Human Thrombomodulin/BDCA-3 ELISA Kit - Quantikine
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Summary
Recovery
Linearity
Data Examples
Product Datasheets
Preparation and Storage
Background
Related Research Areas
Human Thrombomodulin/BDCA-3 Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 uL), Cell Lysates (25 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL), Urine (10 uL)
Sensitivity
27 pg/mL
Assay Range
62.5 - 4,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
Specificity
Natural and recombinant human Thrombomodulin. This kit also recognizes Thrombomodulin complexed with Thrombin, Protein C, and Thrombin/Protein C.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human Thrombomodulin Immunoassay is a 4.5 hour solid phase ELISA designed to measure human Thrombomodulin in cell culture supernates, cell lysates, serum, plasma, and urine. It contains NS0-expressed recombinant human Thrombomodulin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Thrombomodulin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Thrombomodulin.
Recovery
The recovery of Thrombomodulin spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 96 | 88-106 |
| Citrate Plasma (n=4) | 94 | 85-102 |
| EDTA Plasma (n=4) | 95 | 86-105 |
| Heparin Plasma (n=4) | 91 | 85-96 |
| Serum (n=4) | 89 | 85-96 |
| Urine (n=4) | 99 | 85-115 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of Thrombomodulin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data
Product Datasheets
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Thrombomodulin/BDCA-3
Thrombomodulin (TM), also known as CD141 and BDCA3, is a transmembrane expressed on vascular endothelial cells (EC), arterial smooth muscle cells, monocytes, and macrophages. It binds Thrombin and enhances the Thrombin-mediated activation of the anti-coagulant Protein C and the anti-fibrinolytic TAFI/Carboxypeptidase B2. Thrombomodulin also inhibits the ability of Thrombin to activate several pro-coagulant proteins (e.g. Fibrinogen, Factor V, Factor XIII, PAR-1). Thrombomodulin exerts multiple anti-inflammatory effects and reduces the severity of disease involving chronic inflammation. In cancer, Thrombomodulin suppresses tumor angiogenesis, growth, and epithelial-mesenchymal transition. Soluble fragments of Thrombomodulin are elevated in the serum, urine, and synovial fluid during coagulation disorders, inflammation, and organ failure.
Entrez Gene IDs:
7056 (Human); 21824 (Mouse); 83580 (Rat)
Alternate Names:
BDCA3; BDCA-3; CD141 antigen; CD141; Fetomodulin; THBD; THRM; Thrombomodulin; TM
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
Assay Procedure
Refer to the product Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
100 µL Assay Diluent
50 µL Standard, Control, or Sample
200 µL Conjugate
200 µL Substrate Solution
50 µL Stop Solution
Citations for Human Thrombomodulin/BDCA-3 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
13
Citations: Showing 1 - 10
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Fluid bolus increases plasma hyaluronan concentration compared to follow-up strategy without a bolus in oliguric intensive care unit patients
Authors: Serlo, M;Inkinen, N;Lakkisto, P;Valkonen, M;Pulkkinen, A;Selander, T;Pettilä, V;Vaara, ST;
Scientific reports
Species: Human
Sample Types: Plasma, Urine
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Head-to-head comparison of four COVID-19 vaccines on platelet activation, coagulation and inflammation. The TREASURE study
Authors: M Brambilla, P Canzano, PD Valle, A Becchetti, M Conti, M Alberti, A Galotta, ML Biondi, PA Lonati, F Veglia, A Bonomi, N Cosentino, PL Meroni, GV Zuccotti, A D'Angelo, M Camera
Thrombosis Research, 2023-01-18;223(0):24-33.
Species: Human
Sample Types: Plasma
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Lung Ultrasound Findings and Endothelial Perturbation in a COVID-19 Low-Intensity Care Unit
Authors: R Gualtierot, F Tafuri, R Rossio, M Rota, P Bucciarell, B Ferrari, A Giachi, C Suffritti, M Cugno, F Peyvandi, On Behalf
Journal of Clinical Medicine, 2022-09-15;11(18):.
