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Human TRACP/PAP/ACP5 Antibody

Catalog #: MAB3948 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3948
MAB3948-SP
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TRACP/PAP/ACP5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TRACP in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 417603
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TRACP/PAP/ACP5
Ala22-Pro320
Accession # P13686
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human TRACP/PAP/ACP5 (Catalog # 3948-AP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRACP/PAP/ACP5

Encoded by the ACP5 gene, Tartrate Resistant Acid Phosphatase (TRACP or TRAP) is also known as Purple Acid Phosphatase (PAP) or Acid Phosphatase 5 (ACP5) (1). The deuced amino acid (aa) sequence of human ACP5 predicts a signal peptide (aa 1-21) and a mature chain (aa 22-325). R&D Systems’ recombinant human ACP5 consists of aa 22-320, without the last 5 residues, RRARP.

ACP5 is expressed at high levels by osteoclasts, macrophages and dendritic cells (2). Two forms, 5a and 5b, circulating in human blood, are derived from different cell types and have different functions. Derived from macrophages and dendritic cells, 5a is a marker of inflammatory conditions. Derived from osteoclasts, 5b is a marker of bone resorption. Compared to 5a, 5b does not contain sialic acid residues, has a higher specific activity and pH optimum, and may be processed into a disulfide-linked dimer (3).

References
  1. Janckila, A.J. and J.M. Halleen (2003) J. Bone Miner. Res. 18:1892.
  2. Halleen, J.M. et al. (2006) Clin. Lab. 52:499.
  3. Janckila, A.J. et al. (2003) J. Bone Miner. Res. 18:1916.
Long Name
Tartrate Resistant Acid Phosphatase 5
Entrez Gene IDs
54 (Human); 11433 (Mouse); 25732 (Rat)
Alternate Names
ACP5; HPAP; TRACP; TRACP5a; TRACP5b; TRACP-5b; TRAP; TrATPase; TTRRAP

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