Home / Tripeptidyl-Peptidase I/TPP1 / Human Tripeptidyl-Peptidase I/TPP1 Antibody

Human Tripeptidyl-Peptidase I/TPP1 Antibody

Catalog #: MAB2237
1 Citation 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2237-SP
MAB2237-100
MAB2237-500
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (1)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Tripeptidyl-Peptidase I/TPP1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Tripeptidyl‑Peptidase I/TPP1 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2A Clone # 254008
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Tripeptidyl‑Peptidase I/TPP1
Ser20-Pro563
Accession # O14773
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Tripeptidyl-Peptidase I/CLN2
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Tripeptidyl-Peptidase I/TPP1, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tripeptidyl-Peptidase I/TPP1

Tripeptidyl-Peptidase I (TPP1), also known as tripeptidyl aminopeptidase, is a lysosomal peptidase which can hydrolyze tripeptides from the N-termini of oligopeptides and also possesses weak endopeptidase activity (1‑3). TPP1 is a serine peptidase with a Ser-Glu-Asp catalytic triad, making it a member of the sedolisin family (4). The TPP1 precursor undergoes autoactivation under conditions of acidic pH (4). TPP1 is expressed in many tissues, with elevated expression in tissues associated with peptide hormone production (5). Mutations in TPP1 have been shown to be a cause of classic late‑infantile neuronal ceroid lipofuscinosis (CLN2), a lysosomal storage disease (6).

References
  1. Sleat, D.E. et al. (1997) Science 277:1802.
  2. Ezaki, J. et al. (1999) J. Neurochem. 72:2573.
  3. Tomkinson, B. (1999) Trends Biochem. Sci. 24:355.
  4. Lin, L. et al. (2001) J. Biol. Chem. 276:2249.
  5. Kida, E. et al. (2001) J. Neuropathol. Exp. Neurol. 60:280.
  6. Sleat, D.E. et al. (1999) Am. J. Hum. Genet. 64:1511.
Entrez Gene IDs
1200 (Human)
Alternate Names
Cell growth-inhibiting gene 1 protein; ceroid-lipofuscinosis, neuronal 2, late infantile (Jansky-Bielschowsky disease); CLN2; CLN2EC 3.4.14.9; growth-inhibiting protein 1; LINCL; LPIC; lysosomal pepstatin insensitive protease; Lysosomal pepstatin-insensitive protease; MGC21297; TPP1; TPP-1; TPP-I; Tripeptidyl aminopeptidase; tripeptidyl peptidase I; tripeptidyl-peptidase 1; TripeptidylPeptidase I; Tripeptidyl-Peptidase I

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citation for Human Tripeptidyl-Peptidase I/TPP1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Characterization of AAV-mediated dorsal root ganglionopathy
    Authors: Nicholas Buss, Lisa Lanigan, Jillynne Zeller, Derek Cissell, Monica Metea, Eric Adams et al.
    Molecular Therapy - Methods & Clinical Development

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human Tripeptidyl-Peptidase I/TPP1 Antibody

Average Rating: 4 (Based on 1 Review)

5 Star
0%
4 Star
100%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human Tripeptidyl-Peptidase I/TPP1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human Tripeptidyl‑Peptidase I/TPP1 Antibody
By Anonymous on 06/04/2019
Application: Func Sample Tested: Trigeminal ganglia Species: Mouse