changes in liver steatosis in HIV-positive women are associated with the BMI, but not with biomarkers
    Authors: R Fernandez-, MW Plankey, D Ware, J Bordon
    Cytokine, 2021-05-12;0(0):155573.
    Species: Human
    Sample Types: Plasma
  72. The noncoding MIR100HG RNA enhances the autocrine function of transforming growth factor &beta signaling
    Authors: P Papoutsogl, DM Rodrigues-, A Morén, A Bergman, F Pontén, C Coulouarn, L Caja, CH Heldin, A Moustakas
    Oncogene, 2021-05-04;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  73. Precision targeting of the plasminogen activator inhibitor-1 mechanism increases efficacy of fibrinolytic therapy in empyema
    Authors: G Florova, RA Girard, AO Azghani, K Sarva, A Buchanan, S Karandasho, CJ DeVera, D Morris, M Chamiso, K Koenig, DB Cines, S Idell, AA Komissarov
    Physiological Reports, 2021-05-01;9(9):e14861.
    Species: Rabbit
    Sample Types: Pleural Fluid
  74. TGF&beta1 induces in-vitro and ex-vivo angiogenesis through VEGF production in human ovarian follicular fluid-derived granulosa cells during in-vitro fertilization cycle
    Authors: TH Lai, HT Chen, WB Wu
    Journal of reproductive immunology, 2021-03-20;145(0):103311.
    Species: Human
    Sample Types: Cell Culture Supernates
  75. The effects of resveratrol on the expression of VEGF, TGF-&beta, and MMP-9 in endometrial stromal cells of women with endometriosis
    Authors: T Arablou, N Aryaeian, S Khodaverdi, R Kolahdouz-, Z Moradi, N Rashidi, AA Delbandi
    Scientific Reports, 2021-03-15;11(1):6054.
    Species: Human
    Sample Types: Cell Culture Supernates
  76. Palladin isoforms 3 and 4 regulate cancer-associated fibroblast pro-tumor functions in pancreatic ductal adenocarcinoma
    Authors: JI Alexander, DB Vendramini, R Francescon, T Luong, J Franco-Bar, N Shah, JC Gardiner, E Nicolas, KS Raghavan, E Cukierman
    Scientific Reports, 2021-02-15;11(1):3802.
    Species: Human
    Sample Types: Cell Culture Supernates
  77. Downregulation of epithelial DUOX1 in Chronic Obstructive Pulmonary Disease contributes to disease pathogenesis
    Authors: C Schiffers, C van de Wet, RA Bauer, A Habibovic, M Hristova, CM Dustin, S Lambrichts, PM Vacek, EF Wouters, NL Reynaert, A van der Vl
    JCI Insight, 2021-01-25;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  78. Regulation of Fibroblast Activation Protein by Transforming Growth Factor Beta-1 in Glioblastoma Microenvironment
    Authors: E Krepela, Z Vanickova, P Hrabal, M Zubal, B Chmielova, E Balaziova, P Vymola, I Matrasova, P Busek, A Sedo
    International Journal of Molecular Sciences, 2021-01-21;22(3):.
    Species: Human
    Sample Types: Tissue Homogenates
  79. Human Adipose Tissue-Derived Mesenchymal Stromal Cells Inhibit CD4+ T Cell Proliferation and Induce Regulatory T Cells as Well as CD127 Expression on CD4+CD25+ T Cells
    Authors: A Fiori, S Uhlig, H Klüter, K Bieback
    Cells, 2021-01-01;10(1):.
    Species: Human
    Sample Types: Cell Culture Supernates
  80. Exploring the Role of C-C Motif Chemokine Ligand-2 Single Nucleotide Polymorphism in Pulmonary Tuberculosis: A Genetic Association Study from North India
    Authors: SK Biswas, M Mittal, E Sinha, V Singh, N Arela, B Bajaj, PK Tiwari, VM Katoch, KK Mohanty
    Journal of Immunology Research, 2020-12-16;2020(0):1019639.
    Species: Human
    Sample Types: Serum
  81. Patient-derived and artificial ascites have minor effects on MeT-5A mesothelial cells and do not facilitate ovarian cancer cell adhesion
    Authors: M Estermann, YL Huang, D Septiadi, D Ritz, CY Liang, F Jacob, B Drasler, A Petri-Fink, V Heinzelman, B Rothen-Rut
    PLoS ONE, 2020-12-03;15(12):e0241500.
    Species: Human
    Sample Types: Abdominal Fluid
  82. TGF-&beta in the Secretome of Irradiated Peripheral Blood Mononuclear Cells Supports In Vitro Osteoclastogenesis
    Authors: L Panahipour, Z Kargarpour, M Laggner, M Mildner, HJ Ankersmit, R Gruber
    Int J Mol Sci, 2020-11-13;21(22):.
    Species: Human
    Sample Types: Cell Culture Supernates
  83. Circulating biomarkers and outcomes from a randomised phase 2 trial of gemcitabine versus capecitabine-based chemoradiotherapy for pancreatic cancer
    Authors: F Willenbroc, CM Cox, EE Parkes, CS Wilhelm-Be, AG Abraham, R Owens, A Sabbagh, CM Jones, DLI Hughes, T Maughan, CN Hurt, EE O'Neill, S Mukherjee
    Br J Cancer, 2020-10-26;0(0):.
    Species: Human
    Sample Types: Plasma
  84. GLP-1 receptor agonist ameliorates experimental lung fibrosis
    Authors: J Fandiño, L Toba, LC González-M, Y Diz-Chaves, F Mallo
    Sci Rep, 2020-10-22;10(1):18091.
    Species: Rat
    Sample Types: Plasma
  85. Regional hyperthermia enhances mesenchymal stem cell recruitment to tumor stroma: Implications for mesenchymal stem cell-based tumor therapy
    Authors: M Tutter, C Schug, KA Schmohl, S Urnauer, C Kitzberger, N Schwenk, M Petrini, C Zach, S Ziegler, P Bartenstei, WA Weber, G Multhoff, E Wagner, LH Lindner, PJ Nelson, C Spitzweg
    Mol Ther, 2020-10-15;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  86. The role of EMMPRIN/CD147 in regulating angiogenesis in patients with psoriatic arthritis
    Authors: MA Rahat, M Safieh, E Simanovich, E Pasand, T Gazitt, A Haddad, M Elias, D Zisman
    Arthritis Res Ther, 2020-10-14;22(1):240.
    Species: Human
    Sample Types: Serum
  87. Liposomes loaded with transforming growth factor &beta1 promote odontogenic differentiation of dental pulp stem cells
    Authors: L Jiang, WN Ayre, GE Melling, B Song, X Wei, AJ Sloan, X Chen
    J Dent, 2020-10-14;103(0):103501.
    Species: Human
    Sample Types: Cell Culture Supernates
