Human ULBP-2/5/6 APC-conjugated Antibody Summary
Accession # Q9BZM5
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of ULBP-2/5/6 in HT1080 Human Cell Line by Flow Cytometry. HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human ULBP-2/5/6 APC-conjugated Monoclonal Antibody (Catalog # FAB1298A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of Human ULBP-2/5/6 by Flow Cytometry Histograms showing sample flow cytometry profiles for the ligands studied. Bars in each plot indicate the population expressing each aNKR, Gates were set using Unstained, single stained controls, fluorescence minus one, and secondary antibody alone controls. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/1999-4915/9/10/295), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ULBP-2/5/6
ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. The name ULBP derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The genes for ULBPs reside in a cluster of ten related genes, six of which encode potentially functional glycoproteins. ULBP-2 has also been described under the names RaeT1H (retinoic acid early transcript), NKG2DL2, and ALCAN-alpha. ULBP-5 also known as RaeT1G and ULBP-6 also known as RaeT1L. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2‑microglobulin. Some family members, including ULBP-2, are anchored to the membrane via a GPI-linkage, whereas others have transmembrane domains. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. The ULBPs are expressed on some tumor cells and have been implicated in tumor surveillance. Over aa 26-217, ULBP-2 shares 92% and 95% aa sequence identity with the human ULBP-5 and ULBP-6, respectively.
- Cosman, D. et al. (2001) Immunity 14:123.
- Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428.
- Sutherland, C. et al. (2002) J. Immunol. 168:671.
- Steinle, A. et al. (2001) Immunogenetics 53:279.
- Sutherland, C. et al. (2001) Immunol. Rev. 181:185.
- Pende, D. et al. (2002) Cancer Res. 62:6178.
- Radosavljevic, M. et al. (2002) Genomics 79:114.
- NKG2D and its Ligands (2002) www.RnDSystems.com.
Product Datasheets
Citations for Human ULBP-2/5/6 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Fumarate Upregulates Surface Expression of ULBP2/ULBP5 by Scavenging Glutathione Antioxidant Capacity
Authors: RI Høgh, A Droujinine, SH Møller, SD Jepsen, M Mellergaar, L Andresen, S Skov
J. Immunol., 2020-03-06;0(0):.
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NKp30 and NKG2D contribute to natural killer recognition of HIV-infected cells
Authors: Zhao, NQ;Pi, R;Nguyen, DN;Ranganath, T;Seiler, C;Holmes, S;Marson, A;Blish, CA;
bioRxiv : the preprint server for biology
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Tethering of hexokinase 2 to mitochondria promotes resistance of liver cancer cells to natural killer cell cytotoxicity
Authors: Aublin-Gex, A;Jacolin, F;Diaz, O;Jacquemin, C;Marçais, A;Walzer, T;Lotteau, V;Vidalain, PO;Perrin-Cocon, L;
European journal of immunology
Species: Human
Sample Types: Whole Cells, Transduced Whole Cells
Applications: Flow Cytometry -
Development of allogeneic HSC-engineered iNKT cells for off-the-shelf cancer immunotherapy
Authors: Yan-Ruide Li, Yang Zhou, Yu Jeong Kim, Yanni Zhu, Feiyang Ma, Jiaji Yu et al.
Cell Reports Medicine
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Bufalin enhances the killing efficacy of NK cells against hepatocellular carcinoma by inhibiting MICA shedding
Authors: R Fu, F Yu, W Wu, J Liu, J Li, F Guo, L Xu, F Wang, X Cui
International immunopharmacology, 2021-10-19;101(0):108195.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The diterpenoid adenanthin upregulates the expression of natural killer group 2D receptor ligands in hepatocellular carcinoma cells
Authors: X Zhong, H Liao, S Hu, K Luo, H Zhu
Molecular and cellular probes, 2021-07-12;59(0):101759.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
iPSC-Derived Platelets Depleted of HLA Class I Are Inert to Anti-HLA Class I and Natural Killer Cell Immunity
Authors: D Suzuki, C Flahou, N Yoshikawa, I Stirblyte, Y Hayashi, A Sawaguchi, M Akasaka, S Nakamura, N Higashi, H Xu, T Matsumoto, K Fujio, MG Manz, A Hotta, H Takizawa, K Eto, N Sugimoto
Stem Cell Reports, 2019-12-26;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
EGFR-Specific Tyrosine Kinase Inhibitor Modifies NK Cell-Mediated Antitumoral Activity against Ovarian Cancer Cells
Authors: N Mallmann-G, Y Sax, R Kimmig, S Lang, S Brandau
Int J Mol Sci, 2019-09-22;20(19):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The pharmalogical reactivation of p53 function improves breast tumor cell lysis by granzyme B and NK cells through induction of autophagy
Authors: M Chollat-Na, T Ben Safta-, D Haferssas, G Meurice, S Chouaib, J Thiery
Cell Death Dis, 2019-09-20;10(10):695.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Histone Deacetylase Inhibitor Modulates NKG2D Receptor Expression and Memory Phenotype of Human Gamma/Delta T Cells Upon Interaction With Tumor Cells
Authors: Jaydeep Bhat, Samuel Dubin, Alexandra Dananberg, Elgar Susanne Quabius, Juergen Fritsch, C. Marie Dowds et al.
