Human TMEM59 Antibody

Catalog #: MAB9118 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9118
MAB9118-SP

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Human TMEM59 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TMEM59 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 816004

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E.coli-derived recombinant human TMEM59
Met1-Asn220
Accession # Q9BXS4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

5-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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TMEM59 in HeLa Human Cell Line. TMEM59 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human TMEM59 Monoclonal Antibody (Catalog # MAB9118) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TMEM59

TMEM59 is a 324 aa protein with a 21 amino acid transmembrane domain which localizes to the Golgi compartment where it modulates the O-glycosylation and complex N-glycosylation maturation steps of several proteins including APP, BACE1, SEAP and PRNP. It may retain APP in the Golgi and inhibits amyloid beta generation as well APP cleavage by alpha and beta secretases. TMEM59 also contains a 19 aa peptide that acts as a regulator of autophagy in response to bacterial infection by promoting LC3 activation through interaction with ATG16L1, targeting its own endosomal compartment to lysosomes in response to aggregation during S.areus infection.

References

Ullrich S, et al, J Biol Chem. 2010 Jul 2;285(27):20664
Boada-Romero E, et al, EMBO J. 2013 Feb 20;32(4):566

Long Name

Transmembrane Protein 59

Entrez Gene IDs

9528 (Human); 56374 (Mouse); 100196907 (Rat)

Alternate Names

C1orf8; chromosome 1 open reading frame 8; DCF1; FLJ42977; HSPC001; Liver membrane-bound protein; PRO195; TMEM59; transmembrane protein 59; UNQ169

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

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