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Human Tie-2 ELISA Kit - Quantikine

Catalog #: DTE200
27 Citations Datasheet / COA / SDS
Newer Version Available: DY5159
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DTE200 has been discontinued and is replaced by DY5159.

Human Tie-2 ELISA Standard Curve
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Product Details
Procedure
Citations (27)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human Tie-2 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
0.066 ng/mL
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human Tie-2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Tie-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Tie-2 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human Tie-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Tie-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Tie-2.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (ng/mL) 0.76 2.57 5.14 0.82 2.65 5.15
Standard Deviation 0.04 0.11 0.26 0.07 0.17 0.27
CV% 5.3 4.3 5.1 8.5 6.4 5.2

Recovery

The recovery of Tie-2 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 91 85-101

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Tie-2 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Tie-2 ELISA Linearity

Scientific Data

N/A Human Tie-2 ELISA Standard Curve View Larger

Human Tie-2 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Tie-2

Tie-2, also known as Tek, is a transmembrane receptor tyrosine kinase (RTK) that functions as a receptor for Angiopoietin family proteins. It is expressed by embryonic and adult endothelial cells, hematopoietic stem cells, and a circulating population of proangiogenic monocytes. Tie-2 signaling is activated by Angiopoietins-1 and -4, while it can be either activated or inhibited by Angiopoietins-2 and -3. Tie-2 plays an important role in maintaining vascular integrity by mediating endothelial cell-smooth muscle cell communication and inhibiting endothelial cell apoptosis. It is also required for embryonic development of the endocardium. In addition, Tie-2 signaling mediates the quiescence of bone marrow stem cells in response to osteoblast-produced Angiopoietin-1. This quiescence is critical for maintaining an ongoing hematopoietic capability in the bone marrow.

Long Name:
Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2
Entrez Gene IDs:
7010 (Human); 21687 (Mouse); 89804 (Rat); 396729 (Porcine); 403714 (Canine); 102122204 (Cynomolgus Monkey); 30747 (Zebrafish)
Alternate Names:
angiopoietin-1 receptor; CD202b antigen; CD202b; EC 2.7.10; EC 2.7.10.1; hTIE2; p140 TEK; soluble TIE2 variant 1; soluble TIE2 variant 2; TEK tyrosine kinase, endothelial; TEK; Tie2; Tie-2; TIE2CD202b; Tunica interna endothelial cell kinase; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2; venous malformations, multiple cutaneous and mucosal; VMCM; VMCM1
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 100 µL Standard, Control, or Sample
  4.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human Tie-2 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

