Human Tie-2 Antibody Summary
Ala23-Lys745
Accession # Q02763
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of Human Tie‑2 by Western Blot. Western blot shows lysate of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Tie-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF313) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Tie-2 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Tie‑2 in Human Placenta. Tie-2 was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human Tie-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF313) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Tie‑2 by Simple WesternTM. Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.2 mg/mL. A specific band was detected for Tie-2 at approximately 161 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Tie-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF313) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tie-2
Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.
Human Tie-2 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Two ligands, angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2), which bind Tie-2 with high-affinity have been identified. Ang-2 has been reported to act as an antagonist for Ang-1. Mice engineered to overexpress Ang-2 or to lack Ang-1 or Tie-2 display similar angiogenesis defects.
- Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159.
- Takakura, N. et al. (1998) Immunity 9:677.
- Procopio, W. et al. (1999) J. Biol. Chem. 274:30196.
Product Datasheets
Citations for Human Tie-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Circulating Tie2-Expressing Monocytes: A Potential Biomarker for Cervical Cancer
Authors: Q Han, Q Zhang, F Ying, Z Wang, Y Zhang, L Gong, E Cai, J Qian, J Cai
OncoTargets and Therapy, 2020-09-07;13(0):8877-8885.
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SMAD4 maintains the fluid shear stress set point to protect against arterial-venous malformations
Authors: Kuheli Banerjee, Yanzhu Lin, Johannes Gahn, Julio Cordero, Purnima Gupta, Islam Mohamed et al.
Journal of Clinical Investigation
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Polydom/SVEP1 binds to Tie1 and promotes migration of lymphatic endothelial cells
Authors: Ryoko Sato-Nishiuchi, Masamichi Doiguchi, Nanami Morooka, Kiyotoshi Sekiguchi
Journal of Cell Biology
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Lymphoid Lineage gamma δ T Cells Were Successfully Generated from Human Pluripotent Stem Cells via Hemogenic Endothelium
Authors: Soo-Been Jeon, A-Reum Han, Yoo Bin Choi, Ah Reum Lee, Ji Yoon Lee
International Journal of Stem Cells
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CD34dim cells identified as pluripotent stem cell‐derived definitive hemogenic endothelium purified using bone morphogenetic protein 4
Authors: Soo‐Been Jeon, A‐Reum Han, Sunghun Lee, Seung Chan Lee, Min Ji Lee, Soon‐Jung Park et al.
Cell Proliferation
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Increased frequency of proangiogenic tunica intima endothelial kinase 2 (Tie2) expressing monocytes in individuals with type 2 diabetes mellitus
Authors: M Reijrink, J van Ark, CPH Lexis, LM Visser, ME Lodewijk, ICC van der Ho, CJ Zeebregts, H van Goor, SCA de Jager, G Pasterkamp, BHR Wolffenbut, JL Hillebrand
Cardiovascular Diabetology, 2022-05-12;21(1):72.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Aberrant stromal tissue factor localisation and mycolactone-driven vascular dysfunction, exacerbated by IL-1beta, are linked to fibrin formation in Buruli ulcer lesions
Authors: LT Hsieh, SJ Dos Santos, BS Hall, J Ogbechi, AD Loglo, FJ Salguero, MT Ruf, G Pluschke, RE Simmonds
PloS Pathogens, 2022-01-31;18(1):e1010280.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
TNF-&alpha induces endothelial-mesenchymal transition promoting stromal development of pancreatic adenocarcinoma
Authors: M Adjuto-Sac, P Soubeyran, J Garcia, S Audebert, L Camoin, M Rubis, J Roques, B Binétruy, JL Iovanna, R Tournaire
Cell Death & Disease, 2021-06-25;12(7):649.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Dynamic angiopoietin-2 assessment predicts survival and chronic course in hospitalized patients with COVID-19
Authors: Erica Villa, Rosina Critelli, Simone Lasagni, Alessandra Melegari, Angela Curatolo, Ciro Celsa et al.