Frontiers in Immunology
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Expression of ligands for activating natural killer cell receptors on cell lines commonly used to assess natural killer cell function
Authors: A Tremblay-M, S Coenraads, Z Kiani, FP Dupuy, NF Bernard
BMC Immunol., 2019-01-29;20(1):8.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Overcoming Target Driven Fratricide for T Cell Therapy
Authors: Eytan Breman, Benjamin Demoulin, Sophie Agaugué, Sebastien Mauën, Alexandre Michaux, Lorraine Springuel et al.
Frontiers in Immunology
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Complete Remission with Reduction of High-risk Clones following Haploidentical NK Cell Therapy against MDS and AML
Authors: AT Björklund, M Carlsten, E Sohlberg, LL Liu, T Clancy, M Karimi, S Cooley, JS Miller, M Klimkowska, M Schaffer, E Watz, KI Wikstrom, P Blomberg, BE Wahlin, M Palma, L Hansson, P Ljungman, E Hellström-, HG Ljunggren, KJ Malmberg
Clin. Cancer Res., 2018-02-14;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Expression levels of UL16 binding protein 1 and natural killer group 2 member D affect overall survival in patients with gastric cancer following gastrectomy
Authors: Ryoji Kamei, Kiyoshi Yoshimura, Shigefumi Yoshino, Moeko Inoue, Tetsuhiko Asao, Masanori Fuse et al.
Oncology Letters
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Expression Profiles of Ligands for Activating Natural Killer Cell Receptors on HIV Infected and Uninfected CD4? T Cells
Authors: A Tremblay-M, J Bruneau, B Lebouché, I Lisovsky, R Song, NF Bernard
Viruses, 2017-10-12;9(10):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
T Cells Infiltrating Diseased Liver Express Ligands for the NKG2D Stress Surveillance System
Authors: Wei-Chen Huang
J. Immunol, 2016-12-28;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
PRL-3 mediates the protein maturation of ULBP2 by regulating the tyrosine phosphorylation of HSP60.
Authors: Leung W, Vong Q, Lin W, Bouck D, Wendt S, Sullivan E, Li Y, Bari R, Chen T, Leung W
J Immunol, 2015-02-16;194(6):2930-41.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The NKG2D ligand ULBP2 is specifically regulated through an invariant chain-dependent endosomal pathway.
Authors: Uhlenbrock F, Hagemann-Jensen M, Kehlet S, Andresen L, Pastorekova S, Skov S
J Immunol, 2014-07-14;193(4):1654-65.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Modulation of NKG2D ligand expression and metastasis in tumors by spironolactone via RXRgamma activation.
Authors: Leung W, Vong Q, Lin W, Janke L, Chen T, Leung W
J Exp Med, 2013-11-04;210(12):2675-92.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Sensitizing primary acute lymphoblastic leukemia to natural killer cell recognition by induction of NKG2D ligands
Authors: Laura Jardine, Sophie Hambleton, Venetia Bigley, Sarah Pagan, Xiao-Nong Wang, Matthew Collin
Leukemia & Lymphoma
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Treatment of a Solid Tumor Using Engineered Drug-Resistant Immunocompetent Cells and Cytotoxic Chemotherapy
Authors: Anindya Dasgupta, Jordan E. Shields, H. Trent Spencer
Human Gene Therapy
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Kaposi's sarcoma-associated herpesvirus ORF54/dUTPase downregulates a ligand for the NK activating receptor NKp44.
Authors: Madrid, Alexis S, Ganem, Don
J Virol, 2012-06-06;86(16):8693-704.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Vesicular Stomatitis Virus Infection Promotes Immune Evasion by Preventing NKG2D-Ligand Surface Expression.
Authors: Jensen H, Andresen L, Nielsen J, Christensen JP, Skov S
PLoS ONE, 2011-08-09;6(8):e23023.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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