Human Thimet Oligopeptidase/THOP1 Antibody

Catalog #: MAB3439 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3439
MAB3439-SP

All Thimet Oligopeptidase/THOP1 Antibodies

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Human Thimet Oligopeptidase/THOP1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Thimet Oligopeptidase/THOP1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross‑reactivity with recombinant human Neurolysin is observed.

Source

Monoclonal Mouse IgG₁ Clone # 455710

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Thimet Oligopeptidase/THOP1
Lys2-Cys689
Accession # P52888

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Thimet Oligopeptidase/THOP1 (Catalog # 3439-ZN)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Thimet Oligopeptidase/THOP1 (Catalog # 3439-ZN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thimet Oligopeptidase/THOP1

Thimet Oligopeptidase/THOP1, also known as endopeptidase EC 3.4.24.15 (EP24.15), is a zinc peptidase of the M3 family that also includes neurolysin/EC 3.4.24.16 and mitochondrial intermediate peptidase (1). Widely expressed by mammalian tissues and reported to present in different subcellular locations, THOP1 is primarily a cytoplasmic enzyme. It is capable of hydrolyzing a number of bioactive peptides and peptides released by the proteosome, limiting antigenic presentation by MHC class I molecules (1‑3). THOP1 also interacts with angiotensin II type I receptor and bradykinin B2 receptor (4). The amino acid sequence of human THOP1 is 90% and 86% identical to that of dog/mouse and rat, respectively.

References

Barrett, A.J. and J.-M. Chen (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. eds. p. 352, Elsevier Academic Press, San Diego.
Ray, K. et al. (2004) J. Biol. Chem. 279:20480.
Saric, T. et al. (2004) J. Biol. Chem. 279:46723.
Shivakumar, B.R. et al. (2005) Cell Biochem. Funct. 23:195.

Entrez Gene IDs

7064 (Human); 50492 (Mouse); 64517 (Rat)

Alternate Names

EC 3.4.24; EC 3.4.24.15; Endopeptidase 24.15; EP24.15; MEPD_HUMAN; MP78; thimet oligopeptidase 1; thimet oligopeptidase; THOP1; TOP

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