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Human TREM2 Antibody

Catalog #: MAB1828
7 Citations Datasheet / COA / SDS
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MAB1828
MAB1828-SP
Product Details
Citations (7)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TREM2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TREM2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human TREM1, recombinant mouse (rm) TREM2b, or rmTREM3 is observed.
Source
Monoclonal Mouse IgG2B Clone # 263602
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TREM2
His19-Ser174
Accession # Q9NZC2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human TREM2

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TREM2

TREM2 (Triggering Receptor Expressed on Myeloid cells-2) is a 35 kDa molecular weight type I transmembrane member of the TREM family and Ig superfamily. Mature human TREM2 consists of a 156 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane (TM) domain, and a 35 aa cytoplasmic tail. Within the ECD, human TREM2 shares 73% and 74% aa sequence identity with mouse and rat TREM2, respectively. Two closely related transcripts were reported in mouse and designated TREM2a and TREM2b. Soluble forms of the TREM2 ECD are generated by alternative splicing or proteolytic cleavage, and the cytoplasmic domain can be liberated by gamma-Secretase mediated intramembrane cleavage. It is a pattern recognition receptor that binds anionic ligands. A positively charged lysine within the transmembrane segment allows association with the signal adapter protein, DAP12 to deliver an activating signal that plays a role in both innate and adaptive immune responses, including inhibition of macrophage activation. TREM2 is expressed on macrophages, immature myeloid dendritic cells, osteoclasts, microglia, and adipocytes. It promotes the differentiation and function of osteoclasts, the production of inflammatory cytokines by adipocytes, insulin resistance, and the phagocytic clearance of bacteria. In the CNS, TREM2 binds to ApoE, ApoA1, and ApoB and mediates the clearance of apoptotic neurons, amyloid plaques, and cell debris following demyelination. TREM2 also interacts with and modifies signaling through Plexin A1 on dendritic cells and osteoclasts. Mutations in TREM2 or DAP12 are associated with the development of Alzheimer's disease and Nasu-Hakola disease (NHD/PLOSL) which is characterized by presenile dementia and bone cysts. Soluble TREM2 is elevated in cerebrospinal fluid of patients with active multiple sclerosis (MS), and TREM2 blockade exacerbates disease symptoms in the experimental EAE model of MS.

Long Name
Triggering Receptor Expressed on Myeloid Cells 2
Entrez Gene IDs
54209 (Human); 102133279 (Cynomolgus Monkey)
Alternate Names
PLOSL2; TREM2; TREM-2; Trem2a; Trem2b; Trem2c; TREM-2triggering receptor expressed on myeloid cells 2a; Triggering receptor expressed on monocytes 2; triggering receptor expressed on myeloid cells 2

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Citations for Human TREM2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Human microglia-derived proinflammatory cytokines facilitate human retinal ganglion cell development and regeneration
    Authors: Subramani, M;Lambrecht, B;Ahmad, I;
    Stem cell reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  2. Defects in lysosomal function and lipid metabolism in human microglia harboring a TREM2 loss of function mutation
    Authors: Filipello F, You SF, Mirfakhar FS et al.
    Acta Neuropathologica
  3. TREM2 alters the phagocytic, apoptotic and inflammatory response to A beta 42 in HMC3 cells
    Authors: Rumana Akhter, Yvonne Shao, Shane Formica, Maria Khrestian, Lynn M. Bekris
    Molecular Immunology
  4. An Altered Relationship between Soluble TREM2 and Inflammatory Markers in Young Adults with Down Syndrome: A Preliminary Report
    Authors: Grace E. Weber, Katherine A. Koenig, Maria Khrestian, Yvonne Shao, Elizabeth D. Tuason, Marie Gramm et al.
    The Journal of Immunology
  5. A data-driven approach links microglia to pathology and prognosis in amyotrophic lateral sclerosis
    Authors: J Cooper-Kno, C Green, G Altschuler, W Wei, JJ Bury, PR Heath, M Wyles, C Gelsthorpe, JR Highley, A Lorente-Po, T Beck, K Doyle, K Otero, B Traynor, J Kirby, PJ Shaw, W Hide
    Acta Neuropathol Commun, 2017-03-16;5(1):23.
    Species: Human
    Sample Types: CSF
    Applications: ELISA Development (Capture)
  6. TREM-2 binds to lipooligosaccharides of Neisseria gonorrhoeae and is expressed on reproductive tract epithelial cells.
    Authors: Quan DN, Cooper MD, Potter JL, Roberts MH, Cheng H, Jarvis GA
    Mucosal Immunol, 2008-03-05;1(3):229-38.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Pathological role of osteoclast costimulation in arthritis-induced bone loss.
    Authors: Ochi S, Shinohara M, Sato K, Gober HJ, Koga T, Kodama T, Takai T, Miyasaka N, Takayanagi H
    Proc. Natl. Acad. Sci. U.S.A., 2007-06-25;104(27):11394-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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