Human TIM-3 Antibody

Catalog #: MAB2365R Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB2365R-100
MAB2365R-SP

Detection of TIM‑3 in Human PBMCs by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TIM-3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TIM-3 in direct ELISAs.

Source

Recombinant Monoclonal Rat IgG_2A Clone # 344823R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TIM‑3
Ser22-Arg200
Accession # Q8TDQ0.2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of TIM‑3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Rat Anti-Human TIM-3 Monoclonal Antibody (Catalog # MAB2365R) or (B) Rat IgG2A isotype control antibody (Catalog # MAB006), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B) and Mouse anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-3

TIM-3 (T cell Immunoglobulin and Mucin domain-3) is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk (1-3). Mature human TIM-3 consists of a 181 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail (4). An alternately spliced isoform is truncated following a short substitution after the Ig-like domain. Within the ECD, human TIM-3 shares 58% aa sequence identity with mouse and rat TIM-3. TIM-3 is expressed on the surface of effector T cells (CD4⁺ Th1 and CD8⁺ Tc1) but not on helper T cells (CD4⁺ Th2 and CD8⁺ Tc2) (4, 5). In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types. The Ig domain of TIM-3 interacts with a ligand on resting but not activated Th1 and Th2 cells (5, 6). The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9. This induces TIM-3 Tyr phosphorylation and proapoptotic signaling (7). TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation and cytotoxicity (5, 6, 8), regulatory T cell development (5), and increases in macrophage and neutrophil infiltration into sites of inflammation (9).

References

Anderson, A.C. and D.E. Anderson (2006) Curr. Opin. Immunol. 18:665.
Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362.
Monney, L. et al. (2002) Nature 415:536.
Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
Koguchi, K. et al. (2006) J. Exp. Med. 203:1413.
Frisancho-Kiss, S. et al. (2006) J. Immunol. 176:6411.

Long Name

T Cell Immunoglobulin Mucin-3

Entrez Gene IDs

84868 (Human); 171285 (Mouse); 363578 (Rat); 479318 (Canine); 102141722 (Cynomolgus Monkey)

Alternate Names

CD366; FLJ14428; HAVCR2; HAVcr-2; hepatitis A virus cellular receptor 2; kidney injury molecule-3; KIM-3; SPTCL; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain-containing protein 3; TIM 3; TIM3 T-cell membrane protein 3; TIM3; TIM-3; TIMD3; TIMD-3; TIMD3KIM-3