Home / Trypsin 3/PRSS3 / Human Trypsin 3/PRSS3 Antibody

Human Trypsin 3/PRSS3 Antibody

Catalog #: MAB3710
1 Citation Datasheet / COA / SDS
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MAB3710
MAB3710-SP
Neutralization of Trypsin 3/ PRSS3 Activity by Human Trypsin 3/PRSS3 Antibody.
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Human Trypsin 3/PRSS3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Trypsin 3/PRSS3 in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Trypsin-1 or rhTrypsin-2 is observed.
Source
Monoclonal Rat IgG2A Clone # 420025
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Trypsin 3/PRSS3
Val16-Ser247
Accession # NP_002762
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Neutralization
Measured by its ability to neutralize Recombinant Human Active Trypsin 3/PRSS3 (0.03 µg/mL, Catalog # 3714-SE) cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (10 µM, Catalog # ES002). The Neutralization Dose (ND50) is typically 0.15 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Neutralization of Trypsin 3/ PRSS3 Activity by Human Trypsin 3/PRSS3 Antibody. View Larger

Neutralization of Trypsin 3/ PRSS3 Activity by Human Trypsin 3/PRSS3 Antibody. The cleavage of Mca-RPKPVE-Nval-WRK(Dnp)-NH2(10 µM, Catalog # ES002) by Recombinant Human Active Trypsin 3/PRSS3 (0.03 µg/mL, Catalog # 3714-SE) is measured after preincubation with increasing concentrations of Rat Anti-Human Trypsin 3/PRSS3 Monoclonal Antibody (Catalog # MAB3710). The ND50 is typically 0.15 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Trypsin 3/PRSS3

Human Trypsin 3, encoded by the PRSS3 gene, is also known as mesotrypsin (1). Constituting less than 10% of the total trypsin content in normal pancreatic juice, it is one of the three trypsin isoforms produced by the pancreas (2). Compared to trypsin 1 and 2, one intriguing feature of Trypsin 3 is its resistance to polypeptide trypsin inhibitors, such as the Kunitz-type soybean trypsin inhibitor or the Kazal-type pancreatic secretory trypsin inhibitor. As revealed by the crystal structure, this resistance is likely due to the presence of an arginine residue in place of the highly conserved Gly198 (3). Trypsin 3 is synthesized in the pancreas and secreted into the duodenum lumen, where it is activated by enterokinase. One proposed physiological function of Trypsin 3 is degradation of trypsin inhibitors, which facilitates the digestion of those foods rich in these proteins (4).

References
  1. Nyaruhucha, C. N. M. et al. (1997) J. Biol. Chem. 272:10573.
  2. Rinderknecht, H. et al. (1984) Gastroenterology 86:681.
  3. Katona, G. et al. (2002) J. Mol. Biol. 315:1209.
  4. Szmola, R. et al. (2003) J. Biol. Chem. 278:48580.
Entrez Gene IDs
5646 (Human); 22073 (Mouse); 362347 (Rat)
Alternate Names
Brain trypsinogen; EC 3.4.21; EC 3.4.21.4; Mesotrypsin; mesotrypsinogen; MTG; pancreatic trypsinogen III; protease, serine, 3 (mesotrypsin); protease, serine, 3; protease, serine, 4 (trypsin 4, brain); PRSS3; PRSS4; Serine protease 3; Serine protease 4; T9; TRY3MTG; TRY4mesotrypsin; Trypsin 3; Trypsin 4; Trypsin III; Trypsin IV; trypsin-3; Trypsinogen 15; trypsinogen 4; trypsinogen 5; trypsinogen IV

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Citation for Human Trypsin 3/PRSS3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Epigenetic silencing of PRSS3 provides growth and metastasis advantage for human hepatocellular carcinoma
    Authors: Bonan Lin, Xiaomeng Zhou, Shuye Lin, Xiaoyue Wang, Meiying Zhang, Baoping Cao et al.
    Journal of Molecular Medicine

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