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Human Thimet Oligopeptidase/THOP1 Antibody

Catalog #: AF3439
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3439
AF3439-SP
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Thimet Oligopeptidase/THOP1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Oligopeptidase/THOP1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human Neurolysin is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Oligopeptidase/THOP1
Lys2-Cys689
Accession # P52888
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Thimet Oligopeptidase/THOP1 (Catalog # 3439-ZN)
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human kidney
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Thimet Oligopeptidase/THOP1 (Catalog # 3439-ZN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thimet Oligopeptidase/THOP1

Thimet Oligopeptidase/THOP1, also known as endopeptidase EC 3.4.24.15 (EP24.15), is a zinc peptidase of the M3 family that also includes neurolysin/EC 3.4.24.16 and mitochondrial intermediate peptidase (1). Widely expressed by mammalian tissues and reported to present in different subcellular locations, THOP1 is primarily a cytoplasmic enzyme. It is capable of hydrolyzing a number of bioactive peptides and peptides released by the proteosome, limiting antigenic presentation by MHC class I molecules (1‑3). THOP1 also interacts with angiotensin II type I receptor and bradykinin B2 receptor (4). The optimal activity of the purified THOP1 may or may not require the presence of a reducing agent, depending upon the source of the enzyme and the purification method used.

References
  1. Barrett, A.J. and J.-M. Chen (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. eds. p. 352, Elsevier Academic Press, San Diego.
  2. Ray, K. et al. (2004) J. Biol. Chem. 279:20480.
  3. Saric, T. et al. (2004) J. Biol. Chem. 279:46723.
  4. Shivakumar, B.R. et al. (2005) Cell Biochem. Funct. 23:195.
Entrez Gene IDs
7064 (Human); 50492 (Mouse); 64517 (Rat)
Alternate Names
EC 3.4.24; EC 3.4.24.15; Endopeptidase 24.15; EP24.15; MEPD_HUMAN; MP78; thimet oligopeptidase 1; thimet oligopeptidase; THOP1; TOP

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Citation for Human Thimet Oligopeptidase/THOP1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Thimet oligopeptidase as a potential CSF biomarker for Alzheimer's disease: A cross‐platform validation study
    Authors: Yanaika S. Hok‐A‐Hin, Katharina Bolsewig, Daimy N. Ruiters, Alberto Lleó, Daniel Alcolea, Afina W. Lemstra et al.
    Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring

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