Human TIM-3 APC-conjugated Antibody
Human TIM-3 APC-conjugated Antibody Summary
Ser22-Arg200
Accession # Q8TDQ0.2
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of TIM‑3 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Rat Anti-Human TIM‑3 APC‑conjugated Monoclonal Antibody (Catalog # FAB2365A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Detection of Human TIM-3 by Flow Cytometry Antigen Expression in AML and Normal Hematopoiesis. Antigen expression on primary AML samples at initial diagnosis and healthy donor-derived bone marrow cell populations (HD BM) as indicated in legend. a Representative primary AML sample and healthy donor-derived bone marrow sample. Histograms indicate fluorescence intensity. Numbers indicate MFI ratio. b Antigen expression in AML and normal hematopoiesis, shown as MFI ratio. Dots indicate measured samples. Violin plots illustrate distribution of antigen expression for each analyzed cell population. Black dotted line indicates MFI ratio of 1.5 as cutoff for positivity. c Summary of antigen expression levels (median MFI ratio) Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29946192), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TIM-3
TIM-3 (T cell Immunoglobulin and Mucin domain-3) is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk (1-3). Mature human TIM-3 consists of a 181 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail (4). An alternately spliced isoform is truncated following a short substitution after the Ig-like domain. Within the ECD, human TIM-3 shares 58% aa sequence identity with mouse and rat TIM-3. TIM-3 is expressed on the surface of effector T cells (CD4+ Th1 and CD8+ Tc1) but not on helper T cells (CD4+ Th2 and CD8+ Tc2) (4, 5). In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types. The Ig domain of TIM-3 interacts with a ligand on resting but not activated Th1 and Th2 cells (5, 6). The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9. This induces TIM-3 Tyr phosphorylation and proapoptotic signaling (7). TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation and cytotoxicity (5, 6, 8), regulatory T cell development (5), and increases in macrophage and neutrophil infiltration into sites of inflammation (9).
- Anderson, A.C. and D.E. Anderson (2006) Curr. Opin. Immunol. 18:665.
- Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
- Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362.
- Monney, L. et al. (2002) Nature 415:536.
- Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
- Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
- Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
- Koguchi, K. et al. (2006) J. Exp. Med. 203:1413.
- Frisancho-Kiss, S. et al. (2006) J. Immunol. 176:6411.
Product Datasheets
Citations for Human TIM-3 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
15
Citations: Showing 1 - 10
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Influence of Galectin-9 Treatment on the Phenotype and Function of NK-92MI Cells in the Presence of Different Serum Supplements
Authors: Matyas Meggyes, David U Nagy, Timea Balassa, Krisztina Godony, Agnes Peterfalvi, Laszlo Szereday et al.
Biomolecules
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Efficient lentiviral transduction method to gene modify cord blood CD8+ T cells for cancer therapy applications
Authors: Vania Lo Presti, Annelisa M. Cornel, Maud Plantinga, Ester Dünnebach, Jurgen Kuball, Jaap Jan Boelens et al.
Molecular Therapy - Methods & Clinical Development
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Nivolumab plus ipilimumab in advanced salivary gland cancer: a phase 2 trial
Authors: Vos, JL;Burman, B;Jain, S;Fitzgerald, CWR;Sherman, EJ;Dunn, LA;Fetten, JV;Michel, LS;Kriplani, A;Ng, KK;Eng, J;Tchekmedyian, V;Haque, S;Katabi, N;Kuo, F;Han, CY;Nadeem, Z;Yang, W;Makarov, V;Srivastava, RM;Ostrovnaya, I;Prasad, M;Zuur, CL;Riaz, N;Pfister, DG;Klebanoff, CA;Chan, TA;Ho, AL;Morris, LGT;
Nature medicine
Species: Human
Sample Types: Whole Cell
Applications: Flow Cytometry -
Phenotype and Reactivity of Lymphocytes Expanded from Benign Prostate Hyperplasic Tissues and Prostate Cancer
Authors: Ahmed, R;Lozano, LE;Anastasio, A;Lofek, S;Mastelic-Gavillet, B;Navarro Rodrigo, B;Nguyen, S;Dartiguenave, F;Rodrigues-Dias, SC;Cesson, V;Valério, M;Roth, B;Kandalaft, LE;Redchenko, I;Hill, AVS;Harari, A;Romero, P;Derré, L;Viganó, S;
