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Human TRAIL/TNFSF10 Antibody

Catalog #: AF375
15 Citations Datasheet / COA / SDS
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AF375
AF375-SP
Detection of Human TRAIL/TNFSF10 by Western Blot.
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Citations (15)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TRAIL/TNFSF10 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TRAIL/TNFSF10 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse TRAIL is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TRAIL/TNFSF10
Thr95-Gly281
Accession # P50591
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize TRAIL/TNFSF10-induced cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically <140 ng/mL in the presence of 12 ng/mL Recombinant Human TRAIL/TNFSF10, cross-linking Anti-Mouse His Tag Monoclonal Antibody and Actinomycin D.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TRAIL/TNFSF10 antibody by Western Blot. View Larger

Detection of Human TRAIL/TNFSF10 by Western Blot. Western blot shows lysates of 786-O human renal cell adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TRAIL/TNFSF10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF375) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for TRAIL/TNFSF10 at approximately 27 and 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry TRAIL/TNFSF10 antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

TRAIL/TNFSF10 in Human Brain. TRAIL/TNFSF10 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Goat Anti-Human TRAIL/TNFSF10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF375) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry TRAIL/TNFSF10 antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

TRAIL/TNFSF10 in Human Prostate Cancer Tissue. TRAIL/TNFSF10 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human TRAIL/TNFSF10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF375) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Neutralization View Larger

Cytotoxicity Induced by TRAIL/TNFSF10 and Neutralization by Human TRAIL/TNFSF10 Antibody. In the presence of a cross-linking antibody, Mouse Anti-His Tag Monoclonal Antibody (Catalog # MAB050), and the metabolic inhibitor Actinomycin D, Recombinant Human TRAIL/TNFSF10 (Catalog # 375-TL) induces cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Under these conditions, cytotoxicity elicited by Recombinant Human TRAIL/TNFSF10 (12 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TRAIL/TNFSF10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF375). The ND50is typically <140 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRAIL/TNFSF10

TRAIL is a type II transmembrane protein belonging to the TNF superfamily and is now designated TNFSF10. TRAIL is active as a homotrimer and is produced by a variety of cell types in both the membrane bound form and also as a soluble molecule. It binds to any of the four TRAIL receptors as well as to Osteoprotegerin.

Long Name
TNF-related Apoptosis-inducing Ligand
Entrez Gene IDs
8743 (Human); 22035 (Mouse); 246775 (Rat)
Alternate Names
Apo-2 ligand; APO2L; Apo-2Ltumor necrosis factor (ligand) family, member 10; APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta; CD253 antigen; CD253; Protein TRAIL; TL2; TNF-related apoptosis-inducing ligand; TNFSF10; TRAIL; TRAILTNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor ligand superfamily member 10