  88. The NRF2 stimulating agent, tin protoporphyrin, activates protective cytokine pathways in healthy human subjects and in patients with chronic kidney disease
    Authors: RA Zager, ACM Johnson
    Physiol Rep, 2020-09-01;8(18):e14566.
    Species: Human
    Sample Types: Plasma
  89. Renal cell tumors convert natural killer cells to a proangiogenic phenotype
    Authors: Y Guan, CB Chambers, T Tabatabai, H Hatley, KR Delfino, K Robinson, SR Alanee, S Ran, DS Torry, A Wilber
    Oncotarget, 2020-06-30;11(26):2571-2585.
    Species: Human
    Sample Types: Serum
  90. Human interleukin-4-treated regulatory macrophages promote epithelial wound healing and reduce colitis in a mouse model
    Authors: TS Jayme, G Leung, A Wang, ML Workentine, S Rajeev, A Shute, BE Callejas, N Mancini, PL Beck, R Panaccione, DM McKay
    Sci Adv, 2020-06-05;6(23):eaba4376.
    Species: Human
    Sample Types: Cell Culture Supernates
  91. Immune modulation by complement receptor 3-dependent human monocyte TGF-beta1-transporting vesicles
    Authors: LD Halder, EAH Jo, MZ Hasan, M Ferreira-G, T Krüger, M Westermann, DI Palme, G Rambach, N Beyersdorf, C Speth, ID Jacobsen, O Kniemeyer, B Jungnickel, PF Zipfel, C Skerka
    Nat Commun, 2020-05-11;11(1):2331.
    Species: Human, Mouse
    Sample Types: Extracellular Vesicles
  92. Mesenchymal Stem and Stromal Cells Harness Macrophage-Derived Amphiregulin to Maintain Tissue Homeostasis
    Authors: JH Ko, HJ Kim, HJ Jeong, HJ Lee, JY Oh
    Cell Rep, 2020-03-17;30(11):3806-3820.e6.
    Species: Human
    Sample Types: Cell Culture Supernates
  93. Impaired Release of Neutrophil Extracellular Traps and Anemia-Associated T Cell Deficiency in Hereditary Hemorrhagic Telangiectasia
    Authors: F Droege, E Pylaeva, E Siakaeva, S Bordbari, I Spyra, K Thangavelu, C Lueb, M Domnich, S Lang, U Geisthoff, J Jablonska
    J Clin Med, 2020-03-12;9(3):.
    Species: Human
    Sample Types: Plasma
  94. Odontogenic infection by Porphyromonas gingivalis exacerbates fibrosis in NASH via hepatic stellate cell activation
    Authors: A Nagasaki, S Sakamoto, C Chea, E Ishida, H Furusho, M Fujii, T Takata, M Miyauchi
    Sci Rep, 2020-03-05;10(1):4134.
    Species: Human
    Sample Types: Cell Culture Supernates
  95. Anti-fibrotic effect of decorin in peritoneal dialysis and PD-associated peritonitis
    Authors: N Jiang, Q Zhang, MK Chau, MS Yip, SL Lui, S Liu, KM Chu, HY Ngan, TM Chan, S Yung
    EBioMedicine, 2020-02-12;52(0):102661.
    Species: Human
    Sample Types: Dialysate
  96. Are the Immune Properties of Mesenchymal Stem Cells from Wharton's Jelly Maintained during Chondrogenic Differentiation?
    Authors: C Voisin, G Cauchois, L Reppel, C Laroye, L Louarn, C Schenowitz, P Sonon, I Poras, V Wang, E D Carosell, N Benkirane-, P Moreau, N Rouas-Frei, D Bensoussan, C Huselstein
    J Clin Med, 2020-02-04;9(2):.
    Species: Human
    Sample Types: Cell Culture Supernates
  97. Growth Factors Delivery System for Skin Regeneration: An Advanced Wound Dressing
    Authors: M Nardini, S Perteghell, L Mastracci, F Grillo, G Marrubini, E Bari, M Formica, C Gentili, R Cancedda, ML Torre, M Mastrogiac
    Pharmaceutics, 2020-02-03;12(2):.
    Species: Human
    Sample Types: Cell Culture Supernates
  98. A-Kinase Anchoring Proteins Diminish TGF-&beta1/Cigarette Smoke-Induced Epithelial-To-Mesenchymal Transition
    Authors: H Zuo, M Trombetta-, IH Heijink, CHTJ van der Ve, L Hesse, KN Faber, WJ Poppinga, H Maarsingh, VO Nikolaev, AM Schmidt
    Cells, 2020-02-03;9(2):.
    Species: Human
    Sample Types: Cell Culture Supernates
  99. The Phenotype and Secretory Activity of Adipose-Derived Mesenchymal Stem Cells (ASCs) of Patients with Rheumatic Diseases
    Authors: E Kuca-Warna, U Skalska, I Janicka, U Musia?owic, K Bonek, P G?uszko, P Szcz?sny, M Olesi?ska, E Kontny
    Cells, 2019-12-17;8(12):.
    Species: Human
    Sample Types: Cell Culture Supernates
  100. Tensin1 expression and function in chronic obstructive pulmonary disease
    Authors: P Stylianou, K Clark, B Gooptu, D Smallwood, CE Brightling, Y Amrani, KM Roach, P Bradding
    Sci Rep, 2019-12-12;9(1):18942.
    Species: Human
    Sample Types: Cell Culture Supernates
  101. Blood cytokine, chemokine and growth factor profiling in a cohort of pregnant women from tropical countries
    Authors: C Dobaño, A Bardají, S Kochar, SK Kochar, N Padilla, M López, HW Unger, M Ome-Kaius, ME Castellano, M Arévalo-He, D Hans, FE Martínez-E, C Bôtto-Mene, A Malheiros, M Desai, A Casellas, CE Chitnis, S Rogerson, I Mueller, C Menéndez, P Requena
    Cytokine, 2019-09-09;125(0):154818.
    Species: Human
    Sample Types: Plasma
  102. Changes on the Structural Architecture and Growth Factor Release, and Degradation in Equine Platelet-Rich Fibrin Clots Cultured Over Time
    Authors: RF Jiménez-Ar, JU Carmona, M Prades
    J. Equine Vet. Sci., 2019-08-09;82(0):102789.
    Species: Equine
    Sample Types: Serum
  103. Inflammatory Bowel Disease Types Differ in Markers of Inflammation, Gut Barrier and in Specific Anti-Bacterial Response
    Authors: S Coufal, N Galanova, L Bajer, Z Gajdarova, D Schierova, Z Jiraskova, K Kostovciko, Z Jackova, Z Stehlikova, P Drastich, H Tlaskalova, M Kverka
    Cells, 2019-07-13;8(7):.
    Species: Human
    Sample Types: Serum
  104. Type 1 diabetic mellitus patients with increased atherosclerosis risk display decreased CDKN2A/2B/2BAS gene expression in leukocytes
    Authors: S Martínez-H, V Sánchez-Ga, A Herrero-Ce, Á Vinué, JT Real, JF Ascaso, DJ Burks, H González-N
    J Transl Med, 2019-07-12;17(1):222.
    Species: Human
    Sample Types: Plasma
