Human THSD1 Isoform 2 Biotinylated Antibody

Catalog #: BAF5178 Datasheet / COA / SDS

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Human THSD1 Isoform 2 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human THSD1 Isoform 2 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse THSD1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human THSD1 Isoform 2
Glu25-Ile361
Accession # Q9NS62

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human THSD1 Isoform 2

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: THSD1

THSD1 (thrombospondin type 1 domain‑containing protein 1; also TMTSP) is a 90‑100 kDa type I transmembrane protein that is reminiscent of Unc5h proteins. It is expressed on embryonic endothelial and hematopoietic stem cells and may be involved in cell‑cell interactions. Mature human THSD1 is 827 amino acids (aa) in length. It contains a 388 aa extracellular domain (ECD) (aa 25‑412) and a 418 aa cytoplasmic region (aa 434‑851). The ECD possesses three Ig‑like domains
(aa 156‑316) and one TSP type‑1 domain (aa 340‑393). There is one potential alternate start site at Met380 and two splice variants, one of which shows a deletion of aa 341‑393 and a second that shows a 36 aa substitution for aa 394‑851, generating a soluble form. Over aa 25‑361, human THSD1 is 76% aa identical to mouse THSD1.

Long Name

Thrombospondin, Type I, Domain Containing 1

Entrez Gene IDs

55901 (Human); 56229 (Mouse); 364630 (Rat)

Alternate Names

thrombospondin type-1 domain-containing protein 1; thrombospondin, type I, domain 1; thrombospondin, type I, domain containing 1,4833423O18Rik; THSD1; TMTSP; TMTSPMGC74971; Transmembrane molecule with thrombospondin module; UNQ3010