Human Tie-2 Antibody Summary
Ala23-Lys745
Accession # AAA61139
Applications
under non-reducing conditions only
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data

Detection of Tie‑2 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human Tie-2 Monoclonal Antibody (Catalog # MAB3131, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tie-2
Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.
Human Tie-2 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Two ligands, angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2), which bind Tie-2 with high-affinity have been identified. Ang-2 has been reported to act as an antagonist for Ang-1. Mice engineered to overexpress Ang-2 or to lack Ang-1 or Tie-2 display similar angiogenesis defects.
- Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159.
- Takakura, N. et al. (1998) Immunity 9:677.
- Procopio, W. et al. (1999) J. Biol. Chem. 274:30196.
Product Datasheets
Citations for Human Tie-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Characteristics and potentials of stem cells derived from human degenerated nucleus pulposus: potential for regeneration of the intervertebral disc
Authors: XC Li, Y Tang, JH Wu, PS Yang, DL Wang, DK Ruan
BMC Musculoskelet Disord, 2017-06-05;18(1):242.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Resistance of acute myeloid leukemic cells to the triterpenoid CDDO-Imidazolide is associated with low caspase-8 and FADD levels.
Authors: Riccioni R, Senese M, Diverio D, Riti V, Mariani G, Boe A, LoCoco F, Foa R, Peschle C, Sporn M, Testa U
Leuk. Res., 2008-03-04;32(8):1244-58.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Expression of Tie-2 and other receptors for endothelial growth factors in acute myeloid leukemias is associated with monocytic features of leukemic blasts.
Authors: Riccioni R, Diverio D, Mariani G, Buffolino S, Riti V, Saulle E, Petrucci E, Cedrone M, Lo-Coco F, Foa R, Peschle C, Testa U
Stem Cells, 2007-04-19;25(8):1862-71.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities.
Authors: Ponte AL, Marais E, Gallay N, Langonne A, Delorme B, Herault O, Charbord P, Domenech J
Stem Cells, 2007-03-29;25(7):1737-45.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Expansion of human SCID-repopulating cells under hypoxic conditions.
Authors: Danet GH, Pan Y, Luongo JL, Bonnet DA, Simon MC
J. Clin. Invest., 2003-07-01;112(1):126-35.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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