Home / TRANCE/TNFSF11/RANK L / Human TRANCE/TNFSF11/RANK L Antibody

Human TRANCE/TNFSF11/RANK L Antibody

Catalog #: MAB626
12 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB626-SP
MAB626-500
MAB626-100
Osteoclast-Like Cell Formation Induced by TRANCE/TNFSF11/RANK L and Neutralization by Human TRANCE/TNFSF11/RANK L Antibody.
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Citations (12)
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Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TRANCE/TNFSF11/RANK L Antibody Summary

Species Reactivity
Human
Specificity
Detects human TRANCE/TNFSF11/RANK L in direct ELISAs. No cross-reactivity with recombinant mouse TRANCE is observed.
Source
Monoclonal Mouse IgG2B Clone # 70525
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TRANCE/TNFSF11/RANK L
Gly136-Asp317 (Ala194Gly)
Accession # O14788
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below
Neutralization
Measured by its ability to neutralize TRANCE/TNFSF11/RANK L-induced osteoclast-like cell formation in RAW 264.7 mouse macrophages. The Neutralization Dose (ND50) is typically 0.8-2.5 µg/mL in the presence of 30 ng/mL Recombinant Human TRANCE/TNFSF11/RANK L.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Osteoclast-Like Cell Formation Induced by TRANCE/TNFSF11/RANK L and Neutralization by Human TRANCE/TNFSF11/RANK L Antibody. View Larger

Osteoclast-Like Cell Formation Induced by TRANCE/TNFSF11/RANK L and Neutralization by Human TRANCE/TNFSF11/RANK L Antibody. In the presence of Recombinant Mouse M-CSF (20 ng/mL, Catalog # 416-ML), Recombinant Human TRANCE/TNFSF11/RANK L (Catalog # 390-TN) induces osteoclast-like cell formation in RAW 264.7 mouse macrophages in a dose-dependent manner (orange line), as measured by TRAP (tartrate-resistant acid phosphatase) activity in cell lysates. Under these conditions, osteoclast-like cell formation elicited by Recombinant Human TRANCE/ TNFSF11/RANK L (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TRANCE/TNFSF11/RANK L Monoclonal Antibody (Catalog # MAB626). The ND50 is typically 0.8-2.5 µg/mL.

Immunohistochemistry TRANCE/TNFSF11/RANK L antibody in Human Lymph Node by Immunohistochemistry (IHC-P). View Larger

TRANCE/TNFSF11/RANK L in Human Lymph Node. TRANCE/TNFSF11/RANK L was detected in immersion fixed paraffin-embedded sections of human lymph node using 5 µg/mL Mouse Anti-Human TRANCE/TNFSF11/RANK L Monoclonal Antibody (Catalog # MAB626) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRANCE/TNFSF11/RANK L

TNF-related activation-induced cytokine (TRANCE; also RANKL, OPGL, and ODF) is a 35 kDa (predicted) type II transmembrane glycoprotein and member of the TNF cytokine family. Human TRANCE is 317 amino acids (aa) in length and contains a 47 aa cytoplasmic region, a 21 aa transmembrane region, and a 249 extracellular domain (ECD), which contains two potential sites of N-linked glycosylation. Splicing variants produce three isoforms for human TRANCE. Isoform 1 is the standard form. In isoform 2 lacks aa 1-73, and isoform 3 lacks aa 1-47. TRANCE is expressed highest in the peripheral lymph nodes and weaker in the spleen, peripheral blood leukocytes, bone marrow, heart,placenta, skeletal muscle, stomach, and thyroid. TRANCE plays a role in osteoclast differentiation and activation.

Long Name
TNF-related Activation-induced Cytokine
Entrez Gene IDs
8600 (Human); 21943 (Mouse); 117516 (Rat); 102137946 (Cynomolgus Monkey)
Alternate Names
CD254 antigen; CD254; ODF; OPGL; OPGLOPTB2; Osteoclast differentiation factor; Osteoprotegerin ligand; RANK L; RANKL; RANKLreceptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; sOdf; TNF-related activation-induced cytokine; TNFSF11; TRANCE; TRANCEODFhRANKL2; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11

