Human TRAIL R4/TNFRSF10D PerCP-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: TRAILR4/TNFRSF10D

TRAIL-R4, also known as TRAIL-R4 alpha, Decoy Receptor 2 (DcR2) and TNFRSF10D, is a 39-48 kDa member of the TNFR Superfamily of proteins. Mature TRAIL-R4/DcR2 is a type I transmembrane protein 331 amino acids (aa) in length. It contains a 156 aa extracellular domain (ECD) (aa 56-211), and a 154 aa cytoplasmic region that contains a truncated death domain. The presence of this truncation is not believed to allow for a positive signal for the initiation of apoptosis. This has led to its description as a "decoy receptor", suggesting that its function is to simply sequester and decrease the amount of TRAIL available for binding to the two apoptosis-inducing TRAIL receptors, DR4 and DR5. At this time, this view would appear to be unlikely, as it now seems that DcR2 actually complexes with DR5/TRAIL-R2, blocking the activation of Caspase-8, a first step in the initiation of apoptosis. In addition, in a ligand-dependent manner, the presence of DcR2 has also been associated with the increased survival of select leukocytes. Notably, an isoform variant for TRAIL-R4 (called TRAIL-R4 beta ) has been reported that shows an absence of aa 87-124, a deletion that would render it incapable of binding to TRAIL. Cells reported to express TRAIL-R4 are varied, and include eosinophils, NKT cells, CD8⁺ (but not CD4⁺) T cells, colonic columnar epithelium, neutrophils, endometrial columnar epithelium, testicular spermatids and testosterone-secreting Leydig cells, and multiple tumor cell types. A rodent counterpart to TRAIL-R4 has not been reported.

Product Datasheets

Related Research Areas

• B Cell Development and Differentiation
• DNA Damage and Repair
• Neutrophils
• Regulation of Apoptosis by TNF Superfamily Members
• TNF Superfamily
• TNF Superfamily Receptors