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Human Troponin I/TNNI3 Antibody

Catalog #: MAB8594
1 Citation Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB8594
MAB8594-SP
Detection of Human, Mouse, and Rat Troponin I/TNNI3 by Western Blot.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Troponin I/TNNI3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Troponin I/TNNI3 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1188B
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Peptide 3
Accession # P19429
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
1 µg/mL
See below
Immunocytochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat Troponin I antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat Troponin I/TNNI3 by Western Blot. Western blot shows lysates of human heart tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Troponin I/TNNI3 Monoclonal Antibody (Catalog # MAB8594) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Troponin I/TNNI3 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human Troponin I antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Troponin I/TNNI3 by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue, loaded at 0.1 mg/mL. A specific band was detected for Troponin I/TNNI3 at approximately 25-35 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human Troponin I/TNNI3 Monoclonal Antibody (Catalog # MAB8594). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunocytochemistry Troponin I antibody in Human Cardiomyocytes by Immunocytochemistry (ICC). View Larger

Troponin I/TNNI3 in Human Cardiomyocytes. Troponin I/TNNI3 was detected in immersion fixed human embryonic stem cells, differentiated into cardiomyocytes using the StemXVivo Cardiomyocyte Differentiation Kit (Catalog # SC032), using Rabbit Anti-Human Troponin I/TNNI3 Monoclonal Antibody (Catalog # MAB8594) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Rabbit IgG Secondary Antibody (green; Catalog # NL006). Cardiac Troponin T was also detected in cardiomyocytes using Mouse Anti-Human Cardiac Troponin T Monoclonal Antibody (Catalog # MAB1874). Cells were co-stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining of Troponin I/TNNI3 was localized to cardiac muscle fibrils. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Troponin I/TNNI3

Troponin I, also known as TNI, is a 24 kDa component of a protein complex on striated muscle thin filaments. Troponin I inhibits the calcium-dependent muscle contraction mediated by Troponins C and T. The expression of cardiac Troponin I (TNNI3) is restricted to cardiac muscle, while TNNI1 and TNNI2 (encoded by distinct genes) are expressed in skeletal muscle. Mutations of cardiac Troponin I are associated with heriditary cardiomyopathy. Human cardiac Troponin I shares 93% amino acid sequence identity with mouse and rat cardiac Troponin I.

Long Name
Troponin I3, Cardiac Type
Entrez Gene IDs
7137 (Human)
Alternate Names
Cardiac troponin I; CMD1FF; CMD2A; CMH7MGC116817; CTnI; familial hypertrophic cardiomyopathy 7; RCM1; TNNC1; TNNC1troponin I, cardiac; TNNI3,; troponin I type 3 (cardiac); troponin I, cardiac muscle; Troponin I/TNNI3

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Citation for Human Troponin I/TNNI3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Robotic High-Throughput Biomanufacturing and Functional Differentiation of Human Pluripotent Stem Cells
    Authors: CA Tristan, P Ormanoglu, J Slamecka, C Malley, PH Chu, VM Jovanovic, Y Gedik, C Bonney, E Barnaeva, J Braisted, SK Mallanna, D Dorjsuren, MJ Iannotti, TC Voss, S Michael, A Simeonov, I Singeç
    bioRxiv, 2020-08-03;0(0):.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, WB, Western Blot

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