Human Tiki2/TRABD2B Antibody Summary
Accession # A6NFA1
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Tiki2/TRABD2B by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human Tiki2/TRABD2B. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Tiki2/TRABD2B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1600) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Tiki2/TRABD2B at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Tiki2/TRABD2B in HEK293 Human Cell Line Transfected with Human Tiki2/TRABD2B by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either human Tiki2/ TRABD2B (filled histogram) or irrelevant transfectants (open histogram) was stained with Sheep Anti-Human Tiki2/TRABD2B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1600), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tiki2/TRABD2B
TIKI2, encoded by the TRABD2B gene, is a 517 aminoacid metalloproteinase that acts as a negative regulator of Wnt signaling by mediating the cleavage of N-terminal residues in many of Wnt proteins. Tiki matalloproteins are dependent on Mn2+/Co2+ and are inhibited by divalent metal chelators like EDTA. The other known Tiki protein in human, Tiki1, is required for head formation via Wnt cleavage-oxidation and inactivation, but lacks known metalloprotease motifs. TIKI2 is upregulated in renal cell carcinoma and it appears to suppress growth of osteosarcoma by targeting the canonical Wnt pathway
Product Datasheets
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