Human TREM-1 Antibody

Catalog #: MAB12781 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB12781-100
MAB12781-SP

Detection of TREM-1 in HEK293 Human Cell Line Transfected with Human TREM-1 and eGFP by Flow Cytometry.

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All TREM-1 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TREM-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TREM-1 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 888111

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human TREM-1
Ala21-Asn150
Accession # Q9NP99

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human TREM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1278) or Mouse Anti-Human TREM-1 Monoclonal Antibody (Catalog # MAB12782).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human TREM-1 DuoSet ELISA Kit (Catalog # DY1278B) for convenient development of a sandwich ELISA or the Human TREM-1 Quantikine ELISA Kit (Catalog # DTRM10C) for a complete optimized ELISA.

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of TREM-1 in HEK293 Human Cell Line Transfected with Human TREM-1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human TREM-1 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human TREM-1 Monoclonal Antibody (Catalog # MAB12871) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

ELISA

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Human TREM‑1 ELISA Standard Curve. Recombinant Human TREM-1 protein was serially diluted 2-fold and captured by Mouse Anti-Human TREM-1 Monoclonal Antibody (Catalog # MAB12781) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human TREM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1278) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

ELISA

View Larger

Human TREM‑1 ELISA Standard Curve. Recombinant Human TREM-1 protein was serially diluted 2-fold and captured by Mouse Anti-Human TREM-1 Monoclonal Antibody (Catalog # MAB12781) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human TREM-1 Monoclonal Antibody (Catalog # MAB12782) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TREM-1

TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. The natural ligand for TREM-1 has not been identified. However, engagement of TREM-1 with an agonist monoclonal antibody leads to expression of IL-8, MCP-1, and TNF-alpha, suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with
LPS-induced shock. Blockade of TREM-1 with a TREM-1/Fc fusion protein protected mice against LPS-induced shock. Human and mouse TREM-1 share approximately 42% amino acid sequence homology (1-3).

References

Bouchon, A. (2000) J. Immunol. 164:4991.
Bouchon, A. (2001) Nature 410:1103.
Nathan, C. and A. Ding (2001) Nature Med. 7:530.

Long Name

Triggering Receptor Expressed on Myeloid Cells 1

Entrez Gene IDs

54210 (Human); 58217 (Mouse)

Alternate Names

CD354 antigen; CD354; TREM1; TREM-1; TREM-1Triggering receptor expressed on monocytes 1; triggering receptor expressed on myeloid cells 1; triggering-receptor TREM1