Human Thrombospondin-1 Antibody

Detection of Human Thrombospondin‑1 by Western Blot. Western blot shows lysates of MDA-MB-231 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Thrombospondin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3074) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Thrombospondin-1 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Thrombospondin‑1 in Human Colon. Thrombospondin-1 was detected in immersion fixed paraffin-embedded sections of human colon using Goat Anti-Human Thrombospondin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3074) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human Thrombospondin‑1 by Simple Western^TM. Simple Western lane view shows lysates of MBA-MB-468 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Thrombospondin-1 at approximately 221 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Thrombospondin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3074) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Mouse Thrombospondin-1 by Western Blot Endothelial cells and TGF beta are not affected by cardiac Thbs1 overexpression.a Quantification of capillary number per mm2 of tissue from histological sections of tTA cont. and Thbs1 DTG hearts stained with isolectin B4 at 6 weeks of age. b Quantification of endothelial cell proliferation as measured by EdU incorporation co-labeled with CD31 in tTA cont. and Thbs1 DTG hearts at 6 weeks of age. c Quantification of endothelial cell apoptosis detected by TUNEL staining co-labeled with isolectin B4 in tTA cont. and Thbs1 DTG hearts at 6 weeks of age. d ELISA-based quantification of total TGF beta and e active TGF beta in protein extracts from tTA cont. or Thbs1 DTG hearts at 6 weeks of age. f Schematic diagram of WT Thbs1 domain structure and the Thbs1 delta t1 mutant lacking the Thbs1 type-1 repeat domain region. g Representative western blot analysis for Thbs1 from total protein extracts (Total) and extracellular matrix (ECM) extracts from hearts of tTA cont., Thbs1 DTG, and Thbs1 DTG delta t1 mice at 4 weeks of age. Vinculin is presented as cytosolic control. Coomassie stained (Coom.) gel is shown as loading control. h VW/BW ratio at 4 weeks of age in the indicated groups of mice. *P < 0.0001 versus tTA cont.; statistical analysis was performed using one-way ANOVA and Tukey multiple comparisons test. i Kaplan–Meier survival plot of tTA cont., Thbs1 DTG, and Thbs1 delta t1 DTG animals. *P < 0.0001 vs tTA cont. #P < 0.0001 vs Thbs1 DTG; both analyzed by two-tailed log-rank test. The same data from Fig. 2e are shown again here for tTA cont. and Thbs1 DTG mice (same strain and ages and sex ratio mix). j Representative western blots for Thbs2 and Gapdh as loading control, from heart protein extracts from tTA cont. and Thbs2 DTG mice at 8 weeks of age. k Heart weight (HW)/BW ratio, and l FS percentage at 8 weeks of age from tTA cont. and Thbs2 DTG mice. The number of biologically independent animals analyzed is indicated on each graph. Error bars are ±standard error of the mean. Source data are provided as a Source Data File. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34168130), licensed under a CC-BY license. Not internally tested by R&D Systems.