Blood Advances
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Vanucizumab mode of action: Serial biomarkers in plasma, tumor, and skin-wound-healing biopsies
Authors: F Heil, G Babitzki, A Julien-Laf, CH Ooi, M Hidalgo, C Massard, M Martinez-G, C Le Tournea, M Kockx, P Gerber, S Rossomanno, O Krieter, A Lahr, N Wild, SV Harring, K Lechner
Translational Oncology, 2020-12-15;14(2):100984.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Sulfated glycans engage the Ang-Tie pathway to regulate vascular development
Authors: ME Griffin, AW Sorum, GM Miller, WA Goddard, LC Hsieh-Wils
Nat Chem Biol, 2020-10-05;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Role of the Ang2-Tie2 Axis in Vascular Damage Driven by High Glucose or Nucleoside Diphosphate Kinase B Deficiency
Authors: A Chatterjee, R Eshwaran, H Huang, D Zhao, M Schmidt, T Wieland, Y Feng
Int J Mol Sci, 2020-05-25;21(10):.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Targeting the ER&beta/Angiopoietin-2/Tie-2 signaling-mediated angiogenesis with the FDA-approved anti-estrogen Faslodex to increase the Sunitinib sensitivity in RCC
Authors: J Gu, Y Zhang, Z Han, L Gao, J Cui, Y Sun, Y Niu, B You, CP Huang, C Chang, X Wang, S Yeh
Cell Death Dis, 2020-05-14;11(5):367.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
miR-15a/-16 Inhibit Angiogenesis by Targeting the Tie2 Coding Sequence: Therapeutic Potential of a miR-15a/16 Decoy System in Limb Ischemia
Authors: Marie Besnier, Saran Shantikumar, Maryam Anwar, Parul Dixit, Aranzazu Chamorro-Jorganes, Walid Sweaad et al.
Molecular Therapy - Nucleic Acids
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Weibel-Palade Bodies Orchestrate Pericytes During Angiogenesis
Authors: Mélissande Cossutta, Marie Darche, Gilles Carpentier, Claire Houppe, Matteo Ponzo, Fabio Raineri et al.
Arteriosclerosis, Thrombosis, and Vascular Biology
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Tie2 activation promotes choriocapillary regeneration for alleviating neovascular age-related macular degeneration
Authors: J Kim, JR Park, J Choi, I Park, Y Hwang, H Bae, Y Kim, W Choi, JM Yang, S Han, TY Chung, P Kim, Y Kubota, HG Augustin, WY Oh, GY Koh
Sci Adv, 2019-02-13;5(2):eaau6732.
Species: Human, Mouse
Sample Types: Cell Culture Supernates, Whole Cells, Whole Tissue
Applications: ICC, IHC-Fr, Immunoprecipitation -
An acellular bioresorbable ultra-purified alginate gel promotes intervertebral disc repair: A preclinical proof-of-concept study
Authors: T Tsujimoto, H Sudo, M Todoh, K Yamada, K Iwasaki, T Ohnishi, N Hirohama, T Nonoyama, D Ukeba, K Ura, YM Ito, N Iwasaki
EBioMedicine, 2018-10-30;0(0):.
Species: Rabbit
Sample Types: Whole Cells
Applications: ICC -
Structural basis of Tie2 activation and Tie2/Tie1 heterodimerization
Authors: VM Leppänen, P Saharinen, K Alitalo
Proc. Natl. Acad. Sci. U.S.A., 2017-04-10;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Endothelial destabilization by angiopoietin-2 via integrin beta1 activation.
Authors: Hakanpaa L, Sipila T, Leppanen V, Gautam P, Nurmi H, Jacquemet G, Eklund L, Ivaska J, Alitalo K, Saharinen P
Nat Commun, 2015-01-30;6(0):5962.
Species: Human
Sample Types: Whole Cells
Applications: ICC, IHC -
Inhibition of Tyrosine Kinase Receptor Tie2 Reverts HCV-Induced Hepatic Stellate Cell Activation
Authors: Samuel Martín-Vílchez, Yolanda Rodríguez-Muñoz, Rosario López-Rodríguez, Ángel Hernández-Bartolomé, María Jesús Borque-Iñurrita, Francisca Molina-Jiménez et al.
PLoS ONE
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slanDCs selectively accumulate in carcinoma-draining lymph nodes and marginate metastatic cells.
