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Human TR alpha /NR1A1 Antibody

Catalog #: PP-H2804-00
1 Citation Datasheet / COA / SDS
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PP-H2804-00

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Detection of TR alpha /NR1A1 by Western Blot
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Human TR alpha /NR1A1 Antibody Summary

Species Reactivity
Human
Specificity
This antibody specifically recognizes human TR alpha and cross-reacts with mouse and rat TR alpha. The antibody does not cross-react with human TR beta. Not yet tested in other species.
Source
Monoclonal Mouse IgG2A Clone # H2804
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Recombinant human TR alpha/NR1A1
aa 2-51
Formulation
A liquid formulation in physiologic saline with 0.1% NaN3.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
This antibody can be used at 1 μg/mL under reducingconditions and at 3 μg/mL under non-reducing conditions with the appropriatesecondary reagents to detect human TR alpha.
 
Immunohistochemistry
This antibody can be used at 20 - 50 μg/mL withthe appropriate secondary reagents to detect human TR alpha.
 
Direct ELISA
This antibody can be used at 0.1 μg/mL with the appropriatesecondary reagents to detect human TR alpha.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of TR alpha /NR1A1 by Western Blot View Larger

Detection of TR alpha /NR1A1 by Western Blot Progestins enhanced the growth of THRB-silenced EC cells. (A) THRA or THRB silencing efficiency in RL95-2 and KLE cells. (B,C) Protein expression of TR alpha or TR beta silencing in RL95-2 and KLE cells. (D,G) Cell viability after silencing THRA or THRB in RL95-2 and KLE cells. The cells were treated with si-THRA or si-THRB for 48 h and then cultured in fresh media for 24, 48, 72, and 96 h. (E,F) RL95-2 or (H,I) KLE cells were pretreated with si-THRA or si-THRB for 48 h and then treated with 30 μM MPA (E,H) or NOMAc (F,I); meanwhile, 100 nM T3 was added for 48 h to examine cell viability. The cell viability was normalized to the control, which was set at 100%. The results are presented as mean ± SEM of three independent experiments. TR alpha /THRA, thyroid hormone receptor alpha; TR beta /THRB: thyroid hormone receptor beta; si-Ctrl, negative control treated with siRNA solvent; si-THRA, silenced THRA; si-THRB, silenced THRB. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36293372), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of TR alpha /NR1A1 by Western Blot View Larger

Detection of TR alpha /NR1A1 by Western Blot Progestins enhanced the growth of THRB-silenced EC cells. (A) THRA or THRB silencing efficiency in RL95-2 and KLE cells. (B,C) Protein expression of TR alpha or TR beta silencing in RL95-2 and KLE cells. (D,G) Cell viability after silencing THRA or THRB in RL95-2 and KLE cells. The cells were treated with si-THRA or si-THRB for 48 h and then cultured in fresh media for 24, 48, 72, and 96 h. (E,F) RL95-2 or (H,I) KLE cells were pretreated with si-THRA or si-THRB for 48 h and then treated with 30 μM MPA (E,H) or NOMAc (F,I); meanwhile, 100 nM T3 was added for 48 h to examine cell viability. The cell viability was normalized to the control, which was set at 100%. The results are presented as mean ± SEM of three independent experiments. TR alpha /THRA, thyroid hormone receptor alpha; TR beta /THRB: thyroid hormone receptor beta; si-Ctrl, negative control treated with siRNA solvent; si-THRA, silenced THRA; si-THRB, silenced THRB. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36293372), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: TR alpha/NR1A1

Thyroid Hormone Receptor alpha (TR alpha, THRA; NR1A1) is a member of the Nuclear Hormone Receptor superfamily. The ligands for TR alpha are the thyroid hormones, which exist in two forms: T4 and T3. TR alpha plays a critical role in the differentiation, growth, metabolism and physiology of a wide variety of tissues. The major partners of TRs are the Retinoid X Receptors (RXRs), which strongly enhance their ability to bind to specific DNA sequences and contribute to the specificity of TR.

Long Name
Thyroid Hormone Receptor alpha
Entrez Gene IDs
7067 (Human)
Alternate Names
AR7; c-ERBA-1; c-erbA-alpha; EAR7; EAR-7; EAR-7.1/EAR-7.2; ERBA; ERBA1thyroid hormone receptor, alpha (avian erythroblastic leukemia viral (v-erb-a)oncogene homolog); ERBA-related 7; MGC000261; MGC43240; NR1A 1; NR1A1; NR1A1THRA3; Nuclear receptor subfamily 1 group A member 1; THRA; THRA1ERB-T-1; THRA2; thyroid hormone receptor alpha; thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a)oncogene homolog, avian); TR alpha; triiodothyronine receptor; v-ErbA1; V-erbA-related protein 7

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Citation for Human TR alpha /NR1A1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Thyroid Hormone Receptor beta Knockdown Reduces Suppression of Progestins by Activating the mTOR Pathway in Endometrial Cancer Cells
    Authors: B Ren, J Zhou, Y Hu, R Zhong, Q Lv, S Xie, G Li, B Yang, X Chen, Y Zhu
    International Journal of Molecular Sciences, 2022-10-19;23(20):.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot

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