Human Thrombospondin-4 Antibody Summary
Ala22-Asn961 (Pro276Ala, Ala420Val)
Accession # P35443
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Thrombospondin‑4 by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Thrombospondin-4 Monoclonal Antibody (Catalog # MAB2390) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Thrombospondin-4 at approximately 130kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Thrombospondin-4
Thrombospondin-4 (TSP-4) is a 140 kDa calcium-binding protein that interacts with other extracellular matrix molecules and modulates the activity of various cell types. TSP‑1 and -2 constitute subgroup A and form disulfide-linked homotrimers, whereas TSP-3, -4, and -5/COMP constitute subgroup B and form pentamers (1, 2). The human TSP-4 cDNA encodes a 961 amino acid (aa) precursor that includes a 26 aa signal sequence followed by an N-terminal heparin-binding domain, a coiled-coil motif, four EGF-like repeats, seven TSP type-3 repeats (one with an RGD motif), and a TSP C-terminal domain (3). Human TSP-4 shares 93% aa sequence identity with mouse and rat TSP-4. Within the TSP type-3 repeats and the TSP C-terminal domain, human TSP-4 shares 79% aa sequence identity with TSP-3 and COMP, and 58% aa sequence identity with TSP-1 and -2. The coiled-coil motif mediates pentamer formation with COMP, either homotypically or heterotypically (3-6). TSP-4 binds a variety of matrix proteins including collagens I, II, III, V, laminin-1, fibronectin, and matrilin-2 (4). Interactions of TSP-4 with non-collagenous proteins are independent of divalent cations, while interactions with collagenous proteins are enhanced in the presence of zinc (4). TSP-4 is expressed in heart, skeletal muscle, vascular smooth muscle, and vascular endothelial cells (7-9). It accumulates at neuromuscular junctions and synapse-rich regions and is upregulated in muscle by experimental denervation (8). TSP-4 mediates the adhesion of motor and sensory neurons and promotes neurite outgrowth (8). A polymorphism of TSP-4 (A387P) is associated with early coronary artery disease (10-12). Unlike wild type TSP-4, the A387P variant does not support HUVEC attachment and spreading (9). Integrin alpha M/ beta 2 enables activated neutrophil adhesion to both the variant A387P and wild type TSP-4, although the A387P variant induces a greater release of proinflammatory molecules (13).
- Adams, J.C. and J. Lawler (2004) Int. J. Biochem. Cell Biol. 36:961.
- Stenina, O.I. et al. (2004) Int. J. Biochem. Cell Biol. 36:1013.
- Lawler, J. et al. (1995) J. Biol. Chem. 270:2809.
- Narouz-Ott, L. et al. (2000) J. Biol. Chem. 275:37110.
- Hauser, N. et al. (1995) FEBS Lett. 368:307.
- Sodersten, F. et al. (2006) Connect. Tissue Res. 47:85.
- Lawler, J. et al. (1993) J. Cell Biochem. 120:1059.
- Arber, S. and P. Caroni (1995) J. Cell Biol. 131:1083.
- Stenina, O.I. et al. (2003) Circulation 108:1514.
- Topol, E.J. et al. (2001) Circulation 104:2641.
- Wessel, J. et al. (2004) Am. Heart J. 147:905.
- Stenina, O.I. et al. (2005) FASEB J. 19:1893.
- Pluskota, E. et al. (2005) Blood 106:3970.
Product Datasheets
Citations for Human Thrombospondin-4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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The clinicopathological significance of Thrombospondin-4 expression in the tumor microenvironment of gastric cancer
Authors: K Kuroda, M Yashiro, T Sera, Y Yamamoto, Y Kushitani, A Sugimoto, S Kushiyama, S Nishimura, S Togano, T Okuno, T Tamura, T Toyokawa, H Tanaka, K Muguruma, M Ohira
PLoS ONE, 2019-11-08;14(11):e0224727.
Species: Human
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC, Western Blot -
Thrombospondin-4 reduces binding affinity of [(3)H]-gabapentin to calcium-channel ?2?-1-subunit but does not interact with ?2?-1 on the cell-surface when co-expressed
Authors: B Lana, KM Page, I Kadurin, S Ho, M Nieto-Rost, AC Dolphin
Sci Rep, 2016-04-14;6(0):24531.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
THBS4, a novel stromal molecule of diffuse-type gastric adenocarcinomas, identified by transcriptome-wide expression profiling.
Authors: Forster S, Gretschel S, Jons T, Yashiro M, Kemmner W
Mod. Pathol., 2011-06-24;24(10):1390-403.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
Thrombospondin-4 is a putative tumour-suppressor gene in colorectal cancer that exhibits age-related methylation.
Authors: Greco SA, Chia J, Inglis KJ
BMC Cancer, 2010-09-16;10(0):494.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
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We used this antibody for a sandwich ELISA in combination with pAb (AF2390)) and protein (2390-TH). This combination works very well for detecting the TSP4 in human serum and plasma