Species: Human
Sample Types: Plasma
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Different Profiles of Cytokines, Chemokines and Coagulation Mediators Associated with Severity in Brazilian Patients Infected with Dengue Virus
Authors: VE Fiestas So, NR da Costa F, CF Dos Santos, G Corrêa, MDC Cipitelli, M Dornelas R, LJ de Souza, PV Damasco, RV da Cunha, FB Dos Santos, LM de Oliveir, EL de Azeredo
Viruses, 2021-09-08;13(9):.
Species: Human
Sample Types: Plasma
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Study the relationship of endothelial damage / dysfunction due to occupational exposure to low dose ionizing radiation versus high dose exposure during radiotherapy
Authors: SA El-Benhawy, NA Sadek, MM Kamel, AM Sharaf, IG Abderhman, MI Morsi, A Abobakr
Cancer Treat Res Commun, 2020-10-08;25(0):100215.
Species: Human
Sample Types: Serum
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Permissive microbiome characterizes human subjects with a neurovascular disease cavernous angioma
Authors: SP Polster, A Sharma, C Tanes, AT Tang, P Mericko, Y Cao, J Carrión-Pe, R Girard, J Koskimäki, D Zhang, A Stadnik, SG Romanos, SB Lyne, R Shenkar, K Yan, C Lee, A Akers, L Morrison, M Robinson, A Zafar, K Bittinger, H Kim, JA Gilbert, ML Kahn, L Shen, IA Awad
Nat Commun, 2020-05-27;11(1):2659.
Species: Human
Sample Types: Plasma
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Enhanced effect of recombinant human soluble thrombomodulin by ultrasound irradiation in acute liver failure
Authors: K Hoshino, Y Nakamura, T Nakano, A Watanabe, H Sheng, K Tachibana, H Ishikura
Sci Rep, 2020-02-03;10(1):1742.
Species: Mouse
Sample Types: Tissue Homogenates
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Markers of endothelial cell activation and neutrophil extracellular traps are elevated in immune thrombocytopenia but are not enhanced by thrombopoietin receptor agonists
Authors: L Garabet, CE Henriksson, ML Lozano, W Ghanima, J Bussel, E Brodin, MP Fernández-, C Martínez, R González-C, MC Mowinckel, PM Sandset
Thromb. Res., 2019-11-30;185(0):119-124.
Species: Human
Sample Types: Plasma
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Relationship of mottling score, skin microcirculatory perfusion indices and biomarkers of endothelial dysfunction in patients with septic shock: an observational study
Authors: S Kazune, A Caica, K Volceka, O Suba, U Rubins, A Grabovskis
Crit Care, 2019-09-11;23(1):311.
Species: Human
Sample Types: Plasma
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Systemic Inflammation Precedes Microalbuminuria in Diabetes
Authors: FG Scurt, J Menne, S Brandt, A Bernhardt, PR Mertens, H Haller, C Chatzikyrk
Kidney Int Rep, 2019-06-21;4(10):1373-1386.
Species: Human
Sample Types: Serum
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Plasma irisin is elevated in type 2 diabetes and is associated with increased E-selectin levels
Authors: KS Rana, C Pararasa, I Afzal, DA Nagel, EJ Hill, CJ Bailey, HR Griffiths, I Kyrou, HS Randeva, S Bellary, JE Brown
Cardiovasc Diabetol, 2017-11-09;16(1):147.
Species: Human
Sample Types: Plasma
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Effects of Alloantibodies to Human Leukocyte Antigen on Endothelial Expression and Serum Levels of Thrombomodulin
Authors: S Béland, P Vallin, O Désy, E Lévesque, SA De Serres
J. Thromb. Haemost, 2017-03-21;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Common genetic risk factors for venous thrombosis in the chinese population.
Am J Hum Genet, 2013-01-17;92(2):177-87.
Species: Human
Sample Types: Plasma
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Human Thrombomodulin/BDCA-3 Quantikine ELISA Kit
By Ann Cullinane
on 07/18/2017
Sample Tested: Citrate Plasma