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Citations for Human TRAIL/TNFSF10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Interferon-? Overexpression in Adipose Tissue-Derived Stem Cells Induces HepG2 and Macrophage Cell Death in Liver Tumor Organoids via Induction of TNF-Related Apoptosis-Inducing Ligand Expression
    Authors: Yoon, Y;Kim, CW;Kim, MY;Baik, SK;Jung, PY;Eom, YW;
    International journal of molecular sciences
    Species: Human
    Sample Types: Organoids
    Applications: Western Blot
  2. Heparan sulfate promotes TRAIL-induced tumor cell apoptosis
    Authors: Luo, Y;Hao, H;Wang, Z;Ong, C;Dutcher, R;Xu, Y;Liu, J;Pedersen, LC;Xu, D;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Death agonist antibody against TRAILR2/DR5/TNFRSF10B enhances birinapant anti-tumor activity in HPV-positive head and neck squamous cell carcinomas
    Authors: Y An, J Jeon, L Sun, A Derakhshan, J Chen, S Carlson, H Cheng, C Silvin, X Yang, C Van Waes, Z Chen
    Scientific Reports, 2021-03-18;11(1):6392.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Gut-licensed IFN&gamma+ NK cells drive LAMP1+TRAIL+ anti-inflammatory astrocytes
    Authors: LM Sanmarco, MA Wheeler, C Gutiérrez-, CM Polonio, M Linnerbaue, FA Pinho-Ribe, Z Li, F Giovannoni, KV Batterman, G Scalisi, SEJ Zandee, ES Heck, M Alsuwailm, DL Rosene, B Becher, IM Chiu, A Prat, FJ Quintana
    Nature, 2021-01-06;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  5. Adipose Tissue-Derived Mesenchymal Stem Cells Suppress Growth of Huh7 Hepatocellular Carcinoma Cells via Interferon (IFN)-beta -Mediated JAK/STAT1 Pathway in vitro
    Authors: Chun Sung Byun, Soonjae Hwang, Sung-Hun Woo, Moon Young Kim, Jin Suk Lee, Jong In Lee et al.
    International Journal of Medical Sciences
  6. Activated NK cells kill hepatic stellate cells via p38/PI3K signaling in a TRAIL-involved degranulation manner
    Authors: T Li, Y Yang, H Song, H Li, A Cui, Y Liu, L Su, IN Crispe, Z Tu
    J. Leukoc. Biol., 2019-02-12;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Hypoxia-inducible factors have distinct and stage-specific roles during reprogramming of human cells to pluripotency.
    Authors: Mathieu J, Zhou W, Xing Y, Sperber H, Ferreccio A, Agoston Z, Kuppusamy K, Moon R, Ruohola-Baker H
    Cell Stem Cell, 2014-03-20;14(5):592-605.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Potential application of temozolomide in mesenchymal stem cell-based TRAIL gene therapy against malignant glioma.
    Authors: Kim, Seong Mu, Woo, Ji Sun, Jeong, Chang Hy, Ryu, Chung He, Jang, Jae-Deog, Jeun, Sin-Soo
    Stem Cells Transl Med, 2014-01-16;3(2):172-82.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Human cytomegalovirus infection elicits new decidual natural killer cell effector functions.
    Authors: Siewiera J, El Costa H, Tabiasco J, Berrebi A, Cartron G, Le Bouteiller P, Jabrane-Ferrat N
    PLoS Pathog, 2013-04-04;9(4):e1003257.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. KiSS1 metastasis suppressor gene product induces suppression of tyrosine kinase receptor signaling to Akt, tumor necrosis factor family ligand expression, and apoptosis.
    Authors: Navenot JM, Fujii N, Peiper SC
    Mol. Pharmacol., 2009-02-06;75(5):1074-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Osteoprotegerin decreases human osteoclast apoptosis by inhibiting the TRAIL pathway.
    Authors: Chamoux E, Houde N, L'Eriger K, Roux S
    J. Cell. Physiol., 2008-08-01;216(2):536-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC, Neutralization
  12. Cloning and apoptosis-inducing activities of canine and feline TRAIL.
    Authors: Rong S, Cai JH, Andrews J
    Mol. Cancer Ther., 2008-07-01;7(7):2181-91.
    Species: Canine, Feline, Human
    Sample Types: Recombinant Protein
    Applications: Affinity Purification, Western Blot
  13. Cytokines induced during chronic hepatitis B virus infection promote a pathway for NK cell-mediated liver damage.
    Authors: Dunn C, Brunetto M, Reynolds G, Christophides T, Kennedy PT, Lampertico P, Das A, Lopes AR, Borrow P, Williams K, Humphreys E, Afford S, Adams DH, Bertoletti A, Maini MK
    J. Exp. Med., 2007-03-12;204(3):667-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. TNF-related apoptosis-inducing ligand mediates human neuronal apoptosis: links to HIV-1-associated dementia.
    Authors: Ryan LA, Peng H, Erichsen DA, Huang Y, Persidsky Y, Zhou Y, Gendelman HE, Zheng J
    J. Neuroimmunol., 2004-03-01;148(1):127-39.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  15. Apoptosis-mediating receptor-ligand systems in human retinal pigment epithelial cells.
    Authors: Hueber A, Aduckathil S, Kociok N, Welsandt G, Dinslage S, Kirchhof B, Esser PJ
    Chin. Med. J., 2002-06-20;240(7):551-6.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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