  105. A Unique Pattern of Mesothelial-Mesenchymal Transition Induced in the Normal Peritoneal Mesothelium by High-Grade Serous Ovarian Cancer
    Authors: M Paku?a, P Uruski, A Niklas, A Wo?niak, D Szpurek, A Tykarski, J Miku?a-Pie, K Ksi??ek
    Cancers (Basel), 2019-05-13;11(5):.
    Species: Human
    Sample Types: Cell Culture Supernates
  106. Plasma Rich in Growth Factors (PRGF) Disrupt the Blood-Brain Barrier Integrity and Elevate Amyloid Pathology in the Brains of 5XFAD Mice
    Authors: QV Duong, ML Kintzing, WE Kintzing, IM Abdallah, AD Brannen, A Kaddoumi
    Int J Mol Sci, 2019-03-25;20(6):.
    Species: Human
    Sample Types: Plasma
  107. Effect of Propofol on the Production of Inflammatory Cytokines by Human Polarized Macrophages
    Authors: T Kochiyama, X Li, H Nakayama, M Kage, Y Yamane, K Takamori, K Iwabuchi, E Inada
    Mediators Inflamm., 2019-03-17;2019(0):1919538.
    Species: Human
    Sample Types: Cell Culture Supernates
  108. Large-scale secretome analyses unveil the superior immunosuppressive phenotype of umbilical cord stromal cells as compared to other adult mesenchymal stromal cells
    Authors: A Islam, I Urbarova, JA Bruun, I Martinez-Z
    Eur Cell Mater, 2019-02-20;37(0):153-174.
    Species: Human
    Sample Types: Cell Culture Supernates
  109. Inhalation of the prodrug PI3K inhibitor CL27c improves lung function in asthma and fibrosis
    Authors: CC Campa, RL Silva, JP Margaria, T Pirali, MS Mattos, LR Kraemer, DC Reis, G Grosa, F Copperi, EM Dalmarco, RCP Lima-Júnio, S Aprile, V Sala, F Dal Bello, DS Prado, JC Alves-Filh, C Medana, GD Cassali, GC Tron, MM Teixeira, E Ciraolo, RC Russo, E Hirsch
    Nat Commun, 2018-12-12;9(1):5232.
  110. PDGF enhances the protective effect of adipose stem cell-derived extracellular vesicles in a model of acute hindlimb ischemia
    Authors: T Lopatina, E Favaro, C Grange, M Cedrino, A Ranghino, S Occhipinti, S Fallo, F Buffolo, DA Gaykalova, MM Zanone, R Romagnoli, G Camussi
    Sci Rep, 2018-12-04;8(1):17458.
    Species: Human
    Sample Types: Cell Culture Supernates
  111. Four types of human platelet lysate, including one virally inactivated by solvent-detergent, can be used to propagate Wharton Jelly mesenchymal stromal cells
    Authors: MS Chen, TJ Wang, HC Lin, B Thierry
    New biotechnology, 2018-11-20;0(0):.
    Species: Human
    Sample Types: Platelet Lysates
  112. Combining Calcium Phosphates with Polysaccharides: A Bone-Inspired Material Modulating Monocyte/Macrophage Early Inflammatory Response
    Authors: H Rammal, C Bour, M Dubus, L Entz, L Aubert, SC Gangloff, S Audonnet, NB Bercu, F Boulmedais, C Mauprivez, H Kerdjoudj
    Int J Mol Sci, 2018-11-03;19(11):.
    Species: Human
    Sample Types: Cell Culture Supernates
  113. Inhibition of profibrotic microRNA-21 affects platelets and their releasate
    Authors: T Barwari, S Eminaga, U Mayr, R Lu, PC Armstrong, MV Chan, M Sahraei, M Fernández-, T Moreau, J Barallobre, M Lynch, X Yin, C Schulte, F Baig, R Pechlaner, SR Langley, A Zampetaki, P Santer, M Weger, R Plasenzott, M Schosserer, J Grillari, S Kiechl, J Willeit, AM Shah, C Ghevaert, TD Warner, C Fernández-, Y Suárez, M Mayr
    JCI Insight, 2018-11-02;3(21):.
    Species: Human
    Sample Types: Plasma
  114. LINK-A lncRNA promotes migration and invasion of ovarian carcinoma cells by activating TGF-? pathway
    Authors: J Ma, M Xue
    Biosci. Rep., 2018-09-13;0(0):.
    Species: Human
    Sample Types: Plasma
  115. Plasmodium falciparum Treated with Artemisinin-based Combined Therapy Exhibits Enhanced Mutation, Heightened Cortisol and TNF-? Induction
    Authors: AO Idowu, S Bhattachar, S Gradus, W Oyibo, Z George, C Black, J Igietseme, AA Azenabor
    Int J Med Sci, 2018-09-07;15(13):1449-1457.
    Species: Human
    Sample Types: Plasma
  116. Tumor associated macrophages induce epithelial to mesenchymal transition via the EGFR/ERK1/2 pathway in head and neck squamous cell carcinoma
    Authors: L Gao, W Zhang, WQ Zhong, ZJ Liu, HM Li, ZL Yu, YF Zhao
    Oncol. Rep., 2018-08-17;40(5):2558-2572.
  117. Butyrate produced by gut commensal bacteria activates TGF-beta1 expression through the transcription factor SP1 in human intestinal epithelial cells
    Authors: C Martin-Gal, F Béguet-Cre, L Marinelli, A Jamet, F Ledue, HM Blottière, N Lapaque
    Sci Rep, 2018-06-27;8(1):9742.
    Species: Human
    Sample Types: Cell Culture Supernates
  118. Crude leaf extracts of Piperaceae species downmodulate inflammatory responses by human monocytes
    Authors: AC Finato, TF Fraga-Silv, AUC Prati, AA de Souza J, BF Mazzeu, LG Felippe, RA Pinto, MA Golim, MSP Arruda, M Furlan, J Venturini
    PLoS ONE, 2018-06-20;13(6):e0198682.
    Species: Human
    Sample Types: Cell Culture Supernates
  119. Helicobacter pylori-derived heat shock protein 60 increases the induction of regulatory T-cells associated with persistent infection
    Authors: WT Hsu, SY Ho, TY Jian, HN Huang, YL Lin, CH Chen, TH Lin, MS Wu, CJ Wu, YL Chan, KW Liao
    Microb. Pathog., 2018-04-13;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  120. Exosomes Induce Fibroblast Differentiation into Cancer-associated Fibroblasts through TGF? Signaling
    Authors: C R Goulet, G Bernard, S Tremblay, S Chabaud, S Bolduc, F Pouliot
    Mol. Cancer Res., 2018-04-10;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  121. ??CD4effector/effector memory T cells differentiate into productively and latently infected central memory T cells by TGF?1 during HIV-1 infection
    Authors: KW Cheung, T Wu, SF Ho, YC Wong, L Liu, H Wang, Z Chen
    J. Virol., 2018-03-28;0(0):.
    Species: Bovine
    Sample Types: Media
