Human TWEAK/TNFSF12 Antibody Summary
Arg93-His249
Accession # Q4ACW9
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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TWEAK/TNFSF12 Inhibition of Cell Proliferation and Neutralization by Human TWEAK/TNFSF12 Antibody. Recombinant Human TWEAK/TNFSF12 (Catalog # 1090-TW) inhibits proliferation in the the HT-29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line). Activity elicited by Recombinant Human TWEAK/TNFSF12 (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TWEAK/ TNFSF12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1090). The ND50 is typically 2-8 µg/mL.
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TWEAK/TNFSF12 in MDA-MB-231 Human Cell Line. TWEAK/TNFSF12 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using 10 µg/mL Goat Anti-Human TWEAK/TNFSF12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1090) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TWEAK/TNFSF12
TWEAK is a type II transmembrane protein belonging to the TNF superfamily (1). It contains a short cytoplasmic domain (amino acids (aa) 1-18), the transmembrane domain (aa 19-42) and an extracellular domain (aa 43-249). The extracellular domains of human and murine TWEAK share 89% aa sequence identity. A soluble form of TWEAK is generated from the membrane-associated molecules by proteolytic cleavage after Arg 93 suggesting that TWEAK may have long-range effects. TWEAK is expressed widely in many tissues and cells. At least two receptors that bind TWEAK have been identified (2-4). Death Receptor 3 (DR3), also known as TNFRSF12, Apo-3, LARD, WSL-1, or TRAMP, is a TNF receptor superfamily member that is expressed predominantly in tissues with high lymphocyte content (2). It has been suggested that induction of cell death by TWEAK-DR3 interaction involves the activation of NF-kappa B. In cells that lack DR3, alternate pathways of TWEAK-induced cell death mediated by receptors distinct from DR3 have been suggested (5, 6). TWEAK receptor (TWEAKR, alternatively known as FN14), is a novel TNF receptor superfamily member that also binds TWEAK (3, 4). It is a mitogen-inducible gene that is expressed in fibroblasts, hepetocellular carcinomas and endothelial cells. TWEAK-TWEAKR interaction has been shown to play a role in endothelial cell growth and migration. This effect of TWEAK is not mediated by an up-regulation of VEGF (7).
- Chicheportiche, Y. et al. (1997) J. Biol Chem. 272:32401.
- Marsters, S. et al. (1998) Current Biol. 8:525.
- Wiley, S.R. et al. (2001) Immunity, 15:837.
- Feng, S-L.Y. et al. (2000) Am J. Path. 156:1253.
- Nakayama, M. et al. (2002) J. Immunol. 168:734.
- Schneider, P. et al. (1999) Eur. J. Immunol., 29:1785.
- Lynch, C. et al. (1998) J. Biol. Chem. 274:8455.
Product Datasheets
Citations for Human TWEAK/TNFSF12 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Tumor Necrosis Factor‐Like Weak Inducer of Apoptosis or Fn14 Deficiency Reduce Elastase Perfusion‐Induced Aortic Abdominal Aneurysm in Mice
Authors: Carlos Tarín, Valvanera Fernández‐Laso, Cristina Sastre, Julio Madrigal‐Matute, Mónica Gómez, Carlos Zaragoza et al.
Journal of the American Heart Association
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TNF Receptor-Associated Factor 1 is a Major Target of Soluble TWEAK
Authors: José Antonio Carmona Arana, Axel Seher, Manfred Neumann, Isabell Lang, Daniela Siegmund, Harald Wajant
Frontiers in Immunology
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TWEAK/Fn14 pathway modulates properties of a human microvascular endothelial cell model of blood brain barrier.
Authors: Stephan D, Sbai O, Wen J, Couraud P, Putterman C, Khrestchatisky M, Desplat-Jego S
J Neuroinflammation, 2013-01-15;10(0):9.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Potent anti-tumor killing activity of the multifunctional Treg cell line HOZOT against human tumors with diverse origins.
Authors: Inoue T, Tashiro Y, Takeuchi M
Int. J. Oncol., 2011-03-03;38(5):1299-306.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Peripheral artery disease is associated with a high CD163/TWEAK plasma ratio.
Authors: Moreno JA, Dejouvencel T, Labreuche J, Smadja DM, Dussiot M, Martin-Ventura JL, Egido J, Gaussem P, Emmerich J, Michel JB, Blanco-Colio LM, Meilhac O
Arterioscler. Thromb. Vasc. Biol., 2010-03-18;30(6):1253-62.
Species: Human
Sample Types: Cell Culture Supernates, Whole Tissue
Applications: IHC-P, Western Blot
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This antibody was used as the detection in an ELISA with MAB1090 as the capture.
