Human Trappin-2/Elafin Antibody Summary
Ala23-Gln117
Accession # P19957
Applications
Human Trappin-2/Elafin Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Neutralization of Trappin‑2/ Elafin Activity by Human Trappin‑2/Elafin Antibody. Recombinant Mouse Neutrophil Elastase/ELA2 (1 µg/mL, Catalog # 4517-SE) activity is measured in the presence of Recombinant Human Trappin-2/ Elafin (1 µg/mL, Catalog # 1747-PI) that has been pre-incubated with increasing concentrations of Goat Anti-Human Trappin-2/Elafin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1747). The ND50 is typically 15.6 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Trappin-2/Elafin
Trappin-2 is the human member of the trappin gene family that contains SLPI (1). Trappin-2 consists of an N-terminal transglutaminase substrate domain (residues 23‑60) and a C-terminal four-disulfide core or whey acidic protein (WAP) domain (residues 72‑117). Elafin or ESI (elastase-specific inhibitor) and SKALP (skin-derived anti-leucoproteinase) are alternative names for Trappin-2 and reflect its protease targets. However, elafin and SKALP sometimes correspond only to the processed form that contains the C-terminal WAP domain of the molecule, which can be isolated naturally. The recombinant human Trappin-2 corresponds to the full‑length form (residues 23‑117), which migrates as two protein bands under SDS-PAGE due to an unidentified mechanism. In addition to its ability to inhibit human neutrophil elastase, it can also be used as a substrate for transglutaminases.
Product Datasheets
Citations for Human Trappin-2/Elafin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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The Global Phosphorylation Landscape of SARS-CoV-2 Infection
Authors: Mehdi Bouhaddou, Danish Memon, Bjoern Meyer, Kris M. White, Veronica V. Rezelj, Miguel Correa Marrero et al.
Cell
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Concerted actions by MMPs, ADAMTS and serine proteases during remodeling of the cartilage callus into bone during osseointegration of hip implants
Authors: J Cassuto, A Folestad, J Göthlin, H Malchau, J Kärrholm
Bone Rep, 2020-09-11;13(0):100715.
Species: Human
Sample Types: Plasma
Applications: ELISA Detection -
High circulating elafin levels are associated with Crohn's disease-associated intestinal strictures
Authors: J Wang, C Ortiz, L Fontenot, Y Xie, W Ho, SA Mattai, DQ Shih, HW Koon
PLoS ONE, 2020-04-14;15(4):e0231796.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
A systems biology examination of the human female genital tract shows compartmentalization of immune factor expression.
Authors: Burgener, Adam, Tjernlund, Annelie, Kaldensjo, Tove, Abou, Max, McCorrister, Stuart, Westmacott, Garrett, Mogk, Kenzie, Ambrose, Emma, Broliden, Kristina, Ball, Blake
J Virol, 2013-02-28;87(9):5141-50.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
Anti-HIV-1 activity of elafin is more potent than its precursor's, trappin-2, in genital epithelial cells.
Authors: Drannik AG, Nag K, Yao XD, Henrick BM, Jain S, Ball TB, Plummer FA, Wachihi C, Kimani J, Rosenthal KL
J. Virol., 2012-02-15;86(8):4599-610.
Species: Human
Sample Types: Vaginal Lavage Fluid
Applications: Immunodepletion -
Elafin, an Elastase-specific Inhibitor, Is Cleaved by Its Cognate Enzyme Neutrophil Elastase in Sputum from Individuals with Cystic Fibrosis.
Authors: Guyot N, Butler MW, McNally P, Weldon S, Greene CM, Levine RL, O'Neill SJ, Taggart CC, McElvaney NG
J. Biol. Chem., 2008-09-17;283(47):32377-85.
Species: Human
Sample Types: Sputum
Applications: ELISA Development -
Anti-HIV-1 Activity of Elafin Depends on Its Nuclear Localization and Altered Innate Immune Activation in Female Genital Epithelial Cells
Authors: Anna G. Drannik, Kakon Nag, Xiao-Dan Yao, Bethany M. Henrick, T. Blake Ball, Francis A. Plummer et al.
PLoS ONE
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