Human Trypsin 3/PRSS3 Antibody

Catalog #: MAB37101 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB37101
MAB37101-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Trypsin 3/PRSS3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Trypsin 3/PRSS3 in direct ELISAs and Western blots. Detects both pro and mature forms of Trypsin 3 in Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Trypsin 1, 2, recombinant mouse Trypsin 3, or Chymostrypsin is observed.

Source

Monoclonal Rat IgG_2A Clone # 420012

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Trypsin 3/PRSS3
Val16-Ser247
Accession # NP_002762

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Trypsin 3/PRSS3 (Catalog # 3710-SE)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Trypsin 3/PRSS3

Human Trypsin 3, encoded by the PRSS3 gene, is also known as mesotrypsin (1). Constituting less than 10% of the total trypsin content in normal pancreatic juice, it is one of the three trypsin isoforms produced by the pancreas (2). Compared to Trypsin 1 and 2, one intriguing feature of Trypsin 3 is its resistance to polypeptide trypsin inhibitors, such as the Kunitz-type soybean trypsin inhibitor or the Kazal-type pancreatic secretory trypsin inhibitor. As revealed by the crystal structure, this resistance is likely due to the presence of an arginine residue in place of the highly conserved Gly198 (3). Trypsin 3 is synthesized in the pancreas and secreted into the duodenum lumen, where it is activated by enterokinase. One physiologic function of Trypsin 3 has been proposed to be degradation of trypsin inhibitors, which facilitates the digestion of those foods rich in these proteins (4).The amino acid sequence of human Trypsin 3 shares 76%, 74%, 72% and 67% identity with that of rat, mouse, dog and chicken, respectively.

References

Nyaruhucha, C.N.M. et al. (1997) J. Biol. Chem. 272:10573.
Rinderknecht, H. et al. (1984) Gastroenterology 86:681.
Katona, G. et al. (2002) J. Mol. Biol. 315:1209.
Szmola, R. et al. (2003) J. Biol. Chem. 278:48580.

Entrez Gene IDs

5646 (Human); 22073 (Mouse); 362347 (Rat)

Alternate Names

Brain trypsinogen; EC 3.4.21; EC 3.4.21.4; Mesotrypsin; mesotrypsinogen; MTG; pancreatic trypsinogen III; protease, serine, 3 (mesotrypsin); protease, serine, 3; protease, serine, 4 (trypsin 4, brain); PRSS3; PRSS4; Serine protease 3; Serine protease 4; T9; TRY3MTG; TRY4mesotrypsin; Trypsin 3; Trypsin 4; Trypsin III; Trypsin IV; trypsin-3; Trypsinogen 15; trypsinogen 4; trypsinogen 5; trypsinogen IV