Human Tryptase epsilon /BSSP-4 Antibody

Catalog #: MAB5047 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5047
MAB5047-SP

Detection of Human Tryptase epsilon /BSSP‑4 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Tryptase epsilon /BSSP-4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Tryptase epsilon /BSSP-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse Tryptase epsilon /BSSP-4 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 725445

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Tryptase epsilon /BSSP-4
Ala33-Ser317
Accession # Q9GZN4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Tryptase epsilon /BSSP‑4 by Western Blot. Western blot shows lysates of human pancreas tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Tryptase e/BSSP-4 Monoclonal Antibody (Catalog # MAB5047) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Tryptase e/BSSP-4 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry

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Tryptase epsilon /BSSP‑4 in Human Pancreas. Tryptase e/BSSP-4 was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human Tryptase e/BSSP-4 Monoclonal Antibody (Catalog # MAB5047) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in exocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tryptase epsilon/BSSP-4

Tryptase epsilon, also known as brain-specific protease-4 (BSSP-4) and prosemin, is expressed in the brain and epithelium-rich tissues such as the lung and eye (1, 2). BSSP-4 is a serine protease related to trypsin, preferentially hydrolyzing substrates after arginine and lysine residues. However, Tryptase epsilon is less susceptible to inhibition by common trypsin inhibitors such as aprotinin, alpha 1-antitrypsin and secretory leukocyte protease inhibitor (3). Tryptase epsilon efficiently converts pro-urokinase-type plasminogen activator to its mature, active form (3).

References

Wong, G.W. et al. (2004) J. Biol. Chem. 279:2438.
Wong, G.W. et al. (2001) J. Biol. Chem. 276:49169.
Yasuda, S. et al. (2005) Blood 105:3893.

Entrez Gene IDs

64063 (Human); 70835 (Mouse)

Alternate Names

BSP-2; BSSP4; BSSP-4; hBSSP-4; MGC9599; prosemin; protease, serine S1 family member 22; protease, serine, 22; PRSS22; serine protease 22; serine protease 26; SP001LA; Tryptase epsilon; UNQ302/PRO343