Authors: Vermi W, Micheletti A, Lonardi S, Costantini C, Calzetti F, Nascimbeni R, Bugatti M, Codazzi M, Pinter P, Schakel K, Tamassia N, Cassatella M
Nat Commun, 2014-01-01;5(0):3029.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Neurovascular Signals Suggest a Propagation Mechanism for Endogenous Stem Cell Activation Along Blood Vessels
Authors: Jimmy Masjkur, Maria Adele Adele Rueger, Stefan R R. Bornstein, Ron McKay, Andreas Androutsellis-Theotokis
CNS & Neurological Disorders - Drug Targets
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Chromatin structure of two genomic sites for targeted transgene integration in induced pluripotent stem cells and hematopoietic stem cells.
Authors: van Rensburg, R, Beyer, I, Yao, X-Y, Wang, H, Denisenko, O, Li, Z-Y, Russell, D W, Miller, D G, Gregory, P, Holmes, M, Bomsztyk, K, Lieber, A
Gene Ther, 2012-03-22;20(2):201-14.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Exhaustion of nucleus pulposus progenitor cells with ageing and degeneration of the intervertebral disc.
Authors: Sakai, Daisuke, Nakamura, Yoshihik, Nakai, Tomoko, Mishima, Taishi, Kato, Shunichi, Grad, Sibylle, Alini, Mauro, Risbud, Makarand, Chan, Danny, Cheah, Kathryn, Yamamura, Ken-ichi, Masuda, Koichi, Okano, Hideyuki, Ando, Kiyoshi, Mochida, Joji
Nat Commun, 2012-01-01;3(0):1264.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Opposing effects of the angiopoietins on the thrombin-induced permeability of human pulmonary microvascular endothelial cells.
Authors: van der Heijden M, van Nieuw Amerongen GP, van Bezu J, Paul MA, Groeneveld AB, van Hinsbergh VW
PLoS ONE, 2011-08-15;6(8):e23448.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot -
Tumour vascularization via endothelial differentiation of glioblastoma stem-like cells.
Authors: Ricci-Vitiani L, Pallini R, Biffoni M, Todaro M, Invernici G, Cenci T, Maira G, Parati EA, Stassi G, Larocca LM, De Maria R
Nature, 2010-11-21;468(7325):824-8.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, IHC-P -
Angiopoietin-2 interferes with anti-VEGFR2- induced vessel normalization and survival benefit in mice bearing gliomas
Authors: Sung-Suk Chae, Walid S. Kamoun, Christian T. Farrar, Nathaniel D. Kirkpatrick, Elisabeth Niemeyer, Annemarie M.A. de Graaf et al.
Clinical Cancer Research
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Cholera Toxin Regulates a Signaling Pathway Critical for the Expansion of Neural Stem Cell Cultures from the Fetal and Adult Rodent Brains
Authors: Andreas Androutsellis-Theotokis, Stuart Walbridge, Deric M. Park, Russell R. Lonser, Ronald D. G. McKay
PLoS ONE
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VEGF-DdeltaNdeltaC mediated angiogenesis in skeletal muscles of diabetic WHHL rabbits.
Authors: Roy H, Bhardwaj S, Babu M, Lahteenvuo JE, Yla-Herttuala S
Eur. J. Clin. Invest., 2010-05-01;40(5):422-32.
Species: Rabbit
Sample Types: Whole Tissue
Applications: IHC-P -
The angiogenic factor angiopoietin-1 is a proneurogenic peptide on subventricular zone stem/progenitor cells.
Authors: Rosa AI, Goncalves J, Cortes L, Bernardino L, Malva JO, Agasse F
J. Neurosci., 2010-03-31;30(13):4573-84.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells, Whole Tissue
Applications: ICC, IHC, Neutralization, Western Blot -
Expression of VEGF receptors VEFGR-1 and VEGFR-2, angiopoietin receptors Tie-1 and Tie-2 in chorionic villi tree during early pregnancy
Authors: Ramazan Demir
Folia Histochemica et Cytobiologica
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Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation.
Authors: Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O
Eur. J. Haematol., 2009-11-17;84(3):239-51.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Interaction with human stromal cells enhances CXCR4 expression and engraftment of cord blood Lin(-)CD34(-) cells.
