Home / TGF-beta RIII / Human TGF-beta  RIII Antibody

Human TGF-beta  RIII Antibody

Catalog #: AF-242-PB
18 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF-242-PB
AF-242-SP
TGF-beta RIII in Human Placenta Tissue.
3 Images
Product Details
Citations (18)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TGF-beta  RIII Antibody Summary

Species Reactivity
Human
Specificity
Detects human TGF-beta  RIII in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse (rm) TGF-beta  RIII is observed, and less than 1% cross-reactivity with recombinant human (rh) TGF-beta  RI, rhTGF-beta  RII, rhTGF-beta  RIIB, and rmTGF-beta  RI  is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TGF‑ beta  RIII
Gly21-Asp781
Accession # AAA67061
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human TGF-beta  RIII (Catalog # 242-R3)
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
1-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry TGF-beta RIII antibody in Human Placenta Tissue by Immunohistochemistry (IHC-P). View Larger

TGF-beta RIII in Human Placenta Tissue. TGF-beta RIII was detected in immersion fixed paraffin-embedded sections of human placenta tissue using Goat Anti-Human TGF-beta RIII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-242-PB) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface on syncytiotrophoblast cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Flow Cytometry Detection of TGF‑ beta RIII antibody in PC‑3 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of TGF‑ beta RIII in PC‑3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Goat Anti-Human TGF-beta RIII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-242-PB, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Fluorescein-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0109).

Flow Cytometry View Larger

Detection of TGF-beta RIII in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast adenocarcinoma cell line was stained with Goat Anti-Human TGF-beta RIII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-242-PB, filled histogram) or control antibody (AB-108-C, open histogram), followed by PE-conjugated Anti-Goat IgG Secondary Antibody (F0107).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta RIII

Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type I receptor is a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta. The Type II receptor is also a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I and Type II receptors together form a heterodimeric signaling complex that is essential for the transduction of the anti-proliferative signals of TGF-beta.

The Type III receptor is a transmembrane proteoglycan with a large extracellular domain and a 43 amino acid residue cytoplasmic domain. The cytoplasmic domain of the Type III receptor lacks an obvious signaling motif and the receptor may not be involved directly in signal transduction. The Type III receptor binds TGF-beta 2 with the highest affinity. Other TGF-beta isoforms also bind the Type III receptor, but with lower affinities. Cellular responsiveness to TGF-beta 2 appears to be dependent on the presence of the Type III receptor which can interact with the signaling receptor complex. In addition to the transmembrane Type III receptor, a soluble form of the receptor is secreted by some cell types. The physiological role of this soluble receptor remains to be determined. The recombinant TGF-beta soluble receptor Type III binds the TGF-beta isoforms differentially in solution and exhibits TGF-beta antagonistic activities in vitro.

Long Name
Transforming Growth Factor beta Receptor III
Entrez Gene IDs
7049 (Human); 21814 (Mouse); 29610 (Rat)
Alternate Names
betaglycan proteoglycan; Betaglycan; BGCAN; TBRIII; TGF-beta receptor type 3; TGF-beta receptor type III; TGF-beta RIII; TGFbetaRIII; TGFBR3; TGF-bRIII; TGFR-3; Transforming growth factor beta receptor III; transforming growth factor beta receptor type 3; transforming growth factor, beta receptor III (betaglycan, 300kDa); transforming growth factor, beta receptor III

