Human Tissue alpha-L-Fucosidase/FUCA1 Antibody

Catalog #: AF7039 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7039
AF7039-SP

Detection of Human Tissue alpha ‑L‑Fucosidase/FUCA1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Tissue alpha-L-Fucosidase/FUCA1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Tissue alpha -L-Fucosidase/FUCA1 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Tissue alpha -L-Fucosidase/FUCA1
Gln32-Lys466
Accession # P04066

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-25 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Tissue alpha ‑L‑Fucosidase/FUCA1 by Western Blot. Western blot shows lysates of human thyroid tissue and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Tissue a-L-Fucosidase/FUCA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7039) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Tissue a-L-Fucosidase/FUCA1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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Tissue alpha ‑L‑Fucosidase/FUCA1 in HeLa Human Cell Line. Tissue a-L-Fucosidase/FUCA1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human Tissue a-L-Fucosidase/FUCA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7039) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to lysosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

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Tissue alpha ‑L‑Fucosidase/FUCA1 in Human Liver. Tissue a-L-Fucosidase/FUCA1 was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human Tissue a-L-Fucosidase/FUCA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7039) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lysosomes in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tissue alpha-L-Fucosidase/FUCA1

Fucosylated glycoconjugates play numerous roles in biological processes, such as development, apoptosis and the immune responses, and are involved in the pathology of inflammation, cancer and cystic fibrosis (1, 2, 3, 4). Tissue alpha ‑L‑Fucosidase, also known as FUCA1, is a lysosomal enzyme responsible for hydrolyzing alpha ‑L‑fucose moieties from glycolipids and oligosaccharides (5). The mature enzyme in the liver has a molecular weight of 200 kDa and exists in the native state as a tetramer (6). Defects in FUCA1 are the cause of fucosidosis (7, 8, 9), an autosomal recessive lysosomal storage disease characterized by the accumulation of fucose-containing glycolipids and glycoproteins in various tissues. Serum alpha ‑L‑fucosidase has been identified as a useful marker for oral cancer (10).

References

Hiraishi, K. et al. (1993) Glycobiology 3:381.
Solter, D. and Knowles, B.B. (1978) Proc. Natl. Acad. Sci. USA 75:5565.
Becker, D.J. and Lowe, J.B. (2003) Glycobiology 13:41R.
Liu, T.W. et al. (2009) Proc. Natl. Acad. Sci. USA 106:14581.
Fukushima, H. et al. (1985) Proc. Natl. Acad. Sci. USA 82:1262.
Alhadeff, J.A. et al. (1975) J. Biol. Chem. 250:7106.
O’Brien, J.S. et al. (1987) Enzyme 38:45.
Durand, P. et al. (1969) J. Pediatr. 75:665.
Johnson, K. and Dawson, G. (1985) Biochem. Biophys. Res. Commun. 133:90.
Shah, M. et al. (2008) Cancer 113:336.

Entrez Gene IDs

2517 (Human); 71665 (Mouse); 24375 (Rat)

Alternate Names

Alpha-L-fucosidase 1; Alpha-L-fucosidase I; Alpha-L-fucoside fucohydrolase 1; EC 3.2.1; EC 3.2.1.51; FUCA; FUCA1; fucosidase, alpha-L- 1, tissue; Tissue alphaLFucosidase; Tissue alpha-L-Fucosidase