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Citations for Human TRANCE/TNFSF11/RANK L Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Osteoblasts contribute to a protective niche that supports melanoma cell proliferation and survival
    Authors: Ferguson J, Wilcock DJ, McEntegart S et al.
    Pigment Cell Melanoma Res
  2. Sphingosine 1-phosphate (S1P)/S1P receptor 1 signaling regulates receptor activator of NF-kappa B ligand (RANKL) expression in rheumatoid arthritis
    Authors: Harunori Takeshita, Masayasu Kitano, Tsuyoshi Iwasaki, Sachie Kitano, Sachi Tsunemi, Chieri Sato et al.
    Biochemical and Biophysical Research Communications
  3. Expression of receptor activator of NFkB (RANK) drives stemness and resistance to therapy in ER+HER2- breast cancer
    Authors: I Gomes, BP de Almeida, S Dâmaso, A Mansinho, I Correia, S Henriques, R Cruz-Duart, G Vilhais, P Félix, P Alves, P Corredeira, NL Barbosa-Mo, L Costa, S Casimiro
    Oncotarget, 2020-05-12;11(19):1714-1728.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. The Possible Role of Neutrophils in the Induction of Osteoclastogenesis
    Authors: CGJ Moonen, TJ de Vries, P Rijkschroe, PE Poubelle, EA Nicu, BG Loos
    J Immunol Res, 2019-09-15;2019(0):8672604.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. The effect of vascular endothelial growth factor on osteoclastogenesis in rheumatoid arthritis.
    Authors: Kim H, Kim K, Kim B, Cho M, Lee S
    PLoS ONE, 2015-04-20;10(4):e0124909.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: IHC
  6. Proteinase-activated receptor (PAR)-2 activation impacts bone resorptive properties of human osteoarthritic subchondral bone osteoblasts.
    Authors: Amiable N, Tat SK, Lajeunesse D, Duval N, Pelletier JP, Martel-Pelletier J, Boileau C
    Bone, 2009-03-02;44(6):1143-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. alpha1beta1 integrin and interleukin-7 receptor up-regulate the expression of RANKL in human T cells and enhance their osteoclastogenic function.
    Authors: Gendron S, Boisvert M, Chetoui N, Aoudjit F
    Immunology, 2008-05-13;125(0):359.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. A potential role for Dkk-1 in the pathogenesis of osteosarcoma predicts novel diagnostic and treatment strategies.
    Authors: Lee N, Smolarz AJ, Olson S, David O, Reiser J, Kutner R, Daw NC, Prockop DJ, Horwitz EM, Gregory CA
    Br. J. Cancer, 2007-11-06;97(11):1552-9.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-P, Western Blot
  9. Intraarticular corticosteroids decrease synovial RANKL expression in inflammatory arthritis.
    Authors: Makrygiannakis D, af Klint E, Catrina SB, Botusan IR, Klareskog E, Klareskog L, Ulfgren AK, Catrina AI
    Arthritis Rheum., 2006-05-01;54(5):1463-72.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC, Western Blot
  10. Interface tissue fibroblasts from loose total hip replacement prosthesis produce receptor activator of nuclear factor-kappaB ligand, osteoprotegerin, and cathepsin K.
    Authors: Mandelin J, Li TF, Hukkanen M, Liljestrom M, Salo J, Santavirta S, Konttinen YT
    J. Rheumatol., 2005-04-01;32(4):713-20.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  11. Inflammatory cell infiltrate and RANKL/OPG expression in rheumatoid synovium: comparison with other inflammatory arthropathies and correlation with outcome.
    Authors: Fonseca JE, Cortez-Dias N, Francisco A, Sobral M, Canhao H, Resende C, Castelao W, Macieira C, Sequeira G, Saraiva F, da Silva JA, Carmo-Fonseca M, Viana Queiroz M
    Clin. Exp. Rheumatol., 2005-03-01;23(2):185-92.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  12. Spontaneous Osteoclastogenesis, a risk factor for bone metastasis in advanced luminal A-type breast cancer patients
    Authors: Valeria Fernández Vallone, Francisco Raúl Borzone, Leandro Marcelo Martinez, María Belén Giorello, Hosoon Choi, Federico Dimase et al.
    Frontiers in Oncology

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Reviews for Human TRANCE/TNFSF11/RANK L Antibody

Average Rating: 4.5 (Based on 2 Reviews)

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Human TRANCE/TNFSF11/RANK L Antibody
By Anonymous on 10/17/2021
Application: IHC Sample Tested: Skin tissue Species: Human
Immunohistochemistry Human TRANCE/TNFSF11/RANK L Antibody MAB626
Human TRANCE/TNFSF11/RANK L Antibody
By Alex Wu on 04/21/2017
Application: ELISA Sample Tested: EDTA Plasma,Heparin Plasma,Serum Species: Human