Cancers
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
CD226 opposes TIGIT to disrupt Tregs in melanoma
Authors: J Fourcade, Z Sun, JM Chauvin, M Ka, D Davar, O Pagliano, H Wang, S Saada, C Menna, R Amin, C Sander, JM Kirkwood, AJ Korman, HM Zarour
JCI Insight, 2018-07-26;3(14):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Antitumor Activity Associated with Prolonged Persistence of Adoptively Transferred NY-ESO-1c259T cells in Synovial Sarcoma
Authors: SP D'Angelo, L Melchiori, MS Merchant, DB Bernstein, J Glod, RN Kaplan, SA Grupp, WD Tap, K Chagin, GK Binder, S Basu, DE Lowther, R Wang, N Bath, A Tipping, G Betts, I Ramachandr, JM Navenot, H Zhang, DK Wells, E Van Winkle, G Kari, T Trivedi, T Holdich, LN Pandite, R Amado, CL Mackall
Cancer Discov, 2018-06-11;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
T-cell invigoration to tumour burden ratio associated with anti-PD-1 response
Authors: AC Huang, MA Postow, RJ Orlowski, R Mick, B Bengsch, S Manne, W Xu, S Harmon, JR Giles, B Wenz, M Adamow, D Kuk, KS Panageas, C Carrera, P Wong, F Quagliarel, B Wubbenhors, K D'Andrea, KE Pauken, RS Herati, RP Staupe, JM Schenkel, S McGettigan, S Kothari, SM George, RH Vonderheid, RK Amaravadi, GC Karakousis, LM Schuchter, X Xu, KL Nathanson, JD Wolchok, TC Gangadhar, EJ Wherry
Nature, 2017-04-10;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Circulating T lymphocyte subsets, cytokines, and immune checkpoint inhibitors in patients with bipolar II or major depression: a preliminary study
Authors: W Wu, YL Zheng, LP Tian, JB Lai, CC Hu, P Zhang, JK Chen, JB Hu, ML Huang, N Wei, WJ Xu, WH Zhou, SJ Lu, J Lu, HL Qi, DD Wang, XY Zhou, JF Duan, Y Xu, SH Hu
Sci Rep, 2017-01-11;7(0):40530.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
TIM-3/Gal-9 interaction induces IFNgamma-dependent IDO1 expression in acute myeloid leukemia blast cells.
Authors: Folgiero V, Cifaldi L, Li Pira G, Goffredo B, Vinti L, Locatelli F
J Hematol Oncol, 2015-04-16;8(0):36.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Inhibitory phenotype of HBV-specific CD4+ T-cells is characterized by high PD-1 expression but absent coregulation of multiple inhibitory molecules.
Authors: Raziorrouh B, Heeg M, Kurktschiev P, Schraut W, Zachoval R, Wendtner C, Wachtler M, Spannagl M, Denk G, Ulsenheimer A, Bengsch B, Pircher H, Diepolder H, Gruner N, Jung M
PLoS ONE, 2014-08-21;9(8):e105703.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Peripheral blood TIM-3 positive NK and CD8+ T cells throughout pregnancy: TIM-3/galectin-9 interaction and its possible role during pregnancy.
Authors: Meggyes, Matyas, Miko, Eva, Polgar, Beata, Bogar, Barbara, Farkas, Balint, Illes, Zsolt, Szereday, Laszlo
PLoS ONE, 2014-03-20;9(3):e92371.
Species: Human
Sample Types:
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Differential regulation of interleukin-12 (IL-12)/IL-23 by Tim-3 drives T(H)17 cell development during hepatitis C virus infection.
Authors: Wang, Jia M, Shi, Lei, Ma, Cheng J, Ji, Xiao J, Ying, Ruo S, Wu, Xiao Y, Wang, Ke S, Li, Guangyu, Moorman, Jonathan, Yao, Zhi Q
J Virol, 2013-02-06;87(8):4372-83.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Blockade of Tim-3 signaling restores the virus-specific CD8+ T-cell response in patients with chronic hepatitis B.
Authors: Wu W, Shi Y, Li S, Zhang Y, Liu Y, Wu Y, Chen Z
Eur. J. Immunol., 2012-05-01;42(5):1180-91.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Tim-3 negatively regulates IL-12 expression by monocytes in HCV infection.
Authors: Zhang Y, Ma CJ, Wang JM, Ji XJ, Wu XY, Jia ZS, Moorman JP, Yao ZQ
PLoS ONE, 2011-05-26;6(5):e19664.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
PEGylated IL-10 (Pegilodecakin) Induces Systemic Immune Activation, CD8+ T Cell Invigoration and Polyclonal T Cell Expansion in Cancer Patients
Authors: Aung Naing, Jeffrey R. Infante, Kyriakos P. Papadopoulos, Ivan H. Chan, Cong Shen, Navneet P. Ratti et al.
Cancer Cell
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Human PBMCs were stimulated with anti-human CD3(1ug/ml) for 48hrs and stained with Isotype and test antibodies. The results have been consistent and reliable.