27 Citations: Showing 1 - 10
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  1. Fluid bolus increases plasma hyaluronan concentration compared to follow-up strategy without a bolus in oliguric intensive care unit patients
    Authors: Serlo, M;Inkinen, N;Lakkisto, P;Valkonen, M;Pulkkinen, A;Selander, T;Pettilä, V;Vaara, ST;
    Scientific reports
    Species: Human
    Sample Types: Plasma
  2. Circulating Soluble EPCR Levels Are Reduced in Patients with Ischemic Peripheral Artery Disease and Associated with Markers of Endothelial and Vascular Function
    Authors: Krug, J;Bochenek, ML;Gogiraju, R;Laubert-Reh, D;Lackner, KJ;Münzel, T;Wild, PS;Espinola-Klein, C;Schäfer, K;
    Biomedicines
    Species: Human
    Sample Types: Plasma
  3. Serum angiopoietin-1 concentration does not distinguish patients with ischaemic stroke from those presenting to hospital with ischaemic stroke mimics
    Authors: JV Moxon, AK Kraeuter, J Phie, S Juliano, G Anderson, G Standley, C Sealey, RP White, J Golledge
    BMC cardiovascular disorders, 2022-11-04;22(1):462.
  4. Cholecalciferol supplementation and angiogenic markers in chronic kidney disease
    Authors: J Kaur, K Kamboj, AK Yadav, P Kaur, V Kumar, V Jha
    PLoS ONE, 2022-06-03;17(6):e0268946.
    Species: Human
    Sample Types: Serum
  5. Microcirculatory perfusion disturbances following cardiac surgery with cardiopulmonary bypass are associated with in vitro endothelial hyperpermeability and increased angiopoietin-2 levels
    Authors: NAM Dekker, ALI van Leeuwe, WWJ van Strien, J Majolée, R Szulcek, ABA Vonk, PL Hordijk, C Boer, CE van den Br
    Crit Care, 2019-04-11;23(1):117.
    Species: Human
    Sample Types: Plasma
  6. Different patterns of inflammatory and angiogenic factors are associated with peritoneal small solute transport and peritoneal protein clearance in peritoneal dialysis patients
    Authors: Y Shi, H Yan, J Yuan, H Zhang, J Huang, Z Ni, J Qian, W Fang
    BMC Nephrol, 2018-05-23;19(1):119.
    Species: Human
    Sample Types: Serum
  7. Tie2 as a novel key factor of microangiopathy in systemic sclerosis
    Authors: F Moritz, J Schniering, JHW Distler, RE Gay, S Gay, O Distler, B Maurer
    Arthritis Res. Ther., 2017-05-25;19(1):105.
    Species: Human
    Sample Types: Serum
  8. Angiopoietin-2 and soluble Tie-2 receptor plasma levels in children with obstructive sleep apnea and obesity
    Authors: D Gozal, A Khalyfa, Z Qiao, DL Smith, MF Philby, D Koren, L Kheirandis
    Obesity (Silver Spring), 2017-05-05;0(0):.
    Species: Human
    Sample Types: Plasma
  9. TIE2-mediated tyrosine phosphorylation of H4 regulates DNA damage response by recruiting ABL1
    Sci Adv, 2016-04-01;2(4):e1501290.
    Species: Human
    Sample Types: Cell Lysates
  10. Angiopoietin/Tie2 Dysbalance Is Associated with Acute Kidney Injury after Cardiac Surgery Assisted by Cardiopulmonary Bypass.
    Authors: Jongman R, van Klarenbosch J, Molema G, Zijlstra J, de Vries A, van Meurs M
    PLoS ONE, 2015-08-26;10(8):e0136205.
    Species: Human
    Sample Types: Plasma
  11. Clinical outcome, proteome kinetics and angiogenic factors in serum after thermoablation of colorectal liver metastases.
    Authors: Wertenbroek, Marieke, Schepers, Marianne, Kamminga-Rasker, Hannetta, Bottema, Jan T, Muller Kobold, Anneke C, Roelofsen, Han, de Jong, Koert P
    BMC Cancer, 2013-05-30;13(1):266.
    Species: Human
    Sample Types: Serum
  12. Circulating angiopoietin-2 and soluble Tie-2 in type 2 diabetes mellitus: a cross-sectional study.
    Authors: Rasul S, Reiter MH, Ilhan A, Lampichler K, Wagner L, Kautzky-Willer A
    Cardiovasc Diabetol, 2011-06-23;10(0):55.
    Species: Human
    Sample Types: Plasma
  13. Shedding of the endothelial receptor tyrosine kinase Tie2 correlates with leukemic blast burden and outcome after allogeneic hematopoietic stem cell transplantation for AML.
    Authors: Koenecke C, Kumpers P, Lukasz A, Dammann E, Verhagen W, Gohring G, Buchholz S, Krauter J, Eder M, Schlegelberger B, Ganser A
    Ann. Hematol., 2010-05-01;89(5):459-67.
    Species: Human
    Sample Types: Serum
  14. Defective angiogenesis in hypoplastic human fetal lungs correlates with nitric oxide synthase deficiency that occurs despite enhanced angiopoietin-2 and VEGF.
