Human Trappin‑2/Elafin Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1747
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)

Human Trappin‑2/Elafin Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Trappin-2/Elafin in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) SLPI, rhTGM‑2, and human Neutrophil Elastase is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Trappin-2/Elafin (R&D Systems, Catalog # 1747-PI)
Ala23-Gln117
Accession # P19957
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Trappin‑2/Elafin (Catalog # 1747-PI)

Human Trappin-2/Elafin Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human Trappin‑2/Elafin Antibody (Catalog # AF1747)

Standard: Recombinant Human Trappin-2/Elafin Protein, CF (Catalog # 1747-PI)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Trappin-2/Elafin

Trappin-2 is the human member of the trappin gene family that contains SLPI (1). Trappin-2 consists of an N-terminal transglutaminase substrate domain (residues 23‑60) and a C-terminal four-disulfide core or whey acidic protein (WAP) domain (residues 72-117). Elafin or ESI (elastase-specific inhibitor) and SKALP (skin-derived anti-leucoproteinase) are alternative names for Trappin-2 and reflect its protease targets. However, elafin and SKALP sometimes correspond only to the processed form that contains the C-terminal WAP domain of the molecule, which can be isolated naturally.

References
  1. Schalkwijk, J. et al. (1999) Biochem. J. 340:569.
Long Name
Elastase Inhibitor, Transglutaminase Substrate, Serine Protease
Entrez Gene IDs
5266 (Human)
Alternate Names
cementoin; Elafin; Elastase-specific inhibitor; ESI; ESIWAP four-disulfide core domain protein 14; Peptidase inhibitor 3; peptidase inhibitor 3, skin-derived; PI3; PI-3; pre-elafin; protease inhibitor 3, skin-derived (SKALP); Protease inhibitor WAP3; SKALP; SKALPELAFIN; Skin-derived antileukoproteinase; Trappin2; Trappin-2; WAP four-disulfide core domain 14; WAP3MGC13613; WFDC14elafin

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Citations for Human Trappin‑2/Elafin Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. The Global Phosphorylation Landscape of SARS-CoV-2 Infection
    Authors: Mehdi Bouhaddou, Danish Memon, Bjoern Meyer, Kris M. White, Veronica V. Rezelj, Miguel Correa Marrero et al.
    Cell
  2. Concerted actions by MMPs, ADAMTS and serine proteases during remodeling of the cartilage callus into bone during osseointegration of hip implants
    Authors: J Cassuto, A Folestad, J Göthlin, H Malchau, J Kärrholm
    Bone Rep, 2020-09-11;13(0):100715.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Detection
  3. Elafin, an Elastase-specific Inhibitor, Is Cleaved by Its Cognate Enzyme Neutrophil Elastase in Sputum from Individuals with Cystic Fibrosis.
    Authors: Guyot N, Butler MW, McNally P, Weldon S, Greene CM, Levine RL, O'Neill SJ, Taggart CC, McElvaney NG
    J. Biol. Chem., 2008-09-17;283(47):32377-85.
    Species: Human
    Sample Types: Sputum
    Applications: ELISA Development, Western Blot

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Reviews for Human Trappin‑2/Elafin Biotinylated Antibody

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Human Trappin‑2/Elafin Biotinylated Antibody
By Anonymous on 05/06/2016
Application: Luminex Development Sample Tested: Cell culture supernatant,cervical vaginal lavage Species: Human