Authors: Kobune M, Kawano Y, Takahashi S, Takada K, Murase K, Iyama S, Sato T, Takimoto R, Niitsu Y, Kato J
Exp. Hematol., 2008-06-17;36(9):1121-31.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Angiopoietins assemble distinct Tie2 signalling complexes in endothelial cell-cell and cell-matrix contacts.
Authors: Saharinen P, Eklund L, Miettinen J, Wirkkala R, Anisimov A, Winderlich M, Nottebaum A, Vestweber D, Deutsch U, Koh GY, Olsen BR, Alitalo K
Nat. Cell Biol., 2008-04-20;10(5):527-37.
Species: Human
Sample Types: Whole Cells
Applications: ICC, Western Blot -
Regulated proteolytic processing of Tie1 modulates ligand responsiveness of the receptor-tyrosine kinase Tie2.
Authors: Marron MB, Singh H, Tahir TA, Kavumkal J, Kim HZ, Koh GY, Brindle NP
J. Biol. Chem., 2007-08-29;282(42):30509-17.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Interaction between Tie receptors modulates angiogenic activity of angiopoietin2 in endothelial progenitor cells.
Authors: Kim KL, Shin IS, Kim JM, Choi JH, Byun J, Jeon ES, Suh W, Kim DK
Cardiovasc. Res., 2006-08-07;72(3):394-402.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Immunoprecipitation, Neutralization, Western Blot -
Enhancement of angiogenic effectors through hypoxia-inducible factor in preterm primate lung in vivo.
Authors: Asikainen TM, Waleh NS, Schneider BK, Clyman RI, White CW
Am. J. Physiol. Lung Cell Mol. Physiol., 2006-05-05;291(4):L588-95.
Species: Primate - Papio anubis (Olive Baboon)
Sample Types: Tissue Homogenates
Applications: Western Blot -
Spatial and temporal distribution of Tie-1 and Tie-2 during very early development of the human placenta.
Authors: Kayisli UA, Cayli S, Seval Y, Tertemiz F, Huppertz B, Demir R
Placenta, 2005-07-18;27(6):648-59.
Species: Human
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-P, Western Blot -
Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells and explants of primate lung.
Authors: Asikainen TM, Schneider BK, Waleh NS, Clyman RI, Ho WB, Flippin LA, Gunzler V, White CW
Proc. Natl. Acad. Sci. U.S.A., 2005-07-11;102(29):10212-7.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot -
Functional analysis of a mutant form of the receptor tyrosine kinase Tie2 causing venous malformations.
Authors: Morris PN, Dunmore BJ, Tadros A, Marchuk DA, Darland DC, D'Amore PA, Brindle NP
J. Mol. Med., 2004-10-29;83(1):58-63.
Species: Human
Sample Types: Cell Lysates, Whole Cells, Whole Tissue
Applications: ICC, IHC, Western Blot -
Proangiogenic properties of human myeloma cells: production of angiopoietin-1 and its potential relationship to myeloma-induced angiogenesis.
Authors: Giuliani N, Colla S, Lazzaretti M, Sala R, Roti G, Mancini C, Bonomini S, Lunghi P, Hojden M, Genestreti G, Svaldi M, Coser P, Fattori PP, Sammarelli G, Gazzola GC, Almici C, Caramatti C, Mangoni L, Rizzoli V
Blood, 2003-03-20;102(2):638-45.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Evidence for heterotypic interaction between the receptor tyrosine kinases TIE-1 and TIE-2.
Authors: Marron MB, Hughes DP, Edge MD, Forder CL, Brindle NP
J. Biol. Chem., 2000-12-15;275(50):39741-6.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Structural insights into the clustering and activation of Tie2 receptor mediated by Tie2 agonistic antibody
Authors: G Jo, J Bae, HJ Hong, AR Han, DK Kim, SP Hong, JA Kim, S Lee, GY Koh, HM Kim
Nature Communications, 2021-11-01;12(1):6287.
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Presence of sst5TMD4, a truncated splice variant of the somatostatin receptor subtype 5, is associated to features of increased aggressiveness in pancreatic neuroendocrine tumors.
Authors: Sampedro-Nunez M, Luque RM, Ramos-Levi AM et al.
Oncotarget
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