Human TGF-beta  RII Alexa Fluor® 700-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB241N-100
FAB241N-025
Detection of TGF‑ beta  RII in NS0 Mouse Cell Line Transfected with Human TGF‑ beta  RII by Flow Cytometry.
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Human TGF-beta  RII Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Human
Specificity
This antibody has been validated by flow cytometry on TGF-beta RII-transfected cells only. In contrast to Catalog # FAB2411A, minimal staining of PBMC lymphocytes was observed with this antibody.
Source
Monoclonal Mouse IgG1 Clone # 25508
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TGF-beta RII
Ile24-Asp159
Accession # P37173.2
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TGF-beta  RII antibody in NS0 Mouse Cell Line Transfected with Human TGF-beta  RII antibody by Flow Cytometry. View Larger

Detection of TGF‑ beta RII in NS0 Mouse Cell Line Transfected with Human TGF‑ beta RII by Flow Cytometry. NS0 mouse myeloma cell line transfected with human TGF-beta RII was stained with Mouse Anti-Human TGF-beta RII Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB241N, filled histogram) or isotype control antibody (Catalog # IC002N, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TGF-beta RII

TGF-beta RII is a membrane bound serine/threonine kinase. Upon ligand binding, TGF-beta RII interacts with TGF-beta RI to form the heteromeric signaling complex that transduces TGF-beta signals. A splice variant of the type II receptor, TGF-beta RIIb, containing a 25 amino acid residue insertion near the N-terminus of the mature protein has also been described.

Long Name
Transforming Growth Factor beta Receptor II
Entrez Gene IDs
7048 (Human); 21813 (Mouse)
Alternate Names
AAT3; EC 2.7.11; EC 2.7.11.30; FAA3; HNPCC6; LDS1B; LDS2B; MFS2; RIIC; TAAD2; tbetaR-II; TGF-beta receptor type II; TGF-beta receptor type IIB; TGF-beta receptor type-2; TGF-beta RII; TGF-beta type II receptor; TGFbetaRII; TGFbeta-RII; TGFBR2; TGF-bRII; TGFR-2; transforming growth factor beta receptor type IIC; transforming growth factor, beta receptor II (70/80kDa) isoform 1; transforming growth factor, beta receptor II (70/80kDa) isoform 2; transforming growth factor, beta receptor II (70/80kDa); transforming growth factor, beta receptor II (70-80kD); Transforming growth factor-beta receptor type II

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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