  122. A model combining age, equivalent uniform dose and IL-8 may predict radiation esophagitis in patients with non-small cell lung cancer
    Authors: S Wang, J Campbell, MH Stenmark, P Stanton, J Zhao, MM Matuszak, RK Ten Haken, FM Kong
    Radiother Oncol, 2018-03-01;0(0):.
    Species: Human
    Sample Types: Plasma
  123. Comparative Analysis of Different Platelet Lysates and Platelet Rich Preparations to Stimulate Tendon Cell Biology: An In Vitro Study
    Authors: F Klatte-Sch, T Schmidt, M Uckert, S Scheffler, U Kalus, M Rojewski, H Schrezenme, A Pruss, B Wildemann
    Int J Mol Sci, 2018-01-10;19(1):.
    Species: Human
    Sample Types: Plasma
  124. Human M2 Macrophages Limit NK Cell Effector Functions through Secretion of TGF-? and Engagement of CD85j
    Authors: SY Nuñez, A Ziblat, F Secchiari, NI Torres, JM Sierra, XL Raffo Irao, RE Araya, CI Domaica, MB Fuertes, NW Zwirner
    J. Immunol., 2017-12-27;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  125. Rheumatoid arthritis bone marrow environment supports Th17 response.
    Authors: Ewa Kuca-Warna, Weronika Kurowska, Monika Prochorec, Anna Radzikows, Tomasz Burakowsk, Urszula Skalska, Magdalena Massalska, Magdalena Pleba?czy, Barbara Ma?dyk-No, Iwona S?owi?ska, Robert Gasik, W?odzimierz Ma?li?ski
    Arthritis Research & Therapy, 2017-12-08;0(0):1478-6362.
    Species: Human
    Sample Types: Plasma
  126. A Central Bioactive Region of LTBP-2 Stimulates the Expression of TGF-?1 in Fibroblasts via Akt and p38 Signalling Pathways
    Authors: MA Sideek, J Smith, C Menz, JRJ Adams, AJ Cowin, MA Gibson
    Int J Mol Sci, 2017-10-09;18(10):.
    Species: Human
    Sample Types: Cell Culture Supernates
  127. Activated Tissue-Resident Mesenchymal Stromal Cells Regulate Natural Killer Cell Immune and Tissue-Regenerative Function
    Authors: RM Petri, A Hackel, K Hahnel, CA Dumitru, K Bruderek, SB Flohe, A Paschen, S Lang, S Brandau
    Stem Cell Reports, 2017-08-03;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  128. Composition and functionality of the intrahepatic innate lymphoid cell-compartment in human non-fibrotic and fibrotic livers
    Authors: M Forkel, L Berglin, E Kekäläinen, A Carlsson, E Svedin, J Michaëlsso, M Nagasawa, JS Erjefält, M Mori, M Flodström-, A Bergquist, HG Ljunggren, M Westgren, U Lindforss, D Friberg, C Jorns, E Ellis, NK Björkström, J Mjösberg
    Eur. J. Immunol., 2017-07-11;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  129. Long-term cytokine and growth factor release from equine platelet-rich fibrin clots obtained with two different centrifugation protocols
    Authors: RF Jiménez-Ar, C López, ME Álvarez, C Giraldo, M Prades, JU Carmona
    Cytokine, 2017-06-23;97(0):149-155.
    Species: Equine
    Sample Types: Plasma
  130. Neutrophil Extracellular Traps Reprogram IL-4/GM-CSF-Induced Monocyte Differentiation to Anti-inflammatory Macrophages
    Authors: AB Guimarães-, NC Rochael, F Oliveira, J Echevarria, EM Saraiva
    Front Immunol, 2017-05-17;8(0):523.
    Species: Human
    Sample Types: Cell Culture Supernates
  131. In vitro and in vivo antagonistic activity of new probiotic culture against Clostridium difficile and Clostridium perfringens
    Authors: N Goli?, K Veljovi?, N Popovi?, J Djoki?, I Strahini?, I Mrvaljevi?, A Terzi?-Vid
    BMC Microbiol., 2017-05-06;17(1):108.
    Species: Human
    Sample Types: Cell Culture Supernates
  132. A nonsense mutation in TLR5 is associated with survival and reduced IL-10 and TNF-? levels in human Melioidosis
    Authors: P Chaichana, N Chantratit, F Brod, S Koosakulni, K Jenjaroen, S Chumseng, M Sumonwiriy, MN Burtnick, PJ Brett, P Teparrukku, D Limmathuro, NPJ Day, SJ Dunachie, TE West
    PLoS Negl Trop Dis, 2017-05-05;11(5):e0005587.
    Species: Human
    Sample Types: Plasma
  133. Reduction of relative centrifugal forces increases growth factor release within solid platelet-rich-fibrin (PRF)-based matrices: a proof of concept of LSCC (low speed centrifugation concept)
    Authors: K El Bagdadi, A Kubesch, X Yu, S Al-Maawi, A Orlowska, A Dias, P Booms, E Dohle, R Sader, CJ Kirkpatric, J Choukroun, S Ghanaati
    Eur J Trauma Emerg Surg, 2017-03-21;0(0):.
    Species: Human
    Sample Types: Complex Sample Type
  134. Extracellular Hsp90 and TGF? regulate adhesion, migration and anchorage independent growth in a paired colon cancer cell line model
    Authors: JA de la Mare, T Jurgens, AL Edkins
    BMC Cancer, 2017-03-16;17(1):202.
    Species: Human
    Sample Types: Cell Culture Supernates
  135. On the cytokine/chemokine network during Plasmodium vivax malaria: new insights to understand the disease
    Authors: NS Hojo-Souza, DB Pereira, FS de Souza, TA de Oliveir, MS Cardoso, MS Tada, GM Zanini, DC Bartholome, RT Fujiwara, LL Bueno
    Malar. J, 2017-01-24;16(1):42.
    Species: Human
    Sample Types: Plasma
  136. The differential expression of protease activated receptors contributes to functional differences between dark and fair keratinocytes
    Authors: Meilang Xue
    J. Dermatol. Sci, 2016-12-05;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  137. Tumor-Activated Mesenchymal Stromal Cells Promote Osteosarcoma Stemness and Migratory Potential via IL-6 Secretion
    PLoS ONE, 2016-11-16;11(11):e0166500.
    Species: Human
    Sample Types: Cell Culture Supernates
  138. A novel immunomodulatory function of neutrophils on rhinovirus-activated monocytes in vitro
    Authors: Brian G Oliver
    Thorax, 2016-06-10;71(11):1039-1049.
    Species: Human
    Sample Types: Cell Culture Supernates
  139. Differences in human mesenchymal stem cell secretomes during chondrogenic induction
    Authors: OF Gardner, N Fahy, M Alini, MJ Stoddart
