Human TMIGD2/CD28H Antibody

Catalog #: MAB83162 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB83162-100
MAB83162-SP

Detection of TMIGD2/CD28H in HEK293 Human Cell Line Transfected with Human TMIGD2/CD28H and eGFP by Flow Cytometry.

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Human TMIGD2/CD28H Antibody Summary

Species Reactivity

Human

Specificity

Detects human TMIGD2/CD28H in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 953743

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell line HEK-293-derived human TMIGD2/CD28H
Met1-Gly150
Accession # Q96BF3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Blockade of Receptor-ligand Interaction

In a functionalflow cytometry test, 50 μg/mL of Mouse Anti-Human TMIGD2/CD28H Antibody(Catalog # MAB83162) will block the binding of 500 ng/mL Recombinant Human B7-H7Fc Chimera (Catalog # 8084-B7)to HEK293 human embryonic kidney cell line transfected with recombinant human TMIGD2/CD28H.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of TMIGD2/CD28H in HEK293 Human Cell Line Transfected with Human TMIGD2/CD28H and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human TMIGD2/CD28H and eGFP was stained with and either (A) Mouse Anti-Human TMIGD2/CD28H Monoclonal Antibody (Catalog # MAB83162) or (B) Mouse IgG_2AIsotype Control (MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). View our protocol for Staining Membrane-associated Proteins.

Blockade of Receptor-ligand Interaction

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B7-H7 binding to TMIGD2/CD28H-transfected HEK293 Human Cell Line is Blocked by Human TMIGD2/CD28H Antibody. In a functional flow cytometry test, Recombinant Human B7-H7 Fc Chimera (8084-B7, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human TMIGD2/CD28H (black dotted line). Binding is completely blocked (orange histogram) by 50 µg/mL of Mouse Anti-Human TMIGD2/CD28H Monoclonal Antibody (Catalog # MAB83162). Mouse IgG_2AIsotype Control (MAB003) at 50 µg/mL was used as a control (blue line). Cells were stained with Mouse Anti-Human IgG₁Fc APC-conjugated Monoclonal Antibody (FAB110A).

Flow Cytometry

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Detection of TMIGD2/CD28H in Human PBMC by Flow Cytometry. Human PBMC were stained with either (A) Mouse Anti-Human TMIGD2/CD28H Monoclonal Antibody (Catalog # MAB83162) or (B) Mouse IgG2A Isotype Control (MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B) and Mouse Anti-Human CD3 PE-conjugated Monoclonal Antibody (FAB100P). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

View Larger

Detection of TMIGD2/CD28H in Human PBMC by Flow Cytometry. Human PBMC were stained with either (A) Mouse Anti-Human TMIGD2/CD28H Monoclonal Antibody (Catalog # MAB83162) or (B) Mouse IgG2A Isotype Control (MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B) and Mouse Anti-Human CD16 PE-conjugated Monoclonal Antibody (FAB2546P). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

View Larger

Detection of TMIGD2/CD28H in Human PBMC by Flow Cytometry. Human PBMC were stained with either (A) Mouse Anti-Human TMIGD2/CD28H Monoclonal Antibody (Catalog # MAB83162) or (B) Mouse IgG2A Isotype Control (MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B) and Mouse Anti-Human CD56 PE-conjugated Monoclonal Antibody (FAB2408P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TMIGD2/CD28H

CD28 homolog (CD28H), also called TMIG2 and IGPR-1, is a 55 kDa glycosylated transmembrane protein that shares approximately 10% amino acid (aa) sequence identity with CD28, CTLA-4, ICOS, and PD-1. CD28H is composed of a single extracellular immunoglobulin variable-like domain (IgV) (aa 23-109), a transmembrane domain (aa 151-171), and a long cytoplasmic domain (aa172-282). CD28H is constitutively expressed on naive T and NK cells. Similar to the interaction of B7 with CD28, the interaction of CD28H with B7-H7 activates the Akt-dependent signaling cascade and promotes the proliferation and activation of newly generated peripheral effector and memory T cells (1, 2). CD28H is additionally expressed in the skin and epithelium lining the lung, airway, mammary gland, and gastrointestinal tract (3). It regulates cellular morphology, focal adhesion contact formation, and cell migration (3, 4). CD28H also participates in angiogenesis in vitro (3). CD28H interacts with multiple cytoskeletal proteins including Actin, Paxillin, SPIN90, CACNB2, and BPAG1. Interactions between the cytoplasmic proline-rich domain of CD28H and SPIN90 modulate the activity of CD28H in both angiogenesis and cell adhesion (3, 5).

References

Zhu, Y. et al. (2013) Nat. Commun. 4:2043.
Parry, R.V. et al. (2003) J.Immunol. 171:166.
Rahimi, N. et al. (2012) Mol. Biol. Cell. 23:1646.
Huang, C. et al. (2003) Nature 424:219.
Kaneko, T. et al. (2008) Front. Biosci. 13:4938.

Long Name

Transmembrane And Immunoglobulin Domain Containing 2

Entrez Gene IDs

126259 (Human)

Alternate Names

CD28H; IGPR1; IGPR-1; MGC23244; TMIGD2; transmembrane and immunoglobulin domain containing 2; transmembrane and immunoglobulin domain-containing protein 2