Human TSG101 Antibody

Catalog #: MAB114381 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB114381-100
MAB114381-SP

Detection of Human TSG101 by Western Blot.

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All TSG101 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TSG101 Antibody Summary

Species Reactivity

Human

Specificity

Detects TSG101 in direct ELISA.

Source

Monoclonal Mouse IgG₁ Clone # 1065916

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

TSG101 containing synthetic peptide
Accession # Q99816

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

A172 human glioblastoma cell line, MDA‑MB‑231 human breast cancer cell line and K562 human chronic myelogenous leukemia cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human TSG101 by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line, MDA‑MB‑231 human breast cancer cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human TSG101 Monoclonal Antibody (Catalog # MAB114381) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TSG101 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSG101

TSG101 is an essential member of the ESCRT-1 complex which regulates the sorting of ubiquitinated proteins to endosomes, facilitating vesicular trafficking and is implicated in normal development. It is also involved in regulating transcription, protein sorting, biogenesis of multi-vesicular bodies, and viral budding. Dysregulation of ESCRT proteins occurs in the development of various human diseases, including many types of cancers and neurodegenerative diseases. TSG101 is an established cancer-associated gene and truncated aberrantly spliced mRNAs have been reported in various types of cancer. TSG101 is also commonly used as a marker protein for exosomes.

References

Tufan AB, Lazarow K, Kolesnichenko M, Sporbert A, von Kries JP, Scheidereit C. TSG101 associates with PARP1 and is essential for PARylation and DNA damage-induced NF-kappa B activation. EMBO J. 2022 Nov 2;41(21):e110372.
doi: 10.15252/embj.2021110372. Epub 2022 Sep 20. PMID: 36124865; PMCID: PMC9627669.
Chua HH, Kameyama T, Mayeda A, Yeh TH. Cancer-Specifically Re-Spliced TSG101 mRNA Promotes Invasion and Metastasis of Nasopharyngeal Carcinoma. Int J Mol Sci. 2019 Feb 12;20(3):773.
doi: 10.3390/ijms20030773. PMID: 30759747; PMCID: PMC6387056.
Willms E, Johansson HJ, Mäger I, Lee Y, Blomberg KE, Sadik M, Alaarg A, Smith CI, Lehtiö J, El Andaloussi S, Wood MJ, Vader P. Cells release subpopulations of exosomes with distinct molecular and biological properties. Sci Rep. 2016 Mar 2;6:22519.
doi: 10.1038/srep22519. PMID: 26931825; PMCID: PMC4773763.

Long Name

Tumor Susceptibility 101

Entrez Gene IDs

7251 (Human)

Alternate Names

ESCRT-I complex subunit TSG101; TSG10; TSG101; tumor susceptibility gene 10; tumor susceptibility gene 101 protein; tumor susceptibility gene 101; tumor susceptibility protein; VPS23