Human TIM-4 PE-conjugated Antibody

Catalog #: FAB2929P Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB2929P-025

Detection of TIM‑4 in HEK Human Cell Line Transfected with Human TIM-4 and eGFP by Flow Cytometry.

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Human TIM-4 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human TIM-4 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 921832

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TIM-4
Glu25-Leu315
Accession # Q96H15

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of TIM‑4 in HEK Human Cell Line Transfected with Human TIM-4 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human TIM-4 and eGFP was stained with and either (A) Mouse Anti-Human TIM-4 PE-conjugated Monoclonal Antibody (Catalog # FAB2929P) or (B) Mouse IgG₁Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: TIM-4

TIM-4 (T cell; immunoglobulin; mucin-4), also known as SMUCKLER, is a 60 kDa member of the TIM family of immune regulating proteins. TIMs are type I transmembrane proteins with one Ig-like V domain and one Ser/Thr-rich mucin domain (1 - 3). The human TIM-4 cDNA encodes a 378 amino acid (aa) precursor that includes a 24 aa signal sequence, a 290 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 43 aa cytoplasmic tail (4). Structurally, TIM-4 is distinguished from other TIMs by the presence of an RGD motif in its Ig domain and the lack of a site for tyrosine phosphorylation in its cytoplasmic tail. The mucin domain in TIM-4 is larger than in TIM-1 or TIM-3. Within the ECD, human TIM-4 shares 35% and 23% aa sequence identity with TIM-1 and TIM-3, respectively. A TIM-2 ortholog has not been identified in human. The ECD of human TIM-4 shares 45% aa sequence identity with that of mouse and rat TIM-4. TIM-4 is expressed by macrophages and mature dendritic cells but not by lymphocytes (4, 5). TIM-4 binds specifically to TIM-1 which is also the cellular receptor for the hepatitis A virus, and has been implicated in the development of asthma (5 - 7). Among hematopoietic cells, TIM-1 is expressed on activated B and T cells, preferentially in the Th2 subset of CD4⁺ T cells (5, 8). The interaction of TIM-4 with TIM-1 induces costimulatory and hyperproliferative signals in T cells (5).

References

Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454.
Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362.
Shakhov, A.N. et al. (2004) Eur. J. Immunol. 34:494.
Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
Feigelstock, D. et al. (1998) J. Virol. 72:6621.
McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
Khademi, M. et al. (2004) J. Immunol. 172:7169.

Long Name

T Cell Immunoglobulin Mucin-4

Entrez Gene IDs

91937 (Human); 276891 (Mouse); 102140672 (Cynomolgus Monkey)

Alternate Names

FLJ27515; Smuckler; T-cell immunoglobulin and mucin domain containing 4; T-cell immunoglobulin and mucin domain-containing protein 4; T-cell membrane protein 4; TIM4; TIM-4; TIMD4; TIMD-4