Human Total EphA1 DuoSet IC ELISA

Human Total EphA1 DuoSet IC ELISA Summary

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Product Features

• Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
• Development protocols are provided to guide further assay optimization
• Assay can be customized to your specific needs
• Available in 2, 5, and 15- (96-well) plate pack sizes
• Economical alternative to Western blot

Kit Content

• Capture Antibody
• Conjugated Detection Antibody
• Calibrated Immunoassay Standard or Control
• Streptavidin-HRP

Other Reagents Required

Product Datasheets

Preparation and Storage

Background: EphA1

Cell migration is influenced by chemodirectants including a large family of receptor tyrosine kinases collectively referred to as Ephs. Ephs are divided into two classes based on their extracellular structure and ligand specificity. Eph receptors that bind preferentially to ephrin-A ligands are referred to as EphA. All Eph receptors contain an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail with one conserved tyrosine residue.