Home / TRA-1-81 / Human TRA-1-81 PE-conjugated Antibody

Human TRA-1-81 PE-conjugated Antibody

Catalog #: FAB8495P
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB8495P-025
FAB8495P-100
Detection of TRA-1-81 in BG01V Human Cells by Flow Cytometry.
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Product Details
Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TRA-1-81 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TRA-1-81 in flow cytometry.
Source
Monoclonal Mouse IgM Clone # TRA-1-81
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonal carcinoma cell line 2102Ep
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
BG01V human embryonic stem cells, iPSC cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TRA-1-81 antibody in BG01V Human Cells antibody by Flow Cytometry. View Larger

Detection of TRA-1-81 in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Mouse Anti-Human TRA-1-81 PE-conjugated Monoclonal Antibody (Catalog # FAB8495P, filled histogram) or isotype control antibody (Catalog # IC015P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of TRA-1-81 in iPSC cells by Flow Cytometry. iPSC cells were stained with Mouse Anti-Human TRA-1-81 PE‑conjugated Monoclonal Antibody (Catalog # FAB8495P, filled histogram) or isotype control antibody (Catalog # IC015P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TRA-1-81

The TRA-1-81 antibody reacts with a high-molecular-mass carbohydrate epitope on the surface of human embryonal carcinoma (EC), embryonic germ (EG), embryonic stem (ES), and induced pluripotent stem (iPS) cells.  TRA-1-81 is an epitope expressed on podocalyxin, also known as Podocalyxin-Like Protein-1 (PCLP1 or PODXL), a type I transmembrane glycoprotein.

Long Name
Tumor-related Antigen-1-81
Entrez Gene IDs
5420 (Human)
Alternate Names
GCTM-2 antigen; GP135; gp200; PC; PCLP; PCLP-1; PDX; Podocalyxin; Podocalyxin-like protein 1; PODXL1; TRA181; TRA-1-81

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Citations for Human TRA-1-81 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Establishment of an induced pluripotent stem (iPS) cell line from dermal fibroblasts of an asymptomatic patient with dominant PRPF31 mutation
    Authors: A Terray, V Fort, A Slembrouck, C Nanteau, JA Sahel, S Reichman, I Audo, O Goureau
    Stem Cell Res, 2017-10-07;25(0):26-29.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Generation of an induced pluripotent stem cell (iPSC) line from a patient with autosomal dominant retinitis pigmentosa due to a mutation in the NR2E3 gene
    Authors: A Terray, A Slembrouck, C Nanteau, C Chondroyer, C Zeitz, JA Sahel, I Audo, S Reichman, O Goureau
    Stem Cell Res, 2017-08-05;24(0):1-4.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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