Human TRF2 Antibody

Catalog #: MAB5635 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5635
MAB5635-SP

Detection of Human TRF2 by Western Blot.

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All TRF-2 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TRF2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TRF2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human TRF1 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 689524

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human TRF2
Gln78-Thr238 (predicted)
Accession # Q15554

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human TRF2 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TRF2 Monoclonal Antibody (Catalog # MAB5635) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TRF2 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRF-2

TRF2 (telomeric repeat-binding factor 2; also telomeric DNA-binding protein) is a 55-60 kDa, ubiquitously expressed nuclear protein that participates in telomere homeostasis. TRF-2 binds as a dimer to TTAGGG repeats at ends of chromosomes (telomeres), where it blocks inappropriate activation of the ATM/p53 pathway. It also collaborates with TRF1 to promote normal telomere length. Human TRF2 is 500 amino acids (aa) in length. It contains an N-terminal Arg-rich region (aa 13-30), a dimerization domain (aa 46-112), an NLS (aa 329-333), and a DNA binding HTH myb-type domain (aa 442-499). There is one potential alternate start site 42 aa upstream of the standard start site, and one splice form that shows a 13 aa substitution for aa 239-500. Over aa 78-238, human TRF2 shares 96% aa identity with mouse TRF2.

Long Name

Telomeric Repeat Binding Factor 2

Entrez Gene IDs

7014 (Human); 21750 (Mouse); 361403 (Rat)

Alternate Names

Telomeric DNA-binding protein; telomeric repeat binding factor 2; Telomeric repeat binding protein 2; telomeric repeat-binding factor 2; TERF2; TRBF2; TRF2; TRF-2; TTAGGG repeat binding factor 2; TTAGGG repeat-binding factor 2