    Eur Cell Mater, 2016-04-10;31(0):221-35.
    Species: Human
    Sample Types: Cell Culture Supernates
  140. Regulating VEGF signaling in platelet concentrates via specific VEGF sequestering
    Authors: DG Belair, NN Le, WL Murphy
    Biomater Sci, 2016-03-24;4(5):819-25.
    Species: Human
    Sample Types: Plasma
  141. Human Articular Cartilage Progenitor Cells Are Responsive to Mechanical Stimulation and Adenoviral-Mediated Overexpression of Bone-Morphogenetic Protein 2.
    Authors: Neumann A, Gardner O, Williams R, Alini M, Archer C, Stoddart M
    PLoS ONE, 2015-08-20;10(8):e0136229.
    Species: Human
    Sample Types: Cell Culture Supernates
  142. The TNF Family Molecules LIGHT and Lymphotoxin alphabeta Induce a Distinct Steroid-Resistant Inflammatory Phenotype in Human Lung Epithelial Cells.
    Authors: da Silva Antunes R, Madge L, Soroosh P, Tocker J, Croft M
    J Immunol, 2015-07-24;195(5):2429-41.
    Species: Human
    Sample Types: Cell Culture Supernates
  143. Lysosomal-associated Transmembrane Protein 4B (LAPTM4B) Decreases Transforming Growth Factor beta1 (TGF-beta1) Production in Human Regulatory T Cells.
    Authors: Huygens C, Lienart S, Dedobbeleer O, Stockis J, Gauthy E, Coulie P, Lucas S
    J Biol Chem, 2015-06-30;290(33):20105-16.
    Species: Human
    Sample Types: Cell Culture Supernates
  144. Pluripotency gene expression and growth control in cultures of peripheral blood monocytes during their conversion into programmable cells of monocytic origin (PCMO): evidence for a regulatory role of autocrine activin and TGF-beta.
    Authors: Ungefroren H, Hyder A, Hinz H, Groth S, Lange H, El-Sayed K, Ehnert S, Nussler A, Fandrich F, Gieseler F
    PLoS ONE, 2015-02-23;10(2):e0118097.
    Species: Human
    Sample Types: Cell Culture Supernates
  145. Antimony resistant Leishmania donovani but not sensitive ones drives greater frequency of potent T-regulatory cells upon interaction with human PBMCs: role of IL-10 and TGF-beta in early immune response.
    Authors: Guha R, Das S, Ghosh J, Sundar S, Dujardin J, Roy S
    PLoS Negl Trop Dis, 2014-07-17;8(7):e2995.
    Species: Human
    Sample Types: Cell Culture Supernates
  146. Immunomodulatory effects of bone marrow-derived mesenchymal stem cells on pro-inflammatory cytokine-stimulated human corneal epithelial cells.
    Authors: Wen L, Zhu M, Madigan M, You J, King N, Billson F, McClellan K, Sutton G, Petsoglou C
    PLoS ONE, 2014-07-08;9(7):e101841.
    Species: Human
    Sample Types: Cell Culture Supernates
  147. Asthmatic airway epithelial cells differentially regulate fibroblast expression of extracellular matrix components.
    Authors: Reeves S, Kolstad T, Lien T, Elliott M, Ziegler S, Wight T, Debley J
    J Allergy Clin Immunol, 2014-05-27;134(3):663-670.e1.
    Species: Human
    Sample Types: Cell Culture Supernates
  148. Diet-induced increase in plasma oxidized LDL promotes early fibrosis in a renal porcine auto-transplantation model.
    Authors: Chatauret N, Favreau F, Giraud S, Thierry A, Rossard L, Le Pape S, Lerman L, Hauet T
    J Transl Med, 2014-03-22;12(0):76.
    Species: Human
    Sample Types: Cell Culture Supernates
  149. Loss of WISP2/CCN5 in estrogen-dependent MCF7 human breast cancer cells promotes a stem-like cell phenotype.
    Authors: Ferrand N, Gnanapragasam A, Dorothee G, Redeuilh G, Larsen A, Sabbah M
    PLoS ONE, 2014-02-03;9(2):e87878.
    Species: Human
    Sample Types: Cell Culture Supernates
  150. TGF-beta-induced (TGFBI) protein in melanoma: a signature of high metastatic potential.
    Authors: Lauden L, Siewiera J, Boukouaci W, Ramgolam K, Mourah S, Lebbe C, Charron D, Aoudjit F, Jabrane-Ferrat N, Al-Daccak R
    J Invest Dermatol, 2014-01-17;134(6):1675-85.
    Species: Human
    Sample Types: Cell Culture Supernates
  151. Activin A as a mediator of NK-dendritic cell functional interactions.
    Authors: Seeger P, Bosisio D, Parolini S, Badolato R, Gismondi A, Santoni A, Sozzani S
    J Immunol, 2014-01-06;192(3):1241-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  152. TLR ligands stimulation protects MSC from NK killing.
    Authors: Giuliani M, Bennaceur-Griscelli A, Nanbakhsh A, Oudrhiri N, Chouaib S, Azzarone B, Durrbach A, Lataillade J
    Stem Cells, 2014-01-01;32(1):290-300.
    Species: Human
    Sample Types: Cell Culture Supernates
  153. Human neutrophil peptide-1 (HNP-1): a new anti-leishmanial drug candidate.
    Authors: Dabirian S, Taslimi Y, Zahedifard F, Gholami E, Doustdari F, Motamedirad M, Khatami S, Azadmanesh K, Nylen S, Rafati S
    PLoS Negl Trop Dis, 2013-10-17;7(10):e2491.
    Species: Human
    Sample Types: Cell Culture Supernates
  154. Differential chemokine and cytokine production by neonatal bovine gammadelta T-cell subsets in response to viral toll-like receptor agonists and in vivo respiratory syncytial virus infection.
    Authors: McGill J, Nonnecke B, Lippolis J, Reinhardt T, Sacco R
    Immunology, 2013-06-01;139(2):227-44.
    Species: Bovine
    Sample Types: Cell Culture Supernates
  155. Increase of O-glycosylated oncofetal fibronectin in high glucose-induced epithelial-mesenchymal transition of cultured human epithelial cells.
    Authors: Alisson-Silva F, Freire-de-Lima L, Donadio J, Lucena M, Penha L, Sa-Diniz J, Dias W, Todeschini A
    PLoS ONE, 2013-04-12;8(4):e60471.
    Species: Human
    Sample Types: Cell Culture Supernates
  156. Effects of the breed, sex and age on cellular content and growth factor release from equine pure-platelet rich plasma and pure-platelet rich gel.
    Authors: Giraldo C, Lopez C, Alvarez M, Samudio I, Prades M, Carmona J
    BMC Vet Res, 2013-02-12;9(0):29.
