What epitope does this antibody detect?

Name: Human TGF-beta RIII Antibody
Brand: R&D Systems
SKU: MAB242
Price: 559 USD

We have not done epitope mapping, but the antibody was raised against the extracellular domain of recombinant human TGF -beta RIII, Gly 21-Asp781, accession # AAA67061. The antibody will react with the antigen in this region.

Human TGF-beta RIII Antibody

Catalog #: MAB242
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB242
MAB242-SP

All TGF-beta RIII Antibodies

Product Details

Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TGF-beta RIII Antibody Summary

Species Reactivity

Human

Specificity

Detects human TGF-beta RIII in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant rat MIS RII, recombinant human (rh) TGF-beta RI, rhTGF-beta RII, recombinant mouse TGF-beta RII, or rhTGF-beta RIIb.

Source

Monoclonal Mouse IgG₁ Clone # 31349

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TGF‑ beta RIII
Gly21-Asp781
Accession # AAA67061

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human TGF-beta RIII (Catalog # 242-R3)
under non-reducing conditions only

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta RIII

Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type I receptor is a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta. The Type II receptor is also a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I and Type II receptors together form a heterodimeric signaling complex that is essential for the transduction of the anti-proliferative signals of TGF-beta. The Type III receptor is a transmembrane proteoglycan with a large extracellular domain and a 43 amino acid residue cytoplasmic domain. The cytoplasmic domain of the Type III receptor lacks an obvious signaling motif and the receptor may not be involved directly in signal transduction. The Type III receptor binds TGF-beta 2 with the highest affinity. Other TGF-beta isoforms also bind the Type III receptor, but with lower affinities. Cellular responsiveness to TGF-beta 2 appears to be dependent on the presence of the Type III receptor which can interact with the signaling receptor complex. In addition to the transmembrane Type III receptor, a soluble form of the receptor is secreted by some cell types. The physiological role of this soluble receptor remains to be determined. The recombinant TGF-beta soluble receptor Type III binds the TGF-beta isoforms differentially in solution and exhibits TGF-beta antagonistic activities in vitro.

Long Name

Transforming Growth Factor beta Receptor III

Entrez Gene IDs

7049 (Human); 21814 (Mouse); 29610 (Rat)

Alternate Names

betaglycan proteoglycan; Betaglycan; BGCAN; TBRIII; TGF-beta receptor type 3; TGF-beta receptor type III; TGF-beta RIII; TGFbetaRIII; TGFBR3; TGF-bRIII; TGFR-3; Transforming growth factor beta receptor III; transforming growth factor beta receptor type 3; transforming growth factor, beta receptor III (betaglycan, 300kDa); transforming growth factor, beta receptor III

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Human TGF-beta RIII Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Attenuated TGF-beta1 responsiveness of dendritic cells and their precursors in atopic dermatitis.
Authors: Peng W, Maintz L, Allam J, Novak N
Eur J Immunol, 2013-04-09;43(5):1374-82.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

FAQs

What epitope does this antibody detect?
- We have not done epitope mapping, but the antibody was raised against the extracellular domain of recombinant human TGF -beta RIII, Gly 21-Asp781, accession # AAA67061. The antibody will react with the antigen in this region.

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