Human TREM2 Antibody

Catalog #: MAB11618 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB11618-100
MAB11618-SP

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Human TREM2 Antibody Summary

Species Reactivity

Human

Specificity

Detects recombinant human TREM2 protein in Direct ELISA.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2958E

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line, NS0-derived human TREM2
His19-Ser174
Accession # Q9NZC2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

1-10 µg/mL

Immersion fixed paraffi-embedded sections of human lung and brain cortex

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Detection of TREM2 in Human Lung. TREM2 was detected in immersion fixed paraffin-embedded sections of human lung using Rabbit Anti-Human TREM2 Monoclonal Antibody (Catalog # MAB11618) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry

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Detection of TREM2 in Human Brain Cortex. TREM2 was detected in immersion fixed paraffin-embedded sections of human brain cortex using Rabbit Anti-Human TREM2 Monoclonal Antibody (Catalog # MAB11618) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of microglia. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TREM2

TREM2 (Triggering Receptor Expressed on Myeloid cells-2) is a 35 kDa type I transmembrane member of the TREM family and Ig superfamily (1). Mature human TREM2 consists of a 156 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane (TM) domain, and a 35 aa cytoplasmic tail (2). Within the ECD, human TREM2 shares 73% and 74% aa sequence identity with mouse and rat TREM2, respectively. Soluble forms of the TREM2 ECD are generated by alternative splicing or proteolytic cleavage, and the cytoplasmic domain can be liberated by gamma-Secretase mediated intramembrane cleavage (3). A positively charged lysine within the transmembrane segment allows association with the signal adapter protein, DAP12 and inhibition of macrophage activation (4, 5). TREM2 is expressed on macrophages, immature myeloid dendritic cells, osteoclasts, microglia, and adipocytes (5-9). It promotes the differentiation and function of osteoclasts, the production of inflammatory cytokines by adipocytes, insulin resistance, and the phagocytic clearance of bacteria (9-11). In the CNS, TREM2 binds to ApoE, ApoA1, and ApoB and mediates the clearance of apoptotic neurons, amyloid plaques, and cell debris following demyelination (6-8, 12). TREM2 also interacts with and modifies signaling through Plexin A1 on dendritic cells and osteoclasts (13). Mutations in TREM2 or DAP12 are associated with the development of Alzheimer's disease and Nasu-Hakola disease (NHD/PLOSL) which is characterized by presenile dementia and bone cysts (14, 15). Soluble TREM2 is elevated in cerebrospinal fluid of patients with active multiple sclerosis (MS), and TREM2 blockade exacerbates disease symptoms in the experimental EAE model of MS (16, 17).

References

Painter, M.M. et al. (2015) Mol. Neurodegener. 10:43.
Bouchon, A. et al. (2000) J. Immunol. 164:4991.
Wunderlich, P. et al. (2013) J. Biol. Chem. 288:33027.
Hamerman, J.A. et al. (2006) J. Immunol. 177:2051.
Turnbull, I.R. et al. (2006) J. Immunol. 177:3520.
Akahashi, K. et al. (2005) J. Exp. Med. 201:647.
Atagi, Y. et al. (2015) J. Biol. Chem. 290:26043.
Wang, Y. et al. (2016) J. Exp. Med. 213:667.
Cella, M. et al. (2003) J. Exp. Med. 198:645.
Park, M. et al. (2015) Diabetes 64:117.
N'Diaye, E-N. et al. (2009) J. Cell Biol. 184:215.
Poliani, P.L. et al. (2015) J. Clin. Invest. 125:2161.
Akegahara, N. et al. (2006) Nat. Cell Biol. 8:615.
Colonna, M. and Y. Wang (2016) Nat. Rev. Neurosci. 17:201.
Paloneva, J. et al. (2002) Am. J. Hum. Genet. 71:656.
Piccio, L. et al. (2008) Brain 131:3081.
Piccio, L. et al. (2007) Eur. J. Immunol. 37:1290.

Long Name

Triggering Receptor Expressed on Myeloid Cells 2

Entrez Gene IDs

54209 (Human); 102133279 (Cynomolgus Monkey)

Alternate Names

PLOSL2; TREM2; TREM-2; Trem2a; Trem2b; Trem2c; TREM-2triggering receptor expressed on myeloid cells 2a; Triggering receptor expressed on monocytes 2; triggering receptor expressed on myeloid cells 2