    Species: Equine
    Sample Types: Plasma
  157. Interaction with colon cancer cells hyperactivates TGF-beta signaling in cancer-associated fibroblasts.
    Authors: Hawinkels L, Paauwe M, Verspaget H, Wiercinska E, van der Zon J, van der Ploeg K, Koelink P, Lindeman J, Mesker W, ten Dijke P, Sier C
    Oncogene, 2012-12-03;33(1):97-107.
    Species: Human
    Sample Types: Cell Culture Supernates
  158. Pentraxin 3 (PTX3) expression in allergic asthmatic airways: role in airway smooth muscle migration and chemokine production.
    Authors: Zhang J, Shan L, Koussih L, Redhu NS, Halayko AJ, Chakir J, Gounni AS
    PLoS ONE, 2012-04-18;7(4):e34965.
    Species: Human
    Sample Types: Cell Culture Supernates
  159. Elevated adaptive immune responses are associated with latent infections of Wuchereria bancrofti.
    Authors: Arndts K, Deininger S, Specht S, Klarmann U, Mand S, Adjobimey T, Debrah AY, Batsa L, Kwarteng A, Epp C, Taylor M, Adjei O, Layland LE, Hoerauf A
    PLoS Negl Trop Dis, 2012-04-03;6(4):e1611.
    Species: Human
    Sample Types: Cell Culture Supernates
  160. Interleukin (IL)-4, IL-13, and IL-17A differentially affect the profibrotic and proinflammatory functions of fibrocytes from asthmatic patients.
    Authors: Bellini A, Marini MA, Bianchetti L, Barczyk M, Schmidt M, Mattoli S
    Mucosal Immunol, 2011-12-21;5(2):140-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  161. Increased secretion of Gas6 by smooth muscle cells in human atherosclerotic carotid plaques.
    Authors: Clauser S, Meilhac O, Bieche I, Raynal P, Bruneval P, Michel JB, Borgel D
    Thromb. Haemost., 2011-11-10;107(1):140-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  162. Human breast cancer cells enhance self tolerance by promoting evasion from NK cell antitumor immunity.
    Authors: Mamessier E, Sylvain A, Thibult ML, Houvenaeghel G, Jacquemier J, Castellano R, Goncalves A, Andre P, Romagne F, Thibault G, Viens P, Birnbaum D, Bertucci F, Moretta A, Olive D
    J. Clin. Invest., 2011-08-15;121(9):3609-22.
    Species: Human
    Sample Types: Cell Culture Supernates
  163. Enrichment of Foxp3+ CD4 regulatory T cells in migrated T cells to IL-6- and IL-8-expressing tumors through predominant induction of CXCR1 by IL-6.
    Authors: Eikawa S, Ohue Y, Kitaoka K
    J. Immunol., 2010-11-03;185(11):6734-40.
    Species: Human
    Sample Types: Pleural Effusion
  164. Soluble forms of VEGF receptor-1 and -2 promote vascular maturation via mural cell recruitment.
    Authors: Lorquet S, Berndt S, Blacher S, Gengoux E, Peulen O, Maquoi E, Noel A, Foidart JM, Munaut C, Pequeux C
    FASEB J., 2010-05-19;24(10):3782-95.
    Species: Human
    Sample Types: Cell Culture Supernates
  165. Secretion of angiogenic growth factors by villous cytotrophoblast and extravillous trophoblast in early human pregnancy.
    Authors: Lash GE, Naruse K, Innes BA, Robson SC, Searle RF, Bulmer JN
    Placenta, 2010-03-24;31(6):545-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  166. Anti-programmed cell death 1 antibody reduces CD4+PD-1+ T cells and relieves the lupus-like nephritis of NZB/W F1 mice.
    Authors: Kasagi S, Kawano S, Okazaki T, Honjo T, Morinobu A, Hatachi S, Shimatani K, Tanaka Y, Minato N, Kumagai S
    J. Immunol., 2010-02-05;184(5):2337-47.
    Species: Human
    Sample Types: Serum
  167. The roles of Wnt signaling modulators Dickkopf-1 (Dkk1) and Dickkopf-2 (Dkk2) and cell maturation state in osteogenesis on microstructured titanium surfaces.
    Authors: Olivares-Navarrete R, Hyzy S, Wieland M, Boyan BD, Schwartz Z
    Biomaterials, 2009-12-09;31(8):2015-24.
    Species: Human
    Sample Types: Cell Lysates
  168. Helminth antigens modulate immune responses in cells from multiple sclerosis patients through TLR2-dependent mechanisms.
    Authors: Correale J, Farez M
    J. Immunol., 2009-10-07;183(9):5999-6012.
    Species: Human
    Sample Types: Cell Culture Supernates
  169. TLR2-activated human langerhans cells promote Th17 polarization via IL-1beta, TGF-beta and IL-23.
    Authors: Aliahmadi E, Gramlich R, Grutzkau A, Hitzler M, Kruger M, Baumgrass R, Schreiner M, Wittig B, Wanner R, Peiser M
    Eur. J. Immunol., 2009-05-01;39(5):1221-30.
    Species: Human
    Sample Types: Cell Culture Supernates
  170. Normally occurring NKG2D+CD4+ T cells are immunosuppressive and inversely correlated with disease activity in juvenile-onset lupus.
    Authors: Dai Z, Turtle CJ, Booth GC, Riddell SR, Gooley TA, Stevens AM, Spies T, Groh V
    J. Exp. Med., 2009-03-16;206(4):793-805.
    Species: Human
    Sample Types: Cell Culture Supernates
  171. Growth factor regulation of growth factors in articular chondrocytes.
    Authors: Shi S, Mercer S, Eckert GJ, Trippel SB
    J. Biol. Chem., 2009-01-09;284(11):6697-704.
    Species: Human
    Sample Types: Whole Cells
  172. Detuning CD8+ T lymphocytes by down-regulation of the activating receptor NKG2D: role of NKG2D ligands released by activated T cells.
    Authors: Cerboni C, Ardolino M, Santoni A, Zingoni A
    Blood, 2009-01-05;113(13):2955-64.
    Species: Human
    Sample Types: Cell Culture Supernates
  173. Hyperexpression of NOD2 in intestinal mast cells of Crohn's disease patients: preferential expression of inflammatory cell-recruiting molecules via NOD2 in mast cells.
    Authors: Okumura S, Yuki K, Kobayashi R, Okamura S, Ohmori K, Saito H, Ra C, Okayama Y
    Clin. Immunol., 2008-10-19;130(2):175-85.
    Species: Human
    Sample Types: Cell Culture Supernates
  174. Glioblastoma-secreted factors induce IGFBP7 and angiogenesis by modulating Smad-2-dependent TGF-beta signaling.
