Human TROY/TNFRSF19 Alexa Fluor® 647-conjugated Antibody

Catalog #: FAB15481R Datasheet / COA / SDS

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FAB15481R-100UG

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Human TROY/TNFRSF19 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human TROY/TNFRSF19 in direct ELISAs. Stains human TROY/TNFRSF19 transfectants but not irrelevant transfectants in flow cytometry.

Source

Monoclonal Mouse IgG1 Clone # 933202

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TROY/TNFRSF19
Glu30-Leu170
Accession # Q9NS68

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

HEK293 human embryonic kidney cell line transfected with human TROY/TNFRSF19

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TROY/TNFRSF19

TROY (also known as TAJ and TNFRSF19) is a 45‑55 kDa member of the TNF receptor superfamily of molecules (1‑4). It is widely expressed in the embryo, but shows limited expression in adult. In particular, embryonic TROY is found in embryonic epithelium, neural tube, and mesenchyme, and developing neurons of the hippocampus and thalamus (5, 6). In the adult, TROY is found in the nervous system associated with dorsal root and retinal ganglia neurons, astrocytes, and microglia, and in hair follicles where it is expressed by dermal papilla matrix cells (4, 7, 8, 9). In human, TROY has also been found on melanoma cells, not normal melanocytes (10). TROY is reportedly a component of the Nogo-A receptor, taking the place of p75 in a membrane-bound LINGO-1:NgR1:TROY complex (6, 8, 9). This complex purportedly mediates axon repulsion initiated by one of five myelin-associated inhibitory factors (OMpg; CD100; Ephrin B3; Nogo-A; MAG) (6, 11). In the hair follicle, TROY binds LTa and acts with Eda to drive secondary hair follicle development (4, 12). Mature human TROY is a 394 amino acid (aa) type I transmembrane protein (1‑3). It possesses a 141 aa extracellular region (aa 30‑170) that contains three 40 aa TNFR cysteine motifs, and an extended 232 aa cytoplasmic domain (aa 192‑423). There is one potential isoform that shows a two aa substitution for aa 416‑423. The extracellular domains of human and mouse TROY share 90% aa identity.

References

Hu, S. et al. (1999) Genomics 62:103.
Eby, M.T. et al. (2000) J. Biol. Chem. 275:15336.
Kojima, T. et al. (2000) J. Biol. Chem. 275:20742.
Hashimoto, T. et al. (2008) Cell Cycle 7:106.
Pispa, J. et al. (2003) Gene Exp. Patterns 3:675.
Mi, S. (2008) Cytokine Growth Factor Rev. 19:245.
Satoh, J. et al. (2007) Neuropathol. Appl. Neurobiol. 33:99.
Shao, Z. et al. (2005) Neuron 45:353.
Park, J.B. et al. (2000) Neuron 45:345.
Spanjaard, R.A. et al. (2007) Int. J. Cancer 120:1304.
Venkatesh, K. et al. (2007) J. Cell Biol. 177:393.
Pispa, J. et al. (2008) Hum. Mol. Genet. 17:3380.

Long Name

Tumor Necrosis Factor Receptor Superfamily, Member 19

Entrez Gene IDs

55504 (Human); 29820 (Mouse)

Alternate Names

TAJ; TAJ-alpha; TAJToxicity and JNK inducer; TNFRSF19; TRADE; TROY; TROYtumor necrosis factor receptor superfamily member 19; tumor necrosis factor receptor superfamily, member 19

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Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.