Human TLR7 PE-conjugated Antibody

Detection of TLR7 in Ramos Human Cell Line by Flow Cytometry. Ramos human Burkitt's lymphoma cell line was stained with Mouse Anti-Human TLR7 PE-conjugated Monoclonal Antibody (Catalog # IC5875P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Detection of Human TLR7 by Flow Cytometry Gating strategy for the detection of TLR-7 and -9 in B cell subsets.PBMC were isolated from whole blood and stained for surface markers before cells were fixed, permeabilised and stained for TLR-7 and -9. FSC and SSC were first used to gate out debris (A) and SSC-A and SSC-H was utilized to eliminate duplicates (B). Further gating was done on CD45 and CD19, to target B cells (C). To separate between the different B cell populations, we gated on CD27 and IgD (D), followed by TLR-7 and -9 expression on these subsets (E). Data from one representative patient is shown. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0120383), licensed under a CC-BY license. Not internally tested by R&D Systems.