    Authors: Boucherat O, Franco-Montoya ML, Delacourt C, Martinovic J, Masse V, Elie C, Thebaud B, Benachi A, Bourbon JR
    Am. J. Physiol. Lung Cell Mol. Physiol., 2010-03-26;298(6):L849-56.
    Species: Human
    Sample Types: Tissue Homogenates
  15. Changes in vascular endothelial growth factor, angiopoietins, and Tie-2 levels with G-CSF stimulation in healthy donors.
    Authors: Serefhanoglu S, Goker H, Buyukasik Y, Turgut M, Sayinalp N, Haznedaroglu IC, Aksu S, Akman U, Ozcebe OI
    Ann. Hematol., 2008-12-11;88(7):667-71.
    Species: Human
    Sample Types: Plasma
  16. The angiopoietin/Tie-2 system regulates pericyte survival and recruitment in diabetic retinopathy.
    Authors: Cai J, Kehoe O, Smith GM, Hykin P, Boulton ME
    Invest. Ophthalmol. Vis. Sci., 2008-05-01;49(5):2163-71.
    Species: Bovine
    Sample Types: Cell Lysates
  17. Marrow angiogenesis-associated factors as prognostic biomarkers in patients with acute myelogenous leukaemia.
    Authors: Lee CY, Tien HF, Hu CY, Chou WC, Lin LI
    Br. J. Cancer, 2007-09-11;97(7):877-82.
    Species: Human
    Sample Types: Plasma
  18. Angiopoietin 1 (Ang-1), angiopoietin 2 (Ang-2) and Tie-2 (a receptor tyrosine kinase) concentrations in peripheral blood of patients with thyroid cancers.
    Authors: Niedzwiecki S, Stepien T, Kopec K, Kuzdak K, Komorowski J, Krupinski R, Stepien H
    Cytokine, 2007-03-19;36(5):291-5.
    Species: Human
    Sample Types: Serum
  19. Significant decrease in angiopoietin-1 and angiopoietin-2 after radical prostatectomy in prostate cancer patients.
    Authors: Caine GJ, Ryan P, Lip GY, Blann AD
    Cancer Lett., 2007-01-19;251(2):296-301.
    Species: Human
    Sample Types: Plasma
  20. Differential levels of soluble angiopoietin-2 and Tie-2 in patients with haematological malignancies.
    Authors: Quartarone E, Alonci A, Allegra A, Bellomo G, Calabro L, D'Angelo A, Del Fabro V, Grasso A, Cincotta M, Musolino C
    Eur. J. Haematol., 2006-09-15;77(6):480-5.
    Species: Human
    Sample Types: Serum
  21. Circulating levels of total angiopoietin-2 and the soluble Tie-2 receptor in women during ovarian stimulation and early gestation.
    Authors: Molskness TA, Stouffer RL, Burry KA, Gorrill MJ, Lee DM, Patton PE
    Fertil. Steril., 2006-09-14;86(5):1531-3.
  22. Changes in plasma vascular endothelial growth factor, angiopoietins, and their receptors following surgery for breast cancer.
    Authors: Caine GJ, Stonelake PS, Lip GY, Blann AD
    Cancer Lett., 2006-08-07;248(1):131-6.
    Species: Human
    Sample Types: Plasma
  23. Differential expression of angiopoietin-1, angiopoietin-2, and Tie receptors in placentas from pregnancies complicated by placenta accreta.
    Authors: Tseng JJ, Hsu SL, Ho ES, Hsieh YT, Wen MC, Chou MM
    Am. J. Obstet. Gynecol., 2006-02-01;194(2):564-71.
    Species: Human
    Sample Types: Tissue Homogenates
  24. Potential role of soluble angiopoietin-2 and Tie-2 in patients with inflammatory bowel disease.
    Authors: Koutroubakis IE, Xidakis C, Karmiris K, Sfiridaki A, Kandidaki E, Kouroumalis EA
    Eur. J. Clin. Invest., 2006-02-01;36(2):127-32.
    Species: Human
    Sample Types: Serum
  25. Multiple roles for the receptor tyrosine kinase axl in tumor formation.
    Authors: Holland SJ, Powell MJ, Franci C, Chan EW, Friera AM, Atchison RE, McLaughlin J, Swift SE, Pali ES, Yam G, Wong S, Lasaga J, Shen MR, Yu S, Xu W, Hitoshi Y, Bogenberger J, Nör JE, Payan DG, Lorens JB
    Cancer Res., 2005-10-15;65(20):9294-303.
    Species: Human
    Sample Types: Cell Lysates
  26. Phase I clinical and pharmacokinetic study of PTK/ZK, a multiple VEGF receptor inhibitor, in patients with liver metastases from solid tumours.
    Authors: Mross K, Drevs J, Muller M, Medinger M, Marme D, Hennig J, Morgan B, Lebwohl D, Masson E, Ho YY, Gunther C, Laurent D, Unger C
    Eur. J. Cancer, 2005-06-01;41(9):1291-9.
    Species: Human
    Sample Types: Serum
  27. Bi-directional cell contact-dependent regulation of gene expression between endothelial cells and osteoblasts in a three-dimensional spheroidal coculture model.
    Authors: Stahl A, Wenger A, Weber H, Stark GB, Augustin HG, Finkenzeller G
    Biochem. Biophys. Res. Commun., 2004-09-17;322(2):684-92.
    Species: Human
    Sample Types: Cell Culture Supernates

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