    Authors: Pen A, Moreno MJ, Durocher Y, Deb-Rinker P, Stanimirovic DB
    Oncogene, 2008-08-18;27(54):6834-44.
    Species: Human
    Sample Types: Cell Culture Supernates
  175. Helminth infections associated with multiple sclerosis induce regulatory B cells.
    Authors: Correale J, Farez M, Razzitte G
    Ann. Neurol., 2008-08-01;64(2):187-99.
    Species: Human
    Sample Types: Cell Culture Supernates
  176. Human CD4(+) T cells recognize an epitope within alpha-fetoprotein sequence and develop into TGF-beta-producing CD4(+) T cells.
    Authors: Alisa A, Boswell S, Pathan AA, Ayaru L, Williams R, Behboudi S
    J. Immunol., 2008-04-01;180(7):5109-17.
    Species: Human
    Sample Types: Cell Culture Supernates
  177. Opposite regulation of transforming growth factors-beta2 and -beta3 expression in the human endometrium.
    Authors: Gaide Chevronnay HP, Cornet PB, Delvaux D, Lemoine P, Courtoy PJ, Henriet P, Marbaix E
    Endocrinology, 2007-11-26;149(3):1015-25.
    Species: Human
    Sample Types: Tissue Homogenates
  178. Tumor-associated leukemia inhibitory factor and IL-6 skew monocyte differentiation into tumor-associated macrophage-like cells.
    Authors: Duluc D, Delneste Y, Tan F, Moles MP, Grimaud L, Lenoir J, Preisser L, Anegon I, Catala L, Ifrah N, Descamps P, Gamelin E, Gascan H, Hebbar M, Jeannin P
    Blood, 2007-09-11;110(13):4319-30.
    Species: Human
    Sample Types: Cell Culture Supernates
  179. Transforming growth factor-beta1 suppresses airway hyperresponsiveness in allergic airway disease.
    Authors: Alcorn JF, Rinaldi LM, Jaffe EF, van Loon M, Bates JH, Janssen-Heininger YM, Irvin CG
    Am. J. Respir. Crit. Care Med., 2007-08-29;176(10):974-82.
    Species: Mouse
    Sample Types: BALF
  180. Human tumor-derived exosomes selectively impair lymphocyte responses to interleukin-2.
    Authors: Clayton A, Mitchell JP, Court J, Mason MD, Tabi Z
    Cancer Res., 2007-08-01;67(15):7458-66.
    Species: Human
    Sample Types: Cell Culture Supernates
  181. Airway remodelling in children with cystic fibrosis.
    Authors: Hilliard TN, Regamey N, Shute JK, Nicholson AG, Alton EW, Bush A, Davies JC
    Thorax, 2007-05-25;62(12):1074-80.
    Species: Human
    Sample Types: BALF
  182. Cancer immunoediting by GITR (glucocorticoid-induced TNF-related protein) ligand in humans: NK cell/tumor cell interactions.
    Authors: Baltz KM, Krusch M, Bringmann A, Brossart P, Mayer F, Kloss M, Baessler T, Kumbier I, Peterfi A, Kupka S, Kroeber S, Menzel D, Radsak MP, Rammensee HG, Salih HR
    FASEB J., 2007-03-14;21(10):2442-54.
    Species: Human
    Sample Types: Cell Culture Supernates
  183. Coordinated functions of E-cadherin and transforming growth factor beta receptor II in vitro and in vivo.
    Authors: Andl CD, Fargnoli BB, Okawa T, Bowser M, Takaoka M, Nakagawa H, Klein-Szanto A, Hua X, Herlyn M, Rustgi AK
    Cancer Res., 2006-10-15;66(20):9878-85.
    Species: Human
    Sample Types: Cell Culture Supernates
  184. Activation of transforming growth factor-beta by the integrin alphavbeta8 delays epithelial wound closure.
    Authors: Neurohr C, Nishimura SL, Sheppard D
    Am. J. Respir. Cell Mol. Biol., 2006-03-30;35(2):252-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  185. Effects of a short-course of amoxicillin/clavulanic acid on systemic and mucosal immunity in healthy adult humans.
    Authors: Dufour V, Millon L, Faucher JF, Bard E, Robinet E, Piarroux R, Vuitton DA, Meillet D
    Int. Immunopharmacol., 2005-05-01;5(5):917-28.
    Species: Human
    Sample Types: Feces
  186. Leukemia inhibitory factor is linked to regulatory transplantation tolerance.
    Authors: Metcalfe SM, Watson TJ, Shurey S, Adams E, Green CJ
    Transplantation, 2005-03-27;79(6):726-30.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  187. Association of type 2 cytokines with hepatic fibrosis in human Schistosoma mansoni infection.
    Authors: de Jesus AR, Magalhaes A, Miranda DG, Miranda RG, Araujo MI, de Jesus AA, Silva A, Santana LB, Pearce E, Carvalho EM
    Infect. Immun., 2004-06-01;72(6):3391-7.
    Species: Human
    Sample Types: Cell Culture Supernates
  188. The selective estrogen receptor modulator arzoxifene and the rexinoid LG100268 cooperate to promote transforming growth factor beta-dependent apoptosis in breast cancer.
    Authors: Rendi MH, Suh N, Lamph WW, Krajewski S, Reed JC, Heyman RA, Berchuck A, Liby K, Risingsong R, Royce DB, Williams CR, Sporn MB
    Cancer Res., 2004-05-15;64(10):3566-71.
    Species: Rat
    Sample Types: Cell Culture Supernates
  189. Effect of neutralizing transforming growth factor beta1 on the immune response against Mycobacterium tuberculosis in guinea pigs.
    Authors: Allen SS, Cassone L, Lasco TM
    Infect. Immun., 2004-03-01;72(3):1358-63.
    Species: Guinea Pig
    Sample Types: Pleural Effusion
  190. Presence of dendritic cells, MCP-1, and activated microglia/macrophages in amyotrophic lateral sclerosis spinal cord tissue.
    Authors: Henkel JS, Engelhardt JI, Siklos L, Simpson EP, Kim SH, Pan T, Goodman JC, Siddique T, Beers DR, Appel SH
    Ann. Neurol., 2004-02-01;55(2):221-35.
    Species: Human
    Sample Types: CSF
  191. Role of TGF-beta1 in relation to exercise-induced type I collagen synthesis in human tendinous tissue.
    Authors: Heinemeier K, Langberg H, Olesen JL, Kjaer M
    J. Appl. Physiol., 2003-08-15;95(6):2390-7.
    Species: Human
    Sample Types: Plasma
  192. Coordinate cytokine gene expression in vivo following induction of tuberculous pleurisy in guinea pigs.
    Authors: Allen SS, McMurray DN
    Infect. Immun., 2003-08-01;71(8):4271-7.
    Species: Guinea Pig
    Sample Types: Pleural Fluid

