Human TRIM21 Antibody

Catalog #: MAB62191 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB62191
MAB62191-SP

Detection of Human TRIM21 by Western Blot.

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All TRIM21 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TRIM21 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TRIM21 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) TRIM5 or rhTRIM32 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 672714

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human TRIM21
Arg195-Pro293
Accession # P19474

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human TRIM21 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HEK293T human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TRIM21 Monoclonal Antibody (Catalog # MAB62191) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TRIM21 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRIM21

TRIM21 (Tripartite motif-containing protein 21; also Ro(SS-A), 52 kDa Ro Protein/Ro52, and RING finger protein 81) is a 52-56 kDa member of the RING finger-B-box-coiled-coil family of proteins. It is an E3 ligase that is found in both nucleus and cytoplasm, where it is often associated with microtubules. TRIM21 ubiquitinates select proteins. In B cells, it targets the Fc fragment of misfolded IgG, providing QC on its production. In macrophages, it acts in a nondegradative manner on IRF8, promoting innate immunity. Human TRIM21 is 475 amino acids (aa) in length. It contains one E3 ligase RING finger domain (aa 16-55), a B-Box type zinc-finger region (aa 92-123), a coiled-coil region (aa 128-238) and a C-terminal SPRY/B30.2 Ig binding domain (aa 268-465). TRIM21 is reported to form trimers. Over aa 195‑293, human TRIM21 exhibits 72% aa identity with mouse TRIM21.

Long Name

Tripartite Motif-containing Protein 21

Entrez Gene IDs

6737 (Human); 20821 (Mouse); 308901 (Rat)

Alternate Names

52 kDa ribonucleoprotein autoantigen Ro/SS-A; 52 kDa Ro/SSA; E3 ubiquitin-protein ligase TRIM21; EC 6.3.2.-; rhTRIM21; RNF81; RNF81RING finger protein 81; ro(SS-A); Ro52; RO52SS-A; Sicca syndrome antigen A; Sjoegren syndrome type A antigen; Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro); SSA1; SSA1SSA; TRIM21; tripartite motif containing 21,52 kDa Ro protein; tripartite motif-containing 21; Tripartite motif-containing protein 21