Home / TRA-1-81 / Human TRA-1-81 Antibody

Human TRA-1-81 Antibody

Catalog #: MAB8495
9 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB8495
MAB8495-SP
Detection of TRA-1-81 in BG01V Human Cells by Flow Cytometry.
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Product Details
Citations (9)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TRA-1-81 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TRA-1-81 in flow cytometry.
Source
Monoclonal Mouse IgM Clone # TRA-1-81
Purification
IgM-specific Affinity-purified from hybridoma culture supernatant
Immunogen
Human embryonal carcinoma cell line 2102Ep
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TRA-1-81 antibody in BG01V Human Cells antibody by Flow Cytometry. View Larger

Detection of TRA-1-81 in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Mouse Anti-Human TRA-1-81 Monoclonal Antibody (Catalog # MAB8495, filled histogram) or mouse IgM isotype control antibody (open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0117).

Immunocytochemistry TRA-1-81 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

TRA-1-81 in BG01V Human Embryonic Stem Cells. TRA-1-81 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human TRA-1-81 Monoclonal Antibody (Catalog # MAB8495) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgM Secondary Antibody (red; Catalog # NL019) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRA-1-81

The TRA-1-81 antibody reacts with a high-molecular-mass carbohydrate epitope on the surface of human embryonal carcinoma (EC), embryonic germ (EG), embryonic stem (ES), and induced pluripotent stem (iPS) cells.  TRA-1-81 is an epitope expressed on podocalyxin, also known as Podocalyxin-Like Protein-1 (PCLP1 or PODXL), a type I transmembrane glycoprotein.

Long Name
Tumor-related Antigen-1-81
Entrez Gene IDs
5420 (Human)
Alternate Names
GCTM-2 antigen; GP135; gp200; PC; PCLP; PCLP-1; PDX; Podocalyxin; Podocalyxin-like protein 1; PODXL1; TRA181; TRA-1-81

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Citations for Human TRA-1-81 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Induced pluripotent stem cells from subjects with Lesch-Nyhan disease
    Authors: DJ Sutcliffe, AR Dinasarapu, JE Visser, JD Hoed, F Seifar, P Joshi, I Ceballos-P, T Sardar, EJ Hess, YV Sun, Z Wen, ME Zwick, HA Jinnah
    Scientific Reports, 2021-04-19;11(1):8523.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Generation of three induced pluripotent stem cell lines from postmortem tissue derived following sudden death of a young patient with STXBP1 mutation
    Authors: T Yamamoto, M Otsu, T Okumura, Y Horie, Y Ueno, H Taniguchi, M Ohtaka, M Nakanishi, Y Abe, T Murase, T Umehara, K Ikematsu
    Stem Cell Res, 2019-06-18;39(0):101485.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, IHC
  3. DMSO increases efficiency of genome editing at two non-coding loci
    Authors: G Stratigopo, MC De Rosa, CA LeDuc, RL Leibel, CA Doege
    PLoS ONE, 2018-06-04;13(6):e0198637.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Enhanced efficiency of generating induced pluripotent stem (iPS) cells from human somatic cells by a combination of six transcription factors.
    Authors: Liao J, Wu Z, Wang Y, Cheng L, Cui C, Gao Y, Chen T, Rao L, Chen S, Jia N, Dai H, Xin S, Kang J, Pei G, Xiao L
    Cell Res, 2008-05-01;18(5):600-3.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Induction of pluripotent stem cells from adult human fibroblasts by defined factors.
    Authors: Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S
    Cell, 2007-11-30;131(5):861-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Basic fibroblast growth factor supports undifferentiated human embryonic stem cell growth without conditioned medium.
    Authors: Xu C, Rosler E, Jiang J, Lebkowski J, Gold J, O'Sullivan C, Delavan-Boorsma K, Mok M, Bronstein A, Carpenter M
    Stem Cells, 2005-03-01;23(3):315-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Human feeder layers for human embryonic stem cells.
    Authors: Amit M, Margulets V, Segev H, Shariki K, Laevsky I, Coleman R, Itskovitz-Eldor J
    Biol Reprod, 2003-01-22;68(6):2150-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Surface antigens of human embryonic stem cells: changes upon differentiation in culture.
    Authors: Draper J, Pigott C, Thomson J, Andrews P
    J Anat, 2002-03-01;200(0):249-58.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens.
    Authors: Henderson J, Draper J, Baillie H, Fishel S, Thomson J, Moore H, Andrews P
    Stem Cells, 2002-01-01;20(4):329-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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