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human TGF-beta  RIII Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Stromal heparan sulfate differentiates neuroblasts to suppress neuroblastoma growth.
    Authors: Knelson EH, Gaviglio AL, Nee JC et al.
    J Clin Invest
  2. ALK1 regulates the internalization of endoglin and the type III TGF-beta receptor
    Authors: Keren Tazat, Leslie Pomeraniec-Abudy, Melissa Hector-Greene, Szabina Szófia Szilágyi, Swati Sharma, Elise M. Cai et al.
    Molecular Biology of the Cell
  3. Evidence for a pathogenic role of extrafollicular, IL-10-producing CCR6+B helper T cells in systemic lupus erythematosus
    Authors: F Facciotti, P Larghi, R Bosotti, C Vasco, N Gagliani, C Cordiglier, S Mazzara, V Ranzani, E Rottoli, S Curti, A Penatti, B Karnani, Y Kobayashi, M Crosti, M Bombaci, JP van Hambur, G Rossetti, R Gualtierot, M Gerosa, S Gatti, S Torretta, L Pignataro, SW Tas, S Abrignani, M Pagani, F Grassi, PL Meroni, RA Flavell, J Geginat
    Proc. Natl. Acad. Sci. U.S.A., 2020-03-17;117(13):7305-7316.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Altering the Proteoglycan State of Transforming Growth Factor ? Type III Receptor (T?RIII)/Betaglycan Modulates Canonical Wnt/?-Catenin Signaling
    Authors: Karthikeyan Mythreye
    J. Biol. Chem., 2016-10-26;291(49):25716-25728.
    Applications: Immunoprecipitation, Western Blot
  5. Modulation of cerebral endothelial cell function by TGF-beta in glioblastoma: VEGF-dependent angiogenesis versus endothelial mesenchymal transition
    Authors: Shanmugarajan Krishnan, Emese Szabo, Isabel Burghardt, Karl Frei, Ghazaleh Tabatabai, Michael Weller
    Oncotarget
  6. TWIST1-Induced miR-424 Reversibly Drives Mesenchymal Programming while Inhibiting Tumor Initiation.
    Authors: Drasin D, Guarnieri A, Neelakantan D, Kim J, Cabrera J, Wang C, Zaberezhnyy V, Gasparini P, Cascione L, Huebner K, Tan A, Ford H
    Cancer Res, 2015-02-25;75(9):1908-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Role of TGF-beta receptor III localization in polarity and breast cancer progression
    Authors: Alison E. Meyer, Catherine E. Gatza, Tam How, Mark Starr, Andrew B. Nixon, Gerard C. Blobe
    Molecular Biology of the Cell
  8. The Balance of Cell Surface and Soluble Type III TGF-beta Receptor Regulates BMP Signaling in Normal and Cancerous Mammary Epithelial Cells1
    Authors: Catherine E. Gatza, Jennifer L. Elderbroom, Sun Young Oh, Mark D. Starr, Andrew B. Nixon, Gerard C. Blobe
    Neoplasia
  9. Molecular signatures and transcriptional regulatory networks of human immature decidual NK and mature peripheral NK cells.
    Authors: Wang F, Zhou Y, Fu B, Wu Y, Zhang R, Sun R, Tian Z, Wei H
    Eur J Immunol, 2014-06-11;44(9):2771-84.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Type III TGF-β receptor promotes FGF2-mediated neuronal differentiation in neuroblastoma
    Authors: Erik H. Knelson, Angela L. Gaviglio, Alok K. Tewari, Michael B. Armstrong, Karthikeyan Mythreye, Gerard C. Blobe
    Journal of Clinical Investigation
  11. Type III TGF-beta receptor downregulation generates an immunotolerant tumor microenvironment
    Authors: Brent A. Hanks, Alisha Holtzhausen, Katherine S. Evans, Rebekah Jamieson, Petra Gimpel, Olivia M. Campbell et al.
    Journal of Clinical Investigation
  12. Correlation of angiogenic biomarker signatures with clinical outcomes in metastatic colorectal cancer patients receiving capecitabine, oxaliplatin, and bevacizumab
    Authors: Yingmiao Liu, Mark D. Starr, Anuradha Bulusu, Herbert Pang, Nan Soon Wong, Wanda Honeycutt et al.
    Cancer Medicine
  13. Endocardial cell epithelial-mesenchymal transformation requires Type III TGF beta receptor interaction with GIPC
    Authors: Todd A. Townsend, Jamille Y. Robinson, Tam How, Daniel M. DeLaughter, Gerard C. Blobe, Joey V. Barnett
    Cellular Signalling
  14. Expression of growth factors and growth factor receptor in non-healing and healing ischaemic ulceration.
    Authors: Murphy MO, Ghosh J, Fulford P, Khwaja N, Halka AT, Carter A, Turner NJ, Walker MG
    Eur J Vasc Endovasc Surg, 2006-01-20;31(5):516-22.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  15. Inhibin alpha-subunit and the inhibin coreceptor betaglycan are downregulated in endometrial carcinoma.
    Authors: Florio P, Ciarmela P, Reis FM, Toti P, Galleri L, Santopietro R, Tiso E, Tosi P, Petraglia F
    Eur. J. Endocrinol., 2005-02-01;152(2):277-84.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  16. Transforming growth factor-beta1 regulation of growth zone chondrocytes is mediated by multiple interacting pathways.
    Authors: Rosado E, 2019, Schwartz Z, Sylvia VL, Dean DD, Boyan BD
    91, 2002-06-12;1590(1):1-15.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  17. TGF-beta1 regulates TGF-beta1 and FGF-2 mRNA expression during fibroblast wound healing.
    Authors: Song QH, Klepeis VE, Trinkaus-Randall V
    MP, Mol. Pathol., 2002-06-01;55(3):164-76.
    Species: Rabbit
    Sample Types: Whole Tissue
    Applications: IHC
  18. Extracellular domain of TGFbeta type III receptor inhibits angiogenesis and tumor growth in human cancer cells.
    Authors: Bandyopadhyay A, 2019, Zhu Y, Malik SN, Kreisberg J, Brattain MG, Sprague EA, Luo J, Lopez-Casillas F, Sun LZ
    101205, 2002-05-16;21(22):3541-51.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

FAQs

  1. What epitope does this antibody detect?

    • We have not done epitope mapping, but the antibody was raised against the extracellular domain of recombinant human TGF -beta RIII, Gly 21-Asp781, accession # AAA67061.  The antibody will react with the antigen in this region.

View all Antibody FAQs
Loading...

Reviews for Human TGF-beta  RIII Antibody

Average Rating: 3.5 (Based on 2 Reviews)

5 Star
0%
4 Star
50%
3 Star
50%
2 Star
0%
1 Star
0%

Have you used Human TGF-beta  RIII Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human TGF-beta  RIII Antibody
By Anonymous on 12/13/2019
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma

Microarray Human TGF-beta  RIII Antibody AF-242-PB
Human TGF-beta  RIII Antibody
By Anonymous on 11/07/2018
Application: ELISA Sample Tested: Plasma,Serum Species: Human and Mouse