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Human TGF-beta 1 DuoSet ELISA
By Anonymous on 05/12/2021
Sample Tested: Cell culture supernatant

Human TGF-beta 1 DuoSet ELISA
By Anonymous on 03/29/2021
Sample Tested: Serum and Plasma

we used this kit to quantify human TGF beta 1 in human healthy controls sera, it produces a very good standard curve, levels of TGF beta in in healthy controls are very low.


Human TGF-beta 1 DuoSet ELISA
By Anonymous on 11/12/2020
Sample Tested: EDTA Plasma

Very good performance of the kit, we diluted our EDTA plasma samples 1:20 and applied the kit on a 384-well format. Very reliable and replicates are tight.


Human TGF-beta 1 DuoSet ELISA
By Anonymous on 10/05/2020
Sample Tested: Porcine Serum,Serum

Human TGF-beta 1 DuoSet ELISA
By Anonymous on 10/29/2019
Sample Tested: Serum-free Cell Culture Supernates

Should mention this kit detects active TGF beta1 only in the description.
Need buy activation kit for total TGF beta1 (including the latent form)


Human TGF-beta 1 DuoSet ELISA
By Tim Stachowski on 08/23/2019
Sample Tested: Purified Standard

Human TGF-beta 1 DuoSet ELISA
By Anonymous on 04/05/2019
Sample Tested: Cell Culture Media

Human TGF-beta 1 DuoSet ELISA
By Anonymous on 10/11/2018
Sample Tested: Serum and Plasma

Human TGF-beta 1 DuoSet ELISA
By Maria Yanez on 05/10/2018
Sample Tested: THP-1 human acute monocytic leukemia cell line

I differentiated my THP-1 cells to macrophages and polarized using IL-4 and IL-13. Then I checked the TGF-beta and i have in trouble to detect the concentration for some of my samples. Then I always verify my experiments, and for the other assays I detected tgf-ß


Human TGF-beta 1 DuoSet ELISA
By Maria Yanez on 07/17/2017
Sample Tested: THP-1 human acute monocytic leukemia cell line

Human TGF-beta 1 DuoSet ELISA
By Anonymous on 05/22/2017
Sample Tested: Adult lung

Human TGF-beta 1 DuoSet ELISA
By Anonymous on 04/03/2017
Sample Tested: Human EDTA plasma

Human TGF-beta 1 DuoSet ELISA
By Anonymous on 07/05/2016
Sample Tested: Human dental